Human Telomere POT1-TPP1 Complex and Its Role in Telomerase Activity Regulation

Methods in Molecular Biology

Volumn 735, Issue , 2011, Pages 173-187

  Wang, Feng ^b   Lei, Ming ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b NONE

Author keywords

In vitro telomerase activity; POT1; Protein purification; Telomerase; Telomere; Telomeric proteins; TPP1

Indexed keywords

AMINOPEPTIDASE; DIPEPTIDYL PEPTIDASE; POT1 PROTEIN, HUMAN; SERINE PROTEINASE; TELOMERASE; TELOMERE BINDING PROTEIN; TRIPEPTIDYL PEPTIDASE I; TRIPEPTIDYL-PEPTIDASE 1;

ARTICLE; CHEMISTRY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM;

AMINOPEPTIDASES; DIPEPTIDYL-PEPTIDASES AND TRIPEPTIDYL-PEPTIDASES; HUMANS; SERINE PROTEASES; TELOMERASE; TELOMERE-BINDING PROTEINS;

EID: 79960993207     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-092-8_17     Document Type: Chapter

References (19)

1. Blackburn, E. H. (2001) Switching and signaling at the telomere, Cell 106, 661–673.
2. Cech, T. R. (2004) Beginning to understand the end of the chromosome, Cell 116, 273–279.
3. de Lange, T. (2005) Shelterin: the protein complex that shapes and safeguards human telomeres, Genes Dev 19, 2100–2110.
4. Zhong, Z., Shiue, L., Kaplan, S., and de Lange, T. (1992) A mammalian factor that binds telomeric TTAGGG repeats in vitro, Mol Cell Biol 12, 4834–4843.
5. Bilaud, T., Brun, C., Ancelin, K., Koering, C. E., Laroche, T., and Gilson, E. (1997) Telomeric localization of TRF2, a novel human telobox protein, Nat Genet 17, 236–239.
6. Broccoli, D., Smogorzewska, A., Chong, L., and de Lange, T. (1997) Human telomeres contain two distinct Myb-related proteins, TRF1 and TRF2, Nat Genet 17, 231–235.
7. Baumann, P., and Cech, T. R. (2001) Pot1, the putative telomere end-binding protein in fission yeast and humans, Science 292, 1171–1175.
8. Baumann, P., Podell, E., and Cech, T. R. (2002) Human Pot1 (protection of telomeres) protein: cytolocalization, gene structure, and alternative splicing, Mol Cell Biol 22, 8079–8087.
9. Tani, A., and Murata, M. (2005) Alternative splicing of Pot1 (Protection of telomere)-like genes in Arabidopsis thaliana, Genes Genet Syst 80, 41–48.
10. Wei, C., and Price, C. M. (2004) Cell cycle localization, dimerization, and binding domain architecture of the telomere protein cPot1, Mol Cell Biol 24, 2091–2102.
11. Wu, L., Multani, A. S., He, H., Cosme Blanco, W., Deng, Y., Deng, J. M., Bachilo, O., Pathak, S., Tahara, H., Bailey, S. M., Behringer, R. R., and Chang, S. (2006) Pot1 deficiency initiates DNA damage checkpoint activation and aberrant homologous recombination at telomeres, Cell 126, 49–62.
12. Hockemeyer, D., Daniels, J. P., Takai, H., and de Lange, T. (2006) Recent expansion of the telomeric complex in rodents: Two distinct POT1 proteins protect mouse telomeres, Cell 126, 63–77.
13. Lei, M., Podell, E. R., Baumann, P., and Cech, T. R. (2003) DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA, Nature 426, 198–203.
14. Lei, M., Podell, E. R., and Cech, T. R. (2004) Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection, Nat Struct Mol Biol 11, 1223–1229.
15. Houghtaling, B. R., Cuttonaro, L., Chang, W., and Smith, S. (2004) A dynamic molecular link between the telomere length regulator TRF1 and the chromosome end protector TRF2, Curr Biol 14, 1621–1631.
16. Liu, D., Safari, A., O’Connor, M. S., Chan, D. W., Laegeler, A., Qin, J., and Songyang, Z. (2004) PTOP interacts with POT1 and regulates its localization to telomeres, Nat Cell Biol 6, 673–680.
17. Ye, J. Z., Hockemeyer, D., Krutchinsky, A. N., Loayza, D., Hooper, S. M., Chait, B. T., and de Lange, T. (2004) POT1-interacting protein PIP1: a telomere length regulator that recruits POT1 to the TIN2/TRF1 complex, Genes Dev 18, 1649–1654.
18. Wang, F., Podell, E. R., Zaug, A. J., Yang, Y., Baciu, P., Cech, T. R., and Lei, M. (2007) The POT1-TPP1 telomere complex is a telomerase processivity factor, Nature 445, 506–510.
19. Chen, J. L., and Greider, C. W. (2003) Determinants in mammalian telomerase RNA that mediate enzyme processivity and crossspecies incompatibility, EMBO J 22, 304–314.