Properties and regulation of organic cation transport in freshly isolated mouse proximal tubules analyzed with a fluorescence reader-based method

Pflugers Archiv European Journal of Physiology

Volumn 462, Issue 2, 2011, Pages 359-369

  Holle, Svenja K ^a   Ciarimboli, Giuliano ^a   Edemir, Bayram ^a   Neugebauer, Ute ^a   Pavenstädt, Hermann ^a   Schlatter, Eberhard ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Angiotensin II; Calmodulin; Cation transport; Epithelial transport; Insulin; Microdissection; Microfluorimetry; mOCT1 2 knockout; Mouse; Protein kinase A; Protein kinase C; Proximal tubule; Regulation

Indexed keywords

4 [4 (DIMETHYLAMINO)STYRYL] N METHYLPYRIDINIUM; ANGIOTENSIN II; CALCIUM CALMODULIN DEPENDENT PROTEIN KINASE; CATION; CIMETIDINE; CYCLIC AMP DEPENDENT PROTEIN KINASE; FLUORESCENT DYE; MESSENGER RNA; ORGANIC CATION TRANSPORTER 1; ORGANIC CATION TRANSPORTER 2; ORGANIC CATION TRANSPORTER 3; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE C; PROTEIN KINASE LCK; TETRAPENTYLAMMONIUM; TETRYLAMMONIUM; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BASOLATERAL MEMBRANE; CATION TRANSPORT; CONTROLLED STUDY; FLUORESCENCE READER BASED METHOD; GENE EXPRESSION; HUMAN; HUMAN CELL; IC 50; KIDNEY PROXIMAL TUBULE; MALE; MICROTITER PLATE ASSAY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; REGULATORY MECHANISM; TRANSGENIC MOUSE;

ANIMALS; CATIONS; CELLS, CULTURED; CYCLIC AMP-DEPENDENT PROTEIN KINASES; FLUORESCENT DYES; HUMANS; ION TRANSPORT; KIDNEY TUBULES, PROXIMAL; MALE; MICE; MICE, INBRED C57BL; ORGANIC CATION TRANSPORTER 1; PROTEIN ISOFORMS; PROTEIN KINASE C; RATS; SUBSTRATE SPECIFICITY;

EID: 80051549217     PISSN: 00316768     EISSN: 14322013     Source Type: Journal    
DOI: 10.1007/s00424-011-0969-7     Document Type: Article

References (43)

