Evaluation of okadaic acid-induced genotoxicity in human cells using the micronucleus test and γh2AX analysis

Journal of Toxicology and Environmental Health - Part A: Current Issues

Volumn 74, Issue 15-16, 2011, Pages 980-992

  Valdiglesias, Vanessa ^a   Laffon, Blanca ^a   Pasaro, Eduardo ^a   Mendez, Josefina ^a  

^a UNIVERSITY OF A CORUÑA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ANEUGEN; CLASTOGEN; HISTONE H2AX; OKADAIC ACID;

ARTICLE; CANCER CELL CULTURE; EVALUATION; GAMMAH2AX ANALYSIS; GENOTOXICITY; HEPATOMA CELL; HUMAN; HUMAN CELL; LEUKOCYTE; MICRONUCLEUS TEST; MUTAGENICITY; NEUROBLASTOMA CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS;

CELL LINE; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; HISTONES; HUMANS; LEUKOCYTES, MONONUCLEAR; MICRONUCLEUS TESTS; OKADAIC ACID;

ALGAE;

EID: 79961078752     PISSN: 15287394     EISSN: 10872620     Source Type: Journal    
DOI: 10.1080/15287394.2011.582026     Document Type: Article

References (60)

1. Andrievski, A., and Wilkins, R. C. 2009. The response of gamma-H2AX in human lym-phocytes and lymphocytes subsets measured in whole blood cultures. Int. J. Radiat. Biol. 85: 369-76.
2. Antonelli, F., Belli, M., Cuttone, G., Dini, V., Esposito, G., Simone, G., Sorrentino, E., Tabocchini, and M. A. 2005. Induction and repair of DNA double-strand breaks in human cells: dephosphorylation of his-tone H2AX and its inhibition by calyculin A. Radiat Res. 164: 514-17. (Pubitemid 41368928)
3. Ao, L., Liu, J. Y., Gao, L. H., Liu, S. X., Yang, M. S., Huang, M. H., and Cao. J. 2008. Differential expression of genes associated with cell proliferation and apoptosis induced by okadaic acid during the transformation process of BALB/c 3T3 cells. Toxicol. In Vitro. 22: 116-27. (Pubitemid 350284644)
4. Aune, T., and Yndestad, M. 1993. Diarrhetic shellfish poisoning. In Algal toxins in seafood and drinking water, ed. I. R. Falconer, 87-104. New York: Academic Press.
5. Avlasevich, S. L., Bryce, S. M., Cairns, S. E., and Dertinger, S. D. 2006. In vitro micronu-cleus scoring by flow cytometry: Differential staining of micronuclei versus apoptotic and necrotic chromatin enhances assay reliabil-ity. Environ. Mol. Mutagen. 47: 56-66. (Pubitemid 43238025)
6. Bialojan, C., and Takai, A. 1988. Inhibitory effect of marine sponge toxin, okadaic acid, on protein phosphatases. Specificity and kinetics. Biochem.J. 256: 283-90.
7. Cabado, A. G., Leira, F., Vieytes, M. R., Vieites, J. M., and Botana, L. M. 2004. Cytoskeletal disruption isthe keyfactorthattriggersapop-tosis in okadaic acid-treated neuroblastoma cells.Arch. Toxicol. 78: 74-85. (Pubitemid 38316662)
8. Carvalho, P. S., Moukha, S. Matias, W. G., and Creppy, E. E. 2006. Comparative study of domoic acid and okadaic acid induced-chromosomal abnormalities in the Caco-2 cell line. Int. J. Environ. Res. Public Health 3: 4-10. (Pubitemid 350002707)
9. Chin, L. S., Singh, S. K., Wang, Q., and Murray, S. F. 2000 Identification of okadaic-acid-induced genes by mRNA differential display in glioma cells. J. Biomed. Sci. 7: 152-59. (Pubitemid 30180159)
10. Colognato, R., Coppedè, F., Ponti, J., Sabbioni, E., and Migliore, L. 2007. Genotoxicity induced by arsenic compounds in periph-eral human lymphocytes analysed by cytokinesis-block micronucleus assay. Mutagenesis 22: 255-61. (Pubitemid 47216614)
11. Cordier, S., Monfort, C., Miossec, L., Richarson, S., and Belin, C. 2000. Ecological analysis of digestive cancer mortality related to contamination by diarrhetic shellfish poisoning toxins along the coasts of France. Environ. Res. 84: 145-50.
