Progesterone 5β-reductase of Erysimum crepidifolium: CDNA cloning, expression in Escherichia coli, and reduction of enones with the recombinant protein

Phytochemistry

Volumn 72, Issue 14-15, 2011, Pages 1710-1717

  Munkert, Jennifer ^a   Bauer, Peter ^a   Burda, Edyta ^b   Müller Uri, Frieder ^a   Kreis, Wolfgang ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

^b UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Brassicaceae; Cardenolide biosynthesis; Enzyme characterisation; Erysimum; Heterologous gene expression; Progesterone 5 reductase; Steroid metabolism

Indexed keywords

2 CYCLOHEXENONE; 2-CYCLOHEXEN-1-ONE; CARDENOLIDE; COMPLEMENTARY DNA; CYCLOHEXANONE DERIVATIVE; OXIDOREDUCTASE; PLANT RNA; PROGESTERONE; PROGESTERONE 5 BETA REDUCTASE; PROGESTERONE 5 BETA-REDUCTASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CONSENSUS SEQUENCE; ENZYME SPECIFICITY; ENZYMOLOGY; ERYSIMUM; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY; PLANT LEAF; PROTEIN MOTIF; SEQUENCE ALIGNMENT;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; CARDENOLIDES; CLONING, MOLECULAR; CONSENSUS SEQUENCE; CYCLOHEXANONES; DNA, COMPLEMENTARY; ERYSIMUM; ESCHERICHIA COLI; GENE EXPRESSION; KINETICS; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; PHYLOGENY; PLANT LEAVES; PROGESTERONE; RECOMBINANT PROTEINS; RNA, PLANT; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

ARABIDOPSIS; BRASSICACEAE; DIGITALIS (GENUS); DIGITALIS LANATA; ERYSIMUM; ESCHERICHIA COLI;

EID: 80051547940     PISSN: 00319422     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.phytochem.2011.06.007     Document Type: Article

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