1. Y Alnouti JS Petrick CD Klaassen 2006 Tissue distribution and ontogeny of organic cation transporters in mice Drug Metab Dispos 34 477 482 16381671 1:CAS:528:DC%2BD28Xit1Sgt7k%3D (Pubitemid 43290907)
2. J Biermann D Lang V Gorboulev H Koepsell A Sindic R Schröter A Zvirbliene H Pavenstädt E Schlatter G Ciarimboli 2006 Characterization of regulatory mechanisms and states of human organic cation transporter 2 Am J Physiol Cell Physiol 290 C1521 C1531 16394027 10.1152/ajpcell.00622.2005 1:CAS:528:DC%2BD28XlvVKrurc%3D (Pubitemid 43830792)
3. HM Bowman JB Hook 1972 Sex differences in organic ion transport by rat kidney Proc Soc Exp Biol Med 141 258 262 5082297 1:CAS:528:DyaE38Xlt1Churw%3D
4. AE Busch S Quester JC Ulzheimer S Waldegger V Gorboulev P Arndt F Lang H Koepsell 1996 Electrogenic properties and substrate specificity of the polyspecific rat cation transporter rOCT1 J Biol Chem 271 32599 32604 8955087 10.1074/jbc.271.51.32599 1:CAS:528:DyaK2sXms1Cq (Pubitemid 27008679)
5. I Cetinkaya G Ciarimboli G Yalcinkaya T Mehrens A Velic JR Hirsch V Gorboulev H Koepsell E Schlatter 2003 Regulation of human organic cation transporter hOCT2 by PKA, PI3K, and calmodulin-dependent kinases Am J Physiol Renal Physiol 284 F293 F302 12388397 1:CAS:528:DC%2BD3sXhtlOqtLc%3D
6. G Ciarimboli 2008 Organic cation transporters Xenobiotica 38 936 971 18668435 10.1080/00498250701882482 1:CAS:528:DC%2BD1cXpt1Snur0%3D
7. G Ciarimboli D Deuster A Knief M Sperling M Holtkamp B Edemir H Pavenstädt C Lanvers-Kaminsky A am Zehnhoff-Dinnesen AH Schinkel H Koepsell H Jürgens E Schlatter 2010 Organic cation transporter 2 mediates cisplatin-induced oto- and nephrotoxicity and is a target for protective interventions Am J Pathol 176 1169 1180 20110413 10.2353/ajpath.2010.090610 1:CAS:528:DC%2BC3cXktVOis7s%3D
8. G Ciarimboli H Koepsell M Iordanova V Gorboulev B Durner D Lang B Edemir R Schröter TV Le E Schlatter 2005 Individual PKC-phosphorylation sites in organic cation transporter 1 determine substrate selectivity and transport regulation J Am Soc Nephrol 16 1562 1570 15829703 10.1681/ASN.2004040256 1:CAS:528:DC%2BD2MXls1eiurw%3D (Pubitemid 41716463)
9. G Ciarimboli E Schlatter 2005 Regulation of organic cation transport Pflugers Arch 449 423 441 15688244 10.1007/s00424-004-1355-5 1:CAS:528:DC%2BD2MXptlaqsQ%3D%3D (Pubitemid 40238877)
10. G Ciarimboli K Struwe P Arndt V Gorboulev H Koepsell E Schlatter JR Hirsch 2004 Regulation of the human organic cation transporter hOCT1 J Cell Physiol 201 420 428 15389554 10.1002/jcp.20081 1:CAS:528:DC%2BD2cXpvVCksLY%3D (Pubitemid 39456441)
11. K Cusi K Maezono A Osman M Pendergrass ME Patti T Pratipanawatr RA DeFronzo CR Kahn LJ Mandarino 2000 Insulin resistance differentially affects the PI 3-kinase- and MAP kinase-mediated signaling in human muscle J Clin Invest 105 311 320 10675357 10.1172/JCI7535 1:CAS:528:DC%2BD3cXhtVSltLo%3D (Pubitemid 30094451)
12. RA DeFronzo 2010 Insulin resistance, lipotoxicity, type 2 diabetes and atherosclerosis: the missing links. The Claude Bernard Lecture 2009 Diabetologia 53 1270 1287 20361178 10.1007/s00125-010-1684-1 1:CAS:528:DC%2BC3cXmsFyrs7w%3D
13. Z Du W Ferguson T Wang 2003 Role of PKC and calcium in modulation of effects of angiotensin II on sodium transport in proximal tubule Am J Physiol Renal Physiol 284 F688 F692 12527554 1:CAS:528:DC%2BD3sXjsVWqtbg%3D
14. V Gorboulev JC Ulzheimer A Akhoundova I Ulzheimer-Teuber U Karbach S Quester C Baumann F Lang AE Busch H Koepsell 1997 Cloning and characterization of two human polyspecific organic cation transporters DNA Cell Biol 16 871 881 9260930 10.1089/dna.1997.16.871 1:CAS:528:DyaK2sXltFGlsbk%3D (Pubitemid 27332696)