12. Creppy, E. E., Traoré, A., Baudrimont, I., Cascante, M., and Carratú, M. R. 2002. Recent advances in the study of epigenetic effects induced by the phycotoxin okadaic acid. Toxicology 182: 433-39. (Pubitemid 36015733)
13. Davis, M. A., Chang, S. H., and Trump, B. F. 1996. Differential sensitivity of normal and H-ras oncogene-transformed rat kid-ney epithelial cells to okadaic acid-induced apoptosis. Toxicol. Appl. Pharmacol. 141: 93-01.
14. Dionysiou, D. 2010. Overview: Harmful algal blooms and natural toxins in fresh and marine waters\-Exposure, occurrence, detection, toxicity, control, managementand policy. Toxicon 55: 907-8.
15. Fenech, M. 2007. Cytokinesis-block micronu-cleus cytome assay. Nat Protocol. 2: 1084-1104.
16. Fernández-Capetillo, O., Lee,A., Nussenzweig, M., and Nussenzweig, A. 2004. H2AX: The histone guardian of the genome. DNA Repair 3: 959-67. (Pubitemid 38997938)
17. Fessard, V., Grosse, Y., Pfohl-Leszkowicz A., and Puiseux-Dao, S. 1996. Okadaic acid treatment induces DNA adduct formation in BHK21 C13 fibroblasts and HESV ker-atinocytes. Mutat. Res. 361: 133-41. (Pubitemid 26420079)
18. Franchini, A., Malagoli, D., and Ottaviani, E. 2010. Targets and effects of yessotoxin, okadaic acid and palytoxin: A differential review. Mar. Drugs 8: 658-77.
19. Fujiki, H., Suganuma, M., Suguri, H., Yoshizawa, S., Takagi, K., Uda, N., Wakamatsu, K., Yamada, K., Murata, M., Yasumoto, T., and Sugimura, T. 1988. Diarrhetic shellfish toxin, dinophysistoxin-1, is a potent tumor promoter on mouse skin. Jpn. J. Cancer Res. 79: 1089-93.
20. Fujiki, H., Suganuma, M., Nishiwaki, S., Yoshizawa, S., Yatsunami, J., Matsushima, R., Furuya, H., Okabe, S., Matsunaga, S., and Sugimura, T. 1992. Specific mechanis-tic aspects of animal tumor promoters: The okadaic acid pathway Prog. Clin. Biol. Res. 374:337-50.
21. Ghosh, S., Paweletz, N., and Schroeter, D. 1992. Effects of okadaic acid on mitotic HeLa cells.J. Cell Sci. 103: 117-24.
22. Gotoh, E., Asakawa, Y., and Kosaka, H. 1995. Inhibition of protein serine/threonine phosphatases directly induces premature chromosome condensation in mam-malian somatic cells. Biomed. Res. 16: 63-68.
23. He, J., Yamada, K., Zou, L.-B., and Nabeshima., T. 2001. Spatial memory deficit and neu-rodegeneration induced by the direct injec-tion of okadaic acid into the hippocampus in rats.J. Neural. Transm. 108: 1435-43. (Pubitemid 34020447)
24. Ito, E., Yasumoto, T., Takai, A., Imanishi, S., and Harada, K. 2002. Investigation of the distri-bution and excretion of okadaic acid in mice using immunostaining method. Toxicon 40: 159-65. (Pubitemid 33016545)
25. Knasmüller, S., Mersch-Sundermann, V., Kevekordes, S., Darroudi, F., Huber, W. W., Hoelzl, C., Bichler, J., and Majer, B. J. 2004. Use of human-derived liver cell lines for the detection of environmental and dietary genotoxicants, current state of knowledge. Toxicology 198: 315-28. (Pubitemid 38618063)
26. Laffon, B., Pásaro, E., and Méndez, J. 2002. DNA damage and repair in human leuko-cytes exposed to styrene-7,8-oxide mea-sured by the comet assay. Toxicol. Lett. 126: 61-68. (Pubitemid 34003567)
27. Lago, J., Santaclara, F., Vieites, J. M., and Cabado, A. G. 2005. Collapse of mito-chondrial membrane potential and cas-pases activation are early events in okadaic acid-treated Caco-2 cells. Toxicon 46: 579-86. (Pubitemid 41258149)
28. Le Hégarat, L., Puech, L., Fessard, V., Poul, J. M., and Dragacci, S. 2003. Aneugenic potential of okadaic acid revealed by the micronucleus assay combined with the FISH technique in CHO-K1 cells. Mutagenesis 18: 293-98. (Pubitemid 36592424)
