Plasma rich in growth factors (PRGF-Endoret) stimulates proliferation and migration of primary keratocytes and conjunctival fibroblasts and inhibits and reverts TGF-β1-induced myodifferentiation

Investigative Ophthalmology and Visual Science

Volumn 52, Issue 9, 2011, Pages 6066-6073

  Anitua, Eduardo ^a   Sanchez, Mikel ^b   Merayo Lloves, Jesus ^c   de La Fuente, Maria ^a   Muruzabal, Francisco ^a   Orive, Gorka ^a  

^a Biotechnology Institute BTI   (Spain)

^b Unidad de Cirugía Artroscópica 'Mikel Sánchez'   (Spain)

^c Fundacion de Investigacion Oftalmologica   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR; PLATELET DERIVED GROWTH FACTOR AB; SCATTER FACTOR; SOMATOMEDIN C; THROMBOSPONDIN 1; TRANSFORMING GROWTH FACTOR BETA1; VASCULOTROPIN;

ARTICLE; BLOOD DONOR; CELL ASSAY; CELL DIFFERENTIATION; CELL MIGRATION; CELL PROLIFERATION; CENTRIFUGATION; CONJUNCTIVA FIBROBLAST; CONTROLLED STUDY; CORNEA CELL; FIBROBLAST; HUMAN; HUMAN CELL; IN VITRO STUDY; INHIBITION KINETICS; LEUKOCYTE COUNT; MYOFIBROBLAST; PRIORITY JOURNAL; SCAR FORMATION; THROMBOCYTE COUNT; THROMBOCYTE RICH PLASMA; TISSUE REGENERATION; WOUND HEALING;

BLOOD DONORS; CELL DIFFERENTIATION; CELL MOVEMENT; CELL PROLIFERATION; CELLS, CULTURED; CONJUNCTIVA; CORNEA; ENZYME-LINKED IMMUNOSORBENT ASSAY; FIBROBLASTS; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MALE; MYOFIBROBLASTS; PLATELET-RICH PLASMA; TRANSFORMING GROWTH FACTOR BETA1;

EID: 80051508466     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.11-7302     Document Type: Article

References (43)