15. RM Green K Lo C Sterritt DR Beier 1999 Cloning and functional expression of a mouse liver organic cation transporter Hepatology 29 1556 1562 10216142 10.1002/hep.510290530 1:CAS:528:DyaK1MXjt1Wmsrs%3D (Pubitemid 29211060)
16. D Gründemann V Gorboulev S Gambaryan M Veyhl H Koepsell 1994 Drug excretion mediated by a new prototype of polyspecific transporter Nature 372 549 552 7990927 10.1038/372549a0 (Pubitemid 24368536)
17. H Hohage DM Morth IU Querl J Greven 1994 Regulation by protein kinase C of the contraluminal transport system for organic cations in rabbit kidney S2 proximal tubules J Pharmacol Exp Ther 268 897 901 8114003 1:CAS:528: DyaK2cXhvVyhtrc%3D (Pubitemid 24085891)
18. JW Jonker E Wagenaar S van Eijl AH Schinkel 2003 Deficiency in the organic cation transporters 1 and 2 (Oct1/Oct2 [Slc22a1/Slc22a2]) in mice abolishes renal secretion of organic cations Mol Cell Biol 23 7902 7908 14560032 10.1128/MCB.23.21.7902-7908.2003 1:CAS:528:DC%2BD3sXosFOgtLg%3D (Pubitemid 37271485)
19. H Koepsell 1998 Organic cation transporters in intestine, kidney, liver, and brain Annu Rev Physiol 60 243 266 9558463 10.1146/annurev.physiol.60.1.243 1:CAS:528:DyaK1cXitVOhs7c%3D (Pubitemid 28157873)
20. H Koepsell A Busch V Gorboulev P Arndt 1998 Structure and function of renal organic cation transporters News Physiol Sci 13 11 16 11390752 1:CAS:528:DyaK1cXhslWmsL4%3D
21. H Koepsell H Endou 2004 The SLC22 drug transporter family Pflugers Arch 447 666 676 12883891 10.1007/s00424-003-1089-9 1:CAS:528:DC%2BD2cXhtVygtro%3D (Pubitemid 38241452)
22. H Koepsell V Gorboulev P Arndt 1999 Molecular pharmacology of organic cation transporters in kidney J Membr Biol 167 103 117 9916142 10.1007/s002329900475 1:CAS:528:DyaK1MXhtVehtr0%3D (Pubitemid 29061900)
23. H Koepsell BM Schmitt V Gorboulev 2003 Organic cation transporters Rev Physiol Biochem Pharmacol 150 36 90 12827517 10.1007/s10254-003-0017-x 1:CAS:528:DC%2BD2cXjs1Wmsbw%3D
24. KJ Livak TD Schmittgen 2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method Methods 25 402 408 11846609 10.1006/meth.2001.1262 1:CAS:528:DC%2BD38XhtFelt7s%3D (Pubitemid 34164012)
25. J Loffing C Korbmacher 2009 Regulated sodium transport in the renal connecting tubule (CNT) via the epithelial sodium channel (ENaC) Pflugers Arch 458 111 135 19277701 10.1007/s00424-009-0656-0 1:CAS:528:DC%2BD1MXktVGks7c%3D
26. F Martel E Keating C Calhau D Gründemann E Schömig I Azevedo 2001 Regulation of human extraneuronal monoamine transporter (hEMT) expressed in HEK293 cells by intracellular second messenger systems Naunyn-Schmiedebergs Arch Pharmacol 364 487 495 11770002 10.1007/s002100100476 1:CAS:528: DC%2BD3MXosFGns7s%3D (Pubitemid 33145087)
27. P Meetam C Srimaroeng S Soodvilai V Chatsudthipong 2009 Role of estrogen in renal handling of organic cation, tetraethylammonium: in vivo and in vitro studies Biol Pharm Bull 32 1968 1972 19952413 10.1248/bpb.32.1968 1:CAS:528:DC%2BC3cXhsVOqs7s%3D
28. T Mehrens S Lelleck I Cetinkaya M Knollmann H Hohage V Gorboulev P Boknik H Koepsell E Schlatter 2000 The affinity of the organic cation transporter rOCT1 is increased by protein kinase C-dependent phosphorylation J Am Soc Nephrol 11 1216 1224 10864577 1:CAS:528:DC%2BD3cXltFOjtbY%3D (Pubitemid 30416217)
29. KA Mooslehner ND Allen 1999 Cloning of the mouse organic cation transporter 2 gene, Slc22a2, from an enhancer-trap transgene integration locus Mamm Genome 10 218 224 10051314 10.1007/s003359900976 1:CAS:528: DyaK1MXhs1OlsL8%3D (Pubitemid 29390506)
