Separation and purification of a keratinase as pesticide against root-knot nematodes

World Journal of Microbiology and Biotechnology

Volumn 27, Issue 9, 2011, Pages 2147-2153

  Yue, Xiao Yu ^a,e   Zhang, Bin ^b,e   Jiang, Dan Dan ^c   Liu, Yan Juan ^d   Niu, Tian Gui ^e  

^a ZHENGZHOU COLLEGE OF ANIMAL HUSBANDRY ENGINEERING   (China)

^b State Key Laboratory of Biological Feed Engineering   (China)

^c Beijing Homeport Food Ingredients Trading Company   (China)

^d Henan Business College   (China)

^e CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Bacillus; Keratinase; Meloidogyne incognita; Pesticide; Purification

Indexed keywords

AMMONIUM SULFATE; AMMONIUM SULFATE PRECIPITATION; BACILLUS SP; HIGH YIELD; KERATINASE; MALDI TOF MS; MELOIDOGYNE INCOGNITA; MORTALITY RATE; PURIFICATION EFFECT; ROOT KNOT NEMATODE; SDS-PAGE; SEPARATION AND PURIFICATION;

AMMONIUM COMPOUNDS; BACILLI; BACTERIOLOGY; COLUMN CHROMATOGRAPHY; ENZYMES; ION EXCHANGE; LIQUID CHROMATOGRAPHY; PESTICIDES;

PURIFICATION;

BACILLUS SP.; MELOIDOGYNE; MELOIDOGYNE INCOGNITA; MELOIDOGYNE JAVANICA;

EID: 80051547557     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-011-0680-z     Document Type: Article

References (34)

