Evaluation of a new procedure for the flow cytometric analysis of in vitro, chemically induced micronuclei in V79 cells

Environmental and Molecular Mutagenesis

Volumn 32, Issue 4, 1998, Pages 387-396

  Roman, Danielle ^a   Locher, Franziska ^a   Suter, Willi ^a   Cordier, André ^a   Bobadilla, Maria ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Flow cytometry; In vitro; Micronuclei; Micronucleus test; V79 cells

Indexed keywords

MUTAGENIC AGENT;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE; MICRONUCLEUS; MICROSCOPY; MUTAGEN TESTING; NONHUMAN;

ANIMALS; BLEOMYCIN; CELL LINE; CRICETINAE; CRICETULUS; CYCLOPHOSPHAMIDE; ETHYL METHANESULFONATE; EVALUATION STUDIES; FLOW CYTOMETRY; MICRONUCLEUS TESTS; MUTAGENS; VINBLASTINE; VINCRISTINE;

EID: 0032433849     PISSN: 08936692     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2280(1998)32:4<387::AID-EM13>3.0.CO;2-Y     Document Type: Article

References (27)

1. Beisker W. 1994. A new combined integral-light and slit-scan data analysis system (DAS) for flow cytometry. Comput Methods Programs Biomed 42:15-26.
2. Bertsche U. 1985. Micronucleus induction in mammalian cell cultures treated with ionizing radiations. Radiât Environ Biophys 24:27-44.
3. Countryman PI, Heddle JA. 1976. The production of micronuclei from chromosome aberrations in irradiated cultures of human lymphocytes. Mutat Res 41:321-332.
4. Fenech M, Jarvis LR, Morley AA. 1988. Preliminary studies on scoring micronuclei by computerized image analysis. Mutât Res 203:3338.
5. Fritauff W, Pötter-Locher F, Cordier A, Suter W. 1998. Automatic analysis of the in vitro micronucleus test on V79 cells. Mutât Res 413:5768.
6. Hayashi M, Norppa H, Sofuni T, Ishidate M. 1990. Automation of mouse micronucleus test by flow cytometry and image analysis. Cytometry Suppl 4:35.
7. Heddle JA, Carrano AV. 1977. The DNA content of micronuclei induced in mouse bone marrow by γ-irradiation: Evidence that micronuclei arise from acentric chromosomal fragments. Mutat Res 44:63-69.
8. Heddle JA, Benz RD, Countryman PI. 1978. Measurement of chromosomal breakage in cultured cells by the micronucleus technique. In: Evans HJ, Lloyd DC, eds. Mutagen-induced Chromosome Damage in Man. Edinburgh: Edinburgh University Press, pp 191-200.
9. Heddle JA, Hite M, Kirkhart B, Mavournin K, MacGregor JT, Newell GW, Salamone MF. 1983. The induction of micronuclei as a measure of genotoxicity. A report of the U.S. Environmental Protection Agency GeneTox Program. Mutat Res 123:61-118.
10. Heddle JA, Cimino MC, Hayashi M, Romagna F, Shelby MD, Tucker JD, Vanparys Ph, MacGregor JT. 1991. Micronuclei as an index of cytogenetic damage: Past, present and future. Environ Mol Mutagen 18:277-291.
11. Matsuoka A, Yamazaki N, Suzuki T, Hayashi M, Sofuni T. 1993. Evaluation of the micronucleus test using a Chinese hamster cell line as an alternative to the conventional in vitro chromosomal aberration test. Mutât Res 272:223-236.
12. Mavoumin KH, Blakey DH, Cimino MC, Salamone MF, Heddle JA. 1990. The in vivo micronucleus assay in mammalian bone marrow and peripheral blood. A report of the U.S. Environmental Protection Agency Gen-Tox Program. Mutat Res 239:29-80.
13. Miller BM, Nüsse M. 1993. Analysis of micronuclei induced by 2-chlorobenzylidene malonitrile (CS) using fluorescence in situ hybridization with telomeric and centromeric DNA probes, and flow cytometry. Mutagenesis 8:35-41.
14. Miller BM, Pujadas E, Gocke E. 1995. Evaluation of the micronucleus test in vitro using Chinese hamster cells: Results of four chemicals weakly positive in the in vivo micronucleus test. Environ Mol Mutagen 26:240-247.
15. Miller B, Albertini S, Locher F, Thybaud V, Lorge E. 1997. Comparative evaluation of the in vitro micronucleus test and the in vitro chromosome aberration test: Industrial experience. Mutât Res 392:4759.
16. Nüsse M, Kramer J. 1984. Flow cytometric analysis of micronuclei found in cells after irradiation. Cytometry 5:20-25.
17. Nüsse M, Krämer M. 1987. Antikinetochore antibodies and flow karyotyping: New techniques to detect aneuploidy in mammalian cells induced by ionizing radiation and chemicals. Mol Toxicol 1:393405.
18. Nüsse M, Beisker W, Kramer J, Miller BM, Schreiber GA, Viaggi S, Weller EM, Wessels J. 1984. Measurement of micronuclei by flow cytometry. In: Darzynkiewicz Z, Robinson JP, Crissman HA, eds. Methods in Cell Biology. Vol. 42, Flow Cytometry, 2nd Ed., Part B. San Diego: Academic Press, pp 149-158.
19. Nüsse M, Kramer J, Miller BM. 1992a. Factors influencing the DNA content of radiation-induced micronuclei. Int J Radiât Biol 62:587602.
20. Nüsse M, Recknagel S, Beisker W. 1992b. Micronuclei induced by 2-chlorobenzylidene malonitrile contain single chromosomes as demonstrated by the combined use of flow cytometry and immunofluorescent staining with anti-kinetochore antibodies. Mutagenesis 7:57-67.
21. Ray VA, Kier LD, Kannan KL, Haas RT, Auletta AE, Wassom JS, Nesnow S, Waters MD. 1987. An approach to identifying specialised batteries of bioassays for specific classes of chemicals: Class analysis using mutagenicity and carcinogenicity relationships and phylogenetic concordance and discordance patterns. 1. Composition and analysis of the overall data base. A report of Phase II of the U.S. Environmental Protection Agency GeneTox Program. Mutat Res 185:197-241.
22. Romagna F, Staniforth CD. 1989. The automated bone marrow micronucleus test. Mutât Res 213:91-104.
23. Schneider BM, Wiirgler FE, Romagna F. 1995. Distinct area distribution differences of micronuclei induced by clastogenic and aneuploido-genie chemicals in the bone marrow of the CD-1 mouse. Mutat Res 334:81-89.
24. Schreiber GA, Beisker W, Bauchinger M, Nüsse M. 1992. Multiparametric flow cytometric analysis of radiation-induced micronuclei in mammalian cell cultures. Cytometry 13:90-102.
25. Tales AD, van Welie M, Ploem JJ. 1990. The present state of the automated micronucleus test for lymphocytes. Int J Radiât Biol 58:813825.
26. Viaggi S, Braselmann H, Nüsse M. 1995. Flow cytometric analysis of micronuclei in the CD2± subpopulation of human lymphocytes enriched by magnetic separation. Int J Radiât Biol 67:193-202.
27. Wessels J, Nüsse M. 1995. Row cytometric detection of micronuclei by combined staining of DNA and membranes. Cytometry 19:201208.