An efficient method for the long-term and specific expression of exogenous cDNAs in cultured Purkinje neurons

Journal of Neuroscience Methods

Volumn 200, Issue 2, 2011, Pages 95-105

  Wagner, Wolfgang ^a   McCroskery, Seumas ^a,b   Hammer, John A ^a  

^a NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^b ERMA New Zealand   (New Zealand)

Author keywords

Amaxa nucleofection; Cerebellar culture; Dendritic spines; L7 (Pcp2); Live cell microscopy; Purkinje cells; Transfection

Indexed keywords

BETA ACTIN; COMPLEMENTARY DNA; GREEN FLUORESCENT PROTEIN; PLASMID DNA;

ANIMAL CELL; ARTICLE; CELL FUNCTION; CELL STRUCTURE; CEREBELLUM; DENDRITIC SPINE; ELECTROPORATION; GENE; GENE EXPRESSION; GENE TARGETING; GENETIC TRANSFECTION; MOUSE; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NONHUMAN; NUCLEOFECTION; NUCLEOTIDE SEQUENCE; PCP2 GENE; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PURKINJE CELL; VIRAL GENE DELIVERY SYSTEM;

ACTINS; ANIMALS; CELL DIFFERENTIATION; CELLS, CULTURED; CEREBELLUM; DNA, COMPLEMENTARY; EMBRYO, MAMMALIAN; GENE EXPRESSION REGULATION; GENE EXPRESSION REGULATION, VIRAL; GREEN FLUORESCENT PROTEINS; MICE; MICE, INBRED C57BL; MICROSCOPY, CONFOCAL; NERVE TISSUE PROTEINS; PLASMIDS; PROMOTER REGIONS, GENETIC; PURKINJE CELLS; TIME FACTORS; TRANSFECTION;

EID: 80051584095     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2011.06.006     Document Type: Article

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