30. H Motohashi Y Sakurai H Saito S Masuda Y Urakami M Goto A Fukatsu O Ogawa K Inui 2002 Gene expression levels and immunolocalization of organic ion transporters in the human kidney J Am Soc Nephrol 13 866 874 11912245 1:CAS:528:DC%2BD38XjtFSnsb8%3D (Pubitemid 34274082)
31. GT Nagami 1995 Effect of luminal angiotensin II on ammonia production and secretion by mouse proximal tubules Am J Physiol 269 F86 F92 7631835 1:CAS:528:DyaK2MXnt1GrsL0%3D
32. M Okuda H Saito Y Urakami M Takano K Inui 1996 cDNA cloning and functional expression of a novel rat kidney organic cation transporter, OCT2 Biochem Biophys Res Commun 224 500 507 8702418 10.1006/bbrc.1996.1056 1:CAS:528:DyaK28Xktl2qurg%3D (Pubitemid 26268216)
33. G Pietig T Mehrens JR Hirsch I Cetinkaya H Piechota E Schlatter 2001 Properties and regulation of organic cation transport in freshly isolated human proximal tubules J Biol Chem 276 33741 33746 11447227 10.1074/jbc.M104617200 1:CAS:528:DC%2BD3MXmslyltr4%3D
34. L Pirola AM Johnston E van Obberghen 2004 Modulation of insulin action Diabetologia 47 170 184 14722654 10.1007/s00125-003-1313-3 1:CAS:528: DC%2BD2cXjtFCktb4%3D (Pubitemid 38315353)
35. TH Sanderson R Kumar AC Murariu-Dobrin AB Page GS Krause JM Sullivan 2009 Insulin activates the PI3K-Akt survival pathway in vulnerable neurons following global brain ischemia Neurol Res 31 947 958 19203442 10.1179/174313209X382449 1:CAS:528:DC%2BC3cXisVCqtg%3D%3D
36. JA Schafer ML Watkins L Li P Herter S Haxelmans E Schlatter 1997 A simplified method for isolation of large numbers of defined nephron segments Am J Physiol 273 F650 F657 9362342 1:STN:280:DyaK1c%2Fis1Kltw%3D%3D
37. E Schlatter V Mönnich I Cetinkaya T Mehrens G Ciarimboli JR Hirsch C Popp H Koepsell 2002 The organic cation transporters rOCT1 and hOCT2 are inhibited by cGMP J Membr Biol 189 237 244 12395288 10.1007/s00232-002-1023-7 1:CAS:528:DC%2BD38XotFyqs7s%3D (Pubitemid 36930160)
38. S Soodvilai A Chatsudthipong V Chatsudthipong 2007 Role of MAPK and PKA in regulation of rbOCT2-mediated renal organic cation transport Am J Physiol Renal Physiol 293 F21 F27 17327500 10.1152/ajprenal.00043.2007 1:CAS:528:DC%2BD2sXovVygsbk%3D (Pubitemid 47344997)
39. A Stachon H Hohage C Feidt E Schlatter 1997 Characterisation of organic cation transport across the apical membrane of proximal tubular cells with the fluorescent dye 4-Di-1-ASP Cell Physiol Biochem 7 264 274 10.1159/000154881 1:CAS:528:DyaK2sXmsl2rt7o%3D (Pubitemid 27430017)
40. 1 cells Cell Physiol Biochem 6 72 81 10.1159/000154796 1:CAS:528:DyaK28XkvF2rsL4%3D (Pubitemid 26241230)
41. Y Urakami N Nakamura K Takahashi M Okuda H Saito Y Hashimoto K Inui 1999 Gender differences in expression of organic cation transporter OCT2 in rat kidney FEBS Lett 461 339 342 10567723 10.1016/S0014-5793(99)01491-X 1:CAS:528:DyaK1MXntlelt78%3D (Pubitemid 29533359)
42. Y Urakami M Okuda H Saito K Inui 2000 Hormonal regulation of organic cation transporter OCT2 expression in rat kidney FEBS Lett 473 173 176 10812069 10.1016/S0014-5793(00)01525-8 1:CAS:528:DC%2BD3cXjt1ejtb8%3D (Pubitemid 30254285)
43. S Wilde E Schlatter H Koepsell B Edemir S Reuter H Pavenstädt U Neugebauer R Schröter S Brast G Ciarimboli 2009 Calmodulin-associated post-translational regulation of rat organic cation transporter 2 in the kidney is gender dependent Cell Mol Life Sci 66 1729 1740 19330287 10.1007/s00018-009- 9145-z 1:CAS:528:DC%2BD1MXosVGqu7w%3D