Δ5-3β-hydroxysteroid dehydrogenase (3βHSD) from Digitalis lanata. Heterologous expression and characterisation of the recombinant enzyme

Planta Medica

Volumn 73, Issue 7, 2007, Pages 704-710

  Herl, Vanessa ^a   Frankenstein, Jördis ^a   Meitinger, Nadine ^a   Müller Uri, Frieder ^a   Kreis, Wolfgang ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

5 3 hydroxysteroid dehydrogenase; 5 3 ketosteroid isomerase; Cardenolide biosynthesis; Digitalis lanata; Gene expression; Plantaginaceae; Pregnenolone; Progesterone

Indexed keywords

3 BETA HYDROXYSTEROID DEHYDROGENASE; 5 PREGNENE 3,20 DIONE; CARDENOLIDE; CARDIAC GLYCOSIDE; HYBRID PROTEIN; ISOPROGESTERONE; PREGNENOLONE; PROGESTERONE; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; STEROID REDUCTASE; TESTOSTERONE; UNCLASSIFIED DRUG; 3(OR 17)BETA HYDROXYSTEROID DEHYDROGENASE; DELTA(5) 3 BETA HYDROXYSTEROID DEHYDROGENASE; DELTA(5)-3 BETA-HYDROXYSTEROID DEHYDROGENASE; DIGITALIS; PLANT DNA;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; CELL ASSAY; CELL CULTURE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; FATTY ACID OXIDATION; GENE ISOLATION; GENE SEQUENCE; HETEROLOGOUS EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT LEAF; PROTEIN ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STEROID METABOLISM; AMINO ACID SEQUENCE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENE EXPRESSION; HUMAN; MOLECULAR GENETICS; PHYTOTHERAPY; PLANT SEED; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY;

DIGITALIS (GENUS); DIGITALIS LANATA; PLANTAGINACEAE;

3-HYDROXYSTEROID DEHYDROGENASES; AMINO ACID SEQUENCE; DIGITALIS; DNA, PLANT; GENE EXPRESSION; HUMANS; MOLECULAR SEQUENCE DATA; PHYTOTHERAPY; PLANT LEAVES; POLYMERASE CHAIN REACTION; SEEDS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 34447540345     PISSN: 00320943     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2007-981537     Document Type: Article

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