29. Le Hégarat, L., Fessard, V., Poul, J.M., Dragacci, S., and Sanders, P. 2004. Marine toxin okadaic acid induces aneuploidy in CHO-K1 cells in presence of rat liver postmito-chondrial fraction, revealed by cytokinesis-block micronucleus assay coupled to FISH. Environ. Toxicol. 19: 123-28. (Pubitemid 38500001)
30. Le Hégarat, L., Jacquin, A.G., Bazin E., and Fessard, V. 2006. Genotoxicity of the marine toxin okadaic acid, in human Caco-2 cells and in mice gut cells. Environ. Toxicol. 21: 55-64. (Pubitemid 43277217)
31. Lerga, A., Richard, C., Delgado, M. D., Cañelles, M., Frade, P., Cuadrado, M. A., and León, J. 1999. Apoptosis and mitotic arrest are two independent effects of the protein phosphatases inhibitor okadaic acid in K562 leukemia cells. Biochem. Biophys. Res. Commun. 260: 256-64. (Pubitemid 29351817)
32. López-Rodas, V., Maneiro, E., Martínez, J., Navarro M., and Costas, E. 2006. Harmful algal blooms, red tides and human health: Diarrheic shellfish poisoning and colorectal cancer. Ann. R. Acad. Nac Farm. 72: 391-408. (Pubitemid 46212653)
33. Matias, W. G., and Creppy, E. E. 1996. Evidence for an enterohepatic circulation with okadaic acid in mice, Toxic. Subst. Mech. 15: 405-14. (Pubitemid 26346592)
34. Matias, W. G., Bonini, M., and Creppy, E. E. 1996. Inhibition of protein synthesis in a cell-free system and Vero cells by okadaic acid, a diarrhetic shellfish toxin. J. Toxicol. Environ. Health 48: 309-17. (Pubitemid 26233075)
35. Morimoto, Y., Ohba, T., Kobayashi, S., and Haneji, T. 1997. The protein phosphatase inhibitors okadaic acid and calyculin A induce apoptosis in human osteoblastic cells. Exp. Cell Res. 230: 181-86. (Pubitemid 27190638)
36. Nakagama, H., Kaneko, S., Shima, H., Inamori, H., Fukuda, H., Kominami, R., Sugimura, T., and Nagao, M. 1997. Induction of minisatel-lite mutation in NIH 3T3 cells by treatment with the tumor promoter okadaic acid. Proc. Natl. Acad. Sci. USA 94: 10813-16. (Pubitemid 27430817)
37. Nüsse, M., Beisker, W., Kramer, J., Miller, B. M., Schreiber, G. A., Viaggi, S., Weller, E. M., and Wessels, J. M. 1994. Measurement of micronuclei by flow cytometry. Methods Cell Biol. 42: 149-58.
38. Nüsse, M., and Marx, K. 1997. Flow cytomet-ric analysis of micronuclei in cell cultures and human lymphocytes: Advantages and disadvantages. Mutat. Res. 392: 109-15. (Pubitemid 27327012)
39. Nuydens, R., de Jong, M., Van Den Kieboom, G., Heers, C., Dispersyn, G., Cornelissen, F., Nuyens, R., Borgers, M., and Geerts, H. 1998. Okadaic acid-induced apopto-sis in neuronal cells: Evidence for an abortive mitoticattempt.J. Neurochem. 70: 1124-33. (Pubitemid 28113646)
40. Pérez Machado, G., González Borroto, J.I., Castañedo, N., Creus, A., and Marcos, R. 2004. In vitro genotoxicity testing of the furylethylene derivative UC-245 in human cells. Mutagenesis 19: 75-80. (Pubitemid 38088398)
41. Pilch, D. R., Sedelnikova, O. A., Redon, C., Celeste, A., Nussenzweig, A., and Bonner, W. M. 2003. Characteristics of gamma-H2AX foci at DNA double-strand breaks sites. Biochem. Cell Biol. 81: 123-29. (Pubitemid 37076234)
42. Redon, C., Pilch, D. R., and Bonner, W. M. 2006. Genetic analysis of Saccharomyces cerevisiae H2A serine 129 mutant sug-gests a functional relationship between H2A and the sister-chromatid cohesion part-ners Csm3-Tof1 for the repair of topoiso-merase I-induced DNA damage. Genetics 172: 67-76. (Pubitemid 43345473)
43. Rogakou, E. P., Pilch, D. R., Orr, A. H., Ivanova, V. S., and Bonner, W. M. 1998. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139.J. Biol. Chem. 273: 5858-68. (Pubitemid 28124064)
