Application of novel vectors for CFP-tagging of proteins to study microtubule-associated proteins

Gene

Volumn 173, Issue 1, 1996, Pages 107-111

  Ludin, Beat ^a   Doll, Thierry ^a   Meili, Ruedi ^a   Kaech, Stefanie ^a   Matus, Andrew ^a  

^a FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH   (Switzerland)

Author keywords

Expression vector; Green fluorescent protein; MAP2; Microtubules; Tau protein

Indexed keywords

FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; MICROTUBULE ASSOCIATED PROTEIN; TAU PROTEIN;

CONFERENCE PAPER; CONTROLLED STUDY; DNA TRANSFECTION; EXPRESSION VECTOR; FLUORESCENCE; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING;

EID: 0029892922     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(95)00899-3     Document Type: Conference Paper

References (28)

1. Brand, A.: GFP in Drosophila. Trends Genet. 8 (1995) 324-325
2. Butner, K.A. and Kirschner, M.W.: Tau protein binds to microtubules through a flexible array of distributed weak sites. J. Cell Biol. 115 (1991) 717-730
3. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W. and Prasher, D.C.: Green fluorescent protein as a marker for gene expression. Science 263 (1994) 802-805
4. Chapin, S.J. and Bulinski, J.C.: Microtubule stabilization by assembly-promoting microtubule-associated proteins: a repeat performance. Cell Motil. Cytoskeleton 23 (1992) 236-243
5. Chen, C. and Okayama, H.: High efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell Biol. 7 (1987) 2745-2752
6. Cravchik, A. and Matus, A.: A novel strategy for the immunological tagging of cDNA constructs. Gene 137 (1993) 139-143
7. Edson, K., Weisshaar, B. and Matus, A.: Actin depolymerisation induces process formation on MAP2-transfected non-neuronal cells. Development 117 (1993) 689-700
8. Ferralli, J., Doll, T. and Matus, A.: Sequence analysis of MAP2 function in living cells. J. Cell Sci. 107 (1994) 3115-3125
9. Fregien, N. and Davidson, N.: Activating elements in the promoter region of the chicken β-actin gene. Gene 48 (1986) 1-11
10. Heim, R., Cubitt, A.B. and Tsien, R.Y.: Improved green fluorescence (letter). Nature 373 (1995) 663-664
11. Hodgkinson, S.: GFP in Dictyostelium. Trends Genet. 8 (1995) 327-328
12. Kanai, Y. and Hirokawa, N. : Sorting mechanisms of tau and MAP2 in neurons: suppressed axonal transit of MAP2 and locally regulated microtubule binding. Neuron 14 (1995) 421-432
13. Knops, J., Kosik, K.S., Lee, G., Fardee, J.D., Cohen Gould, L. and McConlogue, L.: Overexpression of tau in a nonneuronal cell induces long cellular processes. J. Cell Biol. 114 (1991) 725-733
14. Kunkel, T.A.: Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82 (1985) 488-492
15. Lee, G. and Brandt, R.: Microtubule-bundling studies revisited: is there a role for MAPs? Trends Cell Biol. 2 (1992) 286-289
16. Lewis, S.A., Ivanov, I.E., Lee, G.H. and Cowan, N.J.: Organization of microtubules in dendrites and axons is determined by a short hydrophobic zipper in microtubule-associated proteins MAP2 and tau. Nature 342 (1989) 498-505
17. Marshall, J., Molloy, R., Moss, G.W., Howe. J.R. and Hughes, T.E.: The jellyfish green fluorescent protein: a new tool for studying ion channel expression and function. Neuron 14 (1995) 211-215
18. Mikhailov, A.V. and Gundersen, G.G.: Centripetal transport of microtubules in motile cells. Cell Motil. Cytoskeleton 32 (1995) 173-186
19. Mitchison, T. and Kirschner, M.: Cytoskeletal dynamics and nerve growth. Neuron 1 (1988) 761-772
20. Munro, S. and Pelham, H.R.B.: A C-terminal signal prevents secretion of luminal ER proteins. Cell 48 (1987) 899-907
21. Olson, K.R., McIntosh, J.R. and Olmsted, J.B.: Analysis of MAP 4 function in living cells using green fluorescent protein (GFP) chimeras. J. Cell Biol. 130 (1995) 639-650
22. Pines, J.: GFP in mammalian cells. Trends Genet. 8 (1995) 326-327
23. Sammak, P.J. and Borisy, G.G.: Direct observation of microtubule dynamics in living cells. Nature 332 (1988) 724-726
24. Shelden, E. and Wadsworth, P.: Observation and quantification of individual microtubule behaviour in vivo: microtubule dynamics are cell-type specific. J. Cell Biol. 120 (1993) 935-945
25. Tanaka, E. and Kirschner, M.W.: The role of microtubules in growth cone turning at substrate boundaries. J. Cell Biol. 128 (1995) 127-137
26. Umeyama, T., Okabe, S., Kanai, Y. and Hirokawa, N.: Dynamics of microtubules bundled by microtubule associated protein 2C (MAP2C). J. Cell Biol. 120 (1993) 451-465
27. Vandenbunder, B. and Borisy, G.G.: Decoration of microtubules by fluorescently labeled microtubule-associated protein 2 (MAP2) does not interfere with their spatial organization and progress through mitosis in living fibroblasts. Cell Motil. Cytoskeleton 6 (1986) 570-579
28. Weisshaar, B., Doll, T. and Matus, A.: Reorganisation of the microtubular Cytoskeleton by embryonic microtubule-associated protein 2 (MAP2c). Development 116 (1992) 1151-1161