1. Fini ME, Stramer BM. How the cornea heals: cornea-specific repair mechanisms affecting surgical outcomes. Cornea. 2005;24:S2-S11.
2. Wilson SE, Netto M, Ambrosio R Jr. Corneal cells: chatty in development, homeostasis, wound healing, and disease. Am J Ophthalmol. 2003;136:530-536.
3. Woost PG, Brightwell J, Eiferman RA, Schultz GS. Effect of growth factors with dexamethasone on healing of rabbit corneal stromal incisions. Exp Eye Res. 1985;40:47-60.
4. Wilson SE, Lloyd AS, He YG. EGF, basic FGF, and FGF beta-1 messenger RNA production by rabbit corneal epithelial cells. Invest Ophthalmol Vis Sci. 1992;33:1987-1995.
5. Jester JV, Barry-Lane PA, Petroll M, Olsen DR, Cavanagh HD. Inhibition of corneal fibrosis by topical application of blocking antibodies to TGFβ in the rabbit. Cornea. 1997;16:177-187.
6. Chang L, Crowston JG, Cordeiro MF, Akbar AN, Khaw PT. The role of the immune system in conjunctival wound healing after glaucoma surgery. Surv Ophthalmol. 2000;45:49-68.
7. Garrana RM, Petroll WM, Chen WT, et al. Radial keratotomy. II. Role of myofibroblast in corneal wound contraction. Invest Ophthalmol Vis Sci. 1992;33:3271-3282.
8. Jester JV, Petroll WM, Feng W, Essepian J, Cavanagh HD. Radial keratotomy, I. The wound healing process and measurement of incisional gape in two animal models using in vivo confocal microscopy. Invest Ophthalmol Vis Sci. 1992;33:3255-3270.
9. Jester JV, Petroll WM, Cavanagh HD. Corneal stromal wound healing in refractive surgery: the role of myofibroblasts. Prog Retin Eye Res. 1999;18:311-356.
10. Saika S. TGFbeta pathobiology in the eye. Lab Invest. 2006;86: 106-115.
11. Maltseva O, Folger P, Zekaria D, Petridou S, Masur SK. Fibroblast growth factor reversal of the corneal myofibroblast phenotype. Invest Ophthalmol Vis Sci. 2001;42:2490-2495.
12. Netto MV, Mohan RR, Sinha S, Sharma A, Dupps W, Wilson SE. Stromal haze, myofibroblasts, and surface irregularity after PRK. Exp Eye Res. 2006;82:788-797.
13. Mohan RR, Stapleton WM, Sinha S, Netto MV, Wilson SE. A novel method for generating corneal haze in anterior stroma of the mouse eye with the excimer laser. Exp Eye Res. 2008;86:235-240.
14. Sakimoto T, Rosenblatt MI, Azar DT. Laser eye surgery for refractive errors. Lancet. 2006;367:1432-1447.
15. Mietz H, Arnold G, Kirchhof B, Diestelhorst M, Krieglstein GK. Histopathology of episcleral fibrosis after trabeculectomy with and without mitomycin C. Graefes Arch Clin Exp Ophthalmol. 1996; 234:364-368.
16. Skuta GL, Parrish RK II. Wound healing in glaucoma filtering surgery. Surv Ophthalmol. 1987;32:149-170.
17. Gordon JF, Johnson P, Musch DC. Topical fibronectin ophthalmic solution in the treatment of persistent defects of the corneal epithelium. Chiron Vision Fibronectin Study Group. Am J Ophthalmol. 1995;119:281-287.
18. Dellaert MM, Casey TA, Wiffen S, et al. Influence of topical human epidermal growth factor on postkeratoplasty re-epithelialisation. Br J Ophthalmol. 1997;81:391-395.
19. Cordeiro MF, Gay JA, Khaw PT. Human anti-transforming growth factor-β2 antibody: a new glaucoma anti-scarring agent. Invest Ophthalmol Vis Sci. 1999;40:2225-2234.
20. Elner VM, Newman-Casey PA, Patil J, et al. Aberrant wound-healing response in mytomicin C-treated leaking blebs. A histopathologic study. Arch Ophthalmol. 2009;127:1036-1042.
21. Bruno C, Fisher G, Moroi S. Is "scarless wound healing" applicable to glaucoma surgery? Expert Rev Ophthalmol. 2007;2:79-90.
22. Anitua E, Sánchez M, Zalduendo MM, et al. Fibroblastic response to treatment with different preparations rich in growth factors. Cell Prolif. 2009;42:162-170.
23. Anitua E, Sánchez M, Orive G, Andia I. Delivering growth factors for therapeutics. Trends Pharmacol Sci. 2008;29:37-41.
24. Torres J, Tamimi F, Martinez PP, et al. Effect of platelet-rich plasma on sinus lifting: a randomized-controlled clinical trial. J Clin Periodontol. 2009;36:677-687.
25. Anitua E. Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants. 1999;14:529-535.
26. Sánchez M, Anitua E, Azofra J, Andía I, Padilla S, Mujika I. Comparison of surgically repaired Achilles tendon tears using plateletrich fibrin matrices. Am J Sports Med. 2007;35:245-251.
27. Anitua E, Aguirre JJ, Algorta J et al. Effectiveness of autologous preparation rich in growth factors for the treatment of chronic cutaneous ulcers. J Biomed Mater Res B Appl Biomater. 2008;84: 415-421.
28. Zieske JD, Guimaraes SR, Hutcheon AE. Kinetics of keratocyte proliferation in response to epithelial debridement. Exp Eye Res. 2001;72:33-39.
29. Mohan RR, Hutcheon AE, Choi R, et al. Apoptosis, necrosis, proliferation, and myofibroblast generation in the stroma following LASIK and PRK. Exp Eye Res. 2003;76:71-87.
30. Klenkler B, Sheardown H. Growth factors in the anterior segment: role in the tissue maintenance, wound healing and ocular pathology. Exp Eye Res. 2004;79:677-688.
31. Nurden AT, Nurden P, Sanchez M, Andia I, Anitua E. Platelets and wound healing. Front Biosci. 2008;13:3532-3548.
32. Anitua E, Sánchez M, Orive G. Potential of endogenous regenerative technology for in situ regenerative medicine. Adv Drug Deliv Rev. 2010;62:741-752.
33. Masur SK, Dewal HS, Dinh TT, Erenburg I, Petridou S. Myofibroblasts differentiate from fibroblasts when plated at low density. Proc Natl Acad Sci U S A. 1996;93:4219-4223.
34. Wilson SE, Chen L, Mohan RR, Liang Q, Liu J. Expression of HGF, KGF, EGF and receptor messenger RNAs following corneal epithelial wounding. Exp Eye Res. 1999;68:377-397.
35. Wilson SE, Mohan RR, Mohan RR, Ambrosio R Jr, Hong J, Lee J. The corneal wound healing response: cytokine-mediated interaction of the epithelium, stroma, and inflammatory cells. Prog Retin Eye Res. 2001;20:625-637.
36. Li DQ, Tseng SC. Differential regulation of keratinocyte growth factor and hepatocyte growth factor/scatter factor by different cytokines in human corneal and limbal fibroblasts. J Cell Physiol. 1997;172:361-372.
37. Jester JV, Huang J, Petroll WM, Cavanagh HD. TGF beta induced myofibroblast differentiation of rabbit keratocytes requires synergistic TGFbeta, PDGF and integrin signaling. Exp Eye Res. 2002; 75:645-657.
38. Song QH, Klepeis VE, Nugent MA, Trinkaus-Randall V. TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing. Mol Pathol. 2002;55:164-176.
39. Wilson SE, Chaurasia SS, Medeiros FW. Apoptosis in the initiation, modulation and termination of the corneal wound healing response. Exp Eye Res. 2007;85:305-311.
40. Alio JL, Pastor S, Ruiz-Colecha J, Rodriguez A, Artola A. Treatment of ocular surface syndrome after LASIK with autologous plateletrich plasma. J Refract Surg. 2007;23:617-619.
41. Alio JL, Colecha JR, Pastor S, Rodriguez A, Artola A. Symptomatic dry eye treatment with autologous platelet-rich plasma. Ophthalmic Res. 2007;39:124-129.
42. Alio JL, Abad M, Artola A, Rodriguez-Prats JL, Pastor S, Ruiz-Colecha J. Use of autologous platelet-rich plasma in the treatment of dormant corneal ulcers. Ophthalmology. 2007;114:1286-1293.
43. López-Plandolit S, Morales MC, Freire V, Etxebarría J, Durán JA. Plasma rich in growth factors as a therapeutic agent for persistent corneal epithelial defects. Cornea. 2010;29:843-848.