44. Rogers, C. G., Héroux-Metcalf, C., and Langlois, I. 1994. Evaluation of cytotoxicity and genotoxicity of okadaic acid, a non-phorbol ester type tumour promoter, in V79 Chinese hamster lung cells. Toxicol. In Vitro. 8: 269-76. (Pubitemid 24184196)
45. Roman, D., Locher, F., Suter, W., Cordier, A., and Bobadilla, M. 1998. Evaluation of a new procedure for the flow cytometric analysis of in vitro, chemically induced micronuclei in V79 cells. Environ. Mol. Mutagen. 32: 387-96. (Pubitemid 29019022)
46. Rouse, J., and Jackson, S. P. 2002. Interfaces between the detection, signaling, and repair of DNA damage. Science 297: 547-51. (Pubitemid 34815330)
47. Sakai, A., and Fujiki, H. 1991. Promotion of BALB/3T3 cell transformation by the okadaic acid class of tumor promoters, okadaic acid and dinophysistoxin-1. Jpn. J. CancerRes. 82: 518-23.
48. Schuler, M., Rupa, D. S., and Eastmond, D. A. 1997. A critical evaluation of centromeric labeling to distinguish micronuclei induced by chromosomal loss and breakage in vitro. Mutat. Res. 392: 81-95. (Pubitemid 27327010)
49. Souid-Mensi, G., Moukha, S., Mobio, T.A., Maaroufi, K., and Creppy, E. E. 2008. The cytotoxicity and genotoxicity of okadaic acid are cell-line dependent. Toxicon 51: 1338-44.
50. Suganuma, M., Fujiki, H., Suguiri, H., Yoshizwa, S., Hirota, M., Nakayasu, M., Ojika, M., Wakamatsu, K., Yamada, K., and Sugimura, T. 1988. Okadaic acid, an additional non-phorbol-12-tetradecanoate-13-acetate type tumor promoter. Proc. Natl. Acad. Sci. USA 85: 1768-71. (Pubitemid 24315763)
51. Tanaka, T., Halicka, D., Traganos, F., and Darzynkiewicz, Z. 2009. Cytometric anal-ysis of DNA damage, phosphorylation of histone H2AX as a marker of DNA double-strand breaks (DSBs). Methods Mol. Biol. 523: 161-68.
52. Traoré, A., Baudrimont, I., Ambaliou, S., Dano, S. D., and Creppy, E. E. 2001. DNA breaks and cell cycle arrest induced by okadaic acid in Caco-2 cells, a human colonic epithelial cell line.Arch. Toxicol. 75: 110-17. (Pubitemid 32304544)
53. Valdiglesias, V., Méndez, J., Pásaro, E., Cemeli, E., Anderson, D., and Laffon, B. 2010. Assessment of okadaic acid effects on cyto-toxicity, DNA damage and DNA repair in human cells. Mutat. Res. 689: 74-79.
54. Valdiglesias, V., Méndez, J., Pásaro, E., Cemeli, E., Anderson, D., and Laffon, B. 2011a. Induction of oxidative DNA damage by the marine toxin okadaic acid depends on human cell type. Toxicon. 57: 882-888.
55. Valdiglesias, V., Laffon, B., Pásaro, E., and Méndez, J. 2011b. Okadaic acid induces morphological changes, apopto-sis and cell cycle alterations in different human cell types. J. Environ. Monit. DOI:10.1039/C0EM00771D.
56. Vale, C., and Botana, L. M. 2008. Marine toxins and the cytoskeleton, okadaic acid and dinophysistoxins. FEBS J. 275: 6060-66.
57. Van Dolah, F. M., and Ramsdell, J. S. 1992. Okadaic acid inhibits a protein phosphatase activity involved in formation of the mitotic spindle of GH4 rat pituitary cells. J. Cell Physiol. 152: 190-98.
58. Viaggi, S., Braselmann, H., and Nüsse, M. 1995. Flow cytometric analysis of micronu-clei in the CD2+/- subpopulation of human lymphocytes enriched by mag-netic separation. Int. J. Radiat. Biol. 67: 193-202.
59. Yasumoto, T., Oshima, Y., and Yamaguchi, M. 1978. Occurrence of a new type of shell-fish poisoning in the Tohoku District. Nippon Suisan Gakkaishi. 44: 1249-55.
60. Zhou, B. B., and Elledge, S. J. 2000. The DNA damage response: Putting checkpoints in perspective. Nature 408: 433-39.