1. Angelis MD, Siragusa S, Berloco M, Caputo L, Settanni L, Alfonsi G, Amerio M, Grandi A, Ragni A, Gobbetti M (2006) Selection of potential probiotic lactobacilli from pig feces to be used as additives in pelleted feeding. Res Microbiol 157: 792-801.
2. Bernal C, Cairo J, Coello N (2006) Purification and characterization of a novel exocellular keratinase from Kocuria rosea. Enzyme Microb Technol 38: 49-54.
3. Bevilacqua L, Ovidi M, Mattia ED, Trovatelli LD, Canganella F (2003) Screening of Bifidobacterium strains isolated from human faeces for antagonistic activities against potentially bacterial pathogens. Microbiol Res 158: 179-185.
4. Bockle B, Galunski B, Müller R (1995) Characterization of a keratinolytic serine protease from Streptomyces pactum DSM40530. Appl Environ Microbiol 61: 3705-3710.
5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 278-284.
6. Cherry JR, Fidantsef AL (2003) Directed evolution of industrial enzymes: an update. Curr Opin Biotechnol 14: 438-443.
7. Elbadri GA, Lee DW, Park JC, Yu HB, Choo HY (2008) Evaluation of various plant extracts for their nematicidal efficacies against juveniles of Meloidogyne incognita. J Asia-Pac Entomol 11: 99-102.
8. El-Refai HA, AbdelNaby MA, Gaballa A, El-Araby MH, Abdel Fattah AF (2005) Improvement of the newly isolated Bacillus pumilus FH9 keratinolytic activity. Pro Biochem 40: 2325-2332.
9. Güllçin I, Küfrevioǧlu OI, Oktay M (2005) Purification and characterization of polyphenol oxidase from nettle (Urtica dioica L.) and inhibition effects of some chemicals on the enzyme activity. J Enzyme Inhib Med Chem 20: 297-302.
10. Jung WJ, Jung SJ, An KN, Jin YL, Park RD, Kim FY, Shon BK, Kim TH (2002) Effect of chitinase-producing Paenibacillus illinoisis KJA-424 on egg hatching of root-knot nematode (Meloidogyne incognita). J Microbiol Biotechnol 12: 865-871.
11. Khan A, Williams KL, Helena KM (2004) Effects of Paecilomyces lilacinus protease and chitinase on the eggshell structures and hatching of Meloidogyne javanica juveniles. Biol Control 31: 346-352.
12. Kojima M, Kanai M, Tominaga M et al (2006) Isolation and characterization of a feather-degrading enzyme from Bacillus pseudofirmus FA30-01. Extremophiles 10: 229-235.
13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
14. Li XQ, Tan A, Voegtline M, Bekele S, Chen CS, Aroian RV (2008) Expression of Cry5B protein from Bacillus thuringiensis in plant roots confers resistance to root-knot nematode. Biol Control 47: 97-102.
15. Lin X, Lee CG, Casale ES, Shih CH (1992) Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain. Appl Environ Microbiol 58: 3271-3275.
16. Lin X, Inglis GD, Yanke LJ, Cheng KJ (1999) Selection and characterization of feather-degrading bacteria from canola meal compost. J Ind Microbiol Biotech 23: 149-153.
17. Mavingui P, Heulin T (1994) In vitro chitinase and antifungal activity of a soil, rhizosphere and rhizoplane population of Bacillus polymyxa. Soil Biol Biochem 26: 801-803.
18. Moallaei H, Zaini F, Larcher G, Beucher B, Bouchara JP (2006) Partial purification and characterization of a 37 kDa extracellular proteinase from Trichophyton vanbreuseghemii. Mycopathologia 161: 369-375.
19. Muhlack RA, Waters EJ, Lim A, O'Neill BK, Colby CB (2007) An alternative method for purification of a major thaumatin-like grape protein (VVTL1) responsible for haze formation in white wine. Asia-Pac J Chem Eng 2: 70-74.
20. Nam GW, Lee DW, Lee HS, Lee NJ, Kim BC, Choe EA, Hwang JK, Suhartono MT, Pyun YR (2002) Native-feather degradation by Fervidobacterium islandicum AW-1, a newly isolated keratinase-producing thermophilic anaerobe. Arch Microbiol 178: 538-547.
21. Nguyen KB, Smart GC Jr (1995a) Morphometrics of infective juveniles of Steinernema spp. and Heterorhabditis bacteriophora (Nemata: Rhabditida). J Nematol 27: 206-212.
22. Nguyen KB, Smart GC Jr (1995b) Scanning electron microscope studies od Steinernema glaseri (Nematoda: Steinernematidae). Nematologica 41: 183-190.
23. Nielsen P, Sorensen J (1997) Multi-target and medium-independent fungal antagonism by hydrolytic enzymes in Paenibacillus polymyxa and Bacillus pumilus strains from barley rhizosphere. FEMS Microbiol Ecol 22: 183-192.
24. Onifade AA, Al-Sane NA, Al-Musallam AA, Al-Zarban S (1998) Potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Biores Technol 66: 1-11.
25. Outtrup H, Jorgensen ST (2002) The importance of Bacillus species in the production of industrial enzymes. In: Berkley R (ed) In applications and systems of Bacillus and relatives. Blackwell Science Inc, Malden, pp 206-218.
26. Ray C, Hussey RS (1995) Evidence for proteolytic processing of a cuticle collagen in a plant-parasite nematode. Mole Biochem Parasitol 72: 243-246.
27. Riffel A, Lucas F, Heeb F, Brandelli A (2003) Characterization of a new keratinolytic bacterium that completely degrades native feather keratin. Arch Microbiol 179: 258-265.
28. Riffel A, Brandelli A, Bellato CM et al (2007) Purification and characterization of a keratinolytic metalloprotease from Chryseobacterium sp. Kr6. J Biotechnol 128: 693-703.
29. Shih JCH (1993) Recent development in poultry waste digestion and feather utilization-a review. Poultry Sci 72: 1617-1620.
30. Suntornsuk W, Tongjun J, Onnim P et al (2005) Purification and characterization of keratinase from a thermotolerant feather-degrading bacterium. World J Microbiol Biotechnol 21: 1111-1117.
31. Tatineni R, Doddapaneni KK, Potumarthi RC et al (2008) Purification and characterization of an alkaline keratinase from Streptomyces sp. Biores Technol 99: 1596-1602.
32. Thys RCS, Brandelli A (2006) Purification and properties of a keratinolytic metalloprotease from Microbacterium sp. J Appl Microbiol 101: 1259-1268.
33. Wang HY, Liu DM, Liu Y, Cheng CF, Ma QY, Huang Q, Zhang YZ (2007) Screening and mutagenesis of a novel Bacillus pumilus strain producing alkaline protease for dehairing. Lett Appl Microbiol 44: 1-6.
34. Zhang B, Jiang DD, Zhou WW, Hao HK, Niu TG (2009) Isolation and characterization of a new Bacillus sp. 50-3 with highly alkaline keratinase activity from Calotes versicolor faeces. World J Microbiol Biotechnol 25: 583-590.