Partial purification and characterization of a 37 kDa extracellular proteinase from Trichophyton vanbreuseghemii

Mycopathologia

Volumn 161, Issue 6, 2006, Pages 369-375

  Moallaei, Hossein ^a   Zaini, Farideh ^a   Larcher, Gérald ^b   Beucher, Bertrand ^b   Bouchara, Jean Philippe ^b  

^a TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b ANGERS UNIVERSITY HOSPITAL   (France)

Author keywords

Enzyme purification; Keratinolytic; Serine proteinase; Trichophyton vanbreuseghemii

Indexed keywords

KERATIN; SERINE PROTEINASE;

ARTICLE; CHEMISTRY; ENZYMOLOGY; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; ITALY; METABOLISM; MICROBIOLOGY; MOLECULAR WEIGHT; PH; PRECIPITATION; TRICHOPHYTON;

CHROMATOGRAPHY, ION EXCHANGE; HYDROGEN-ION CONCENTRATION; ITALY; KERATINS; MOLECULAR WEIGHT; PRECIPITATION; SERINE ENDOPEPTIDASES; SOIL MICROBIOLOGY; TRICHOPHYTON;

FUNGI; TRICHOPHYTON; TRICHOPHYTON VANBREUSEGHEMII;

EID: 33745036131     PISSN: 0301486X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11046-006-0019-8     Document Type: Article

References (29)

1. WW Safranek RD Goos 1982 Degradation of wool by saprophytic fungi Can. J. Microbiol. 28 137 140
2. T Matsumoto 1996 Fungal diseases in dermatology CC Kibbler DW Mackenzie FCJ Odds Principle and Practice of Clinical Mycology Wiley and Sons Ltd New York 103 129
3. RKS Kushwaha N Nigam 1996 Keratinase production by some geophilic fungi Proc Indian Natl Sci Acad B Biol Sci 19 107 109
4. S Bahuguna RK Kushwaha 1989 Hair perforation by keratinophilic fungi Mycoses 32 340 343
5. JA Scott WA Untereiner 2004 Determination of keratin degradation by fungi using keratin azure Med. Mycol. 42 239 246
6. INS Dozie CN Okeke NC Unaeze 1994 A thermostable, alkaline-active, keratinolytic proteinase from Chrysosporium keratinophilum World J Microbiol Biotechnol 10 563 567
7. TM Muhsin AH Aubaid AH al-Duboon 1997 Extracellular enzyme activities of dermatophytes and yeast isolates on solid media Mycoses 40 465 469
8. TM Muhsin TH Salih 2001 Exocellular enzyme activity of dermatophytes and other fungi isolated from ruminants in Southern Iraq Mycopathologia 150 49 52
9. EG DelMar C Largman JW Brodrick MC Geokas 1979 A sensitive new substrate for chymotrypsin Anal. Biochem. 99 316 320
10. MM Bradford 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 248 254
11. UK Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680 685
12. JO Lopes A Salebian SH Alves JP Benevenga 1994 First case of human infection by Trichophyton vanbreuseghemii in Brazil Rev Inst Med Trop Sao Paulo 36 379 380
13. G Larcher JP Bouchara V Annaix F Symoens D Chabasse G Tronchin 1992 Purification and characterization of a fibrinogenolytic serine proteinase from Aspergillus fumigatus culture filtrate FEBS Lett. 308 65 69
14. G Larcher B Cimon F Symoens G Tronchin D Chabasse JP Bouchara 1996 A 33 kDa serine proteinase from Scedosporium apiospermum Biochem. J. 315 119 126
15. O Jousson B Léchenne O Bontems B Mignon U Reichard J Barblan M Quadroni M Monod 2004 Secreted subtilisin gene family in Trichophyton rubrum Gene 339 79 88
16. M Asahi R Lindquist K Fukuyama G Apodaca WL Epstein JH [tmp] McKerrow 1985 Purification and characterization of major extracellular proteinases from Trichophyton rubrum Biochem J 232 139 144
17. LM Qin S Dekio J Jidoi 1992 Some biochemical characteristics of a partially purified extracellular keratinase from Trichophyton schoenleinii Zentralbl Bakteriol 277 236 244
18. R Tsuboi IJ Ko K Takamori H Ogawa 1989 Isolation of a keratinolytic proteinase from Trichophyton mentagrophytes with enzymatic activity at acidic pH Infect Immun 57 3479 3483
19. H Gradisar S Kern J Friedrich 2000 Keratinase of Doratomyces microsporus Appl. Microbiol. Biotechnol. 53 196 200
20. B Mignon M Swinnen JP Bouchara M Hofinger A Nikkels G Pierard C Gerday B Losson 1998 Purification and characterization of 31.5 kDa keratinolytic subtilisin-like serine protease from Microsporum canis and evidence of its secretion in naturally infected cats Med. Mycol 36 395 404
21. HK Malviya RC Rajak SK Hasija 1992 Purification and partial characterization of two extracellular keratinases of Scopulariopsis brevicaulis Mycopathologia 119 161 165
22. V Rojanavanich T Yoshiike R Tsuboi K Takamori H Ogawa 1990 Purification and characterization of an extracellular proteinase from Hendersonula toruloidea Infect Immun 58 2856 2861
23. F Brouta F Descamps T Fett B Losson B Mignon 2001 Purification and characterization of a 43.5 kDa keratinolytic metalloprotease from Microsporum canis Med. Mycol 39 269 275
24. O Jousson B Léchenne O Bontems S Capoccia B Mignon J Barblan M Quadroni M Monod 2004 Multiplication of an ancestral gene encoding secreted fungalysin preceded species differentiation in the dermatophytes Trichophyton and Microsporum Microbiology 150 301 310
25. TM Muhsin AH Aubaid 2001 Partial purification and some biochemical characteristics of exocellular keratinase from T. mentagrophytes var. erinacei Mycopathologia 150 121 125
26. M Monod G Togni L Rahalison E Frenk 1991 Isolation and characterisation of an extracellular alkaline protease of Aspergillus fumigatus J. Med. Microbiol. 35 23 28
27. R Gupta QK Beg P Lorenz 2002 Bacterial alkaline proteases: molecular approaches and industrial applications Appl. Microbiol. Biotechnol. 59 15 32
28. MA EI-Naghy MS El-Ktatny EM Fadl-Allah WW Nazeer 1998 Degradation of chicken feathers by Chrysosporium georgiae Mycopathologia 143 77 84
29. CJ Singh 2002 Optimization of an extracellular protease of Chrysosporium keratinophilum and its potential in bioremediation of keratinic wastes Mycopathologia 156 151 156