Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR

Virology Journal

Volumn 8, Issue , 2011, Pages

  Zhang, Ni ^a   Liu, Zhengwen ^a   Han, Qunying ^a   Qiu, Jianming ^b   Chen, Jinghong ^a   Zhang, Guoyu ^a   Li, Zhu ^a   Lou, Sai ^a   Li, Na ^a  

^a XI'AN JIAOTONG UNIVERSITY   (China)

^b UNIVERSITY OF KANSAS SCHOOL OF MEDICINE   (United States)

Author keywords

Bovine viral diarrhea virus type 1; Cell culture; cRNA standard; Quantitation; SYBR Green I RT PCR

Indexed keywords

DYE; VIRUS RNA; ORGANIC COMPOUND; SYBR GREEN I;

ANIMAL CELL; ARTICLE; BOVINE DIARRHEA VIRUS; CELL CULTURE; CONTROLLED STUDY; IN VITRO STUDY; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; ONE STEP SYBR GREEN I RT PCR ASSAY; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; ANIMAL; CATTLE; COMPARATIVE STUDY; CULTURE TECHNIQUE; EVALUATION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; STAINING; VIRUS LOAD;

ANIMALIA; BOS; BOVINE VIRAL DIARRHEA VIRUS 1; HEPATITIS C VIRUS;

ANIMALS; CATTLE; CELL CULTURE TECHNIQUES; DIARRHEA VIRUS 1, BOVINE VIRAL; ORGANIC CHEMICALS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING; VIRAL LOAD;

EID: 79960656595     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/1743-422X-8-374     Document Type: Article

References (28)

1. Bovine viral diarrhea virus types 1 and 2 antibody response in calves receiving modified live virus or inactivated vaccines. Fulton RW, Burge LJ, Vaccine 2001 19 264 274 (Pubitemid 30621520)
2. Segregation of bovine viral diarrhea virus into genotypes. Ridpath JF, Bolin SR, Dubovi EJ, Virology 1994 205 66 74 10.1006/viro.1994.1620 7975238 (Pubitemid 24360591)
3. Flaviviridae: the viruses and their replication. Rice CM, Fields virology Lippincott-Raven Publishers, Philadelphia, Fields BN, Knipe DM, Howley PM, 3 1996 1 931 960
4. Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Colett MS, Larson R, Gold C, Strick D, Anderson DK, Purchio AF, Virology 1988 165 191 199 10.1016/0042-6822(88)90672-1 2838957
5. Genetic organization and diversity of the hepatitis C virus. Choo QL, Richman KH, Han JH, Berger K, Lee C, Dong C, Gallegos C, Coit D, Medina-Selby R, Barr PJ, Weiner AJ, Bradleyt DW, Kuo G, Houghton M, Proc Natl Acad Sci USA 1991 88 2451 2455 10.1073/pnas.88.6.2451 1848704 (Pubitemid 21916435)
6. Synergistic in vitro interactions between alpha interferon and RBV against bovine viral diarrhea virus and yellow fever virus as surrogate models of hepatitis C virus replication. Buckwold VE, Wei J, Wenzel-Mathers M, Russell J, Antimicrob Agents Chemother 2003 47 2293 8 10.1128/AAC.47.7.2293-2298.2003 12821481 (Pubitemid 36753585)
7. Bovine viral diarrhea virus as a surrogate model of hepatitis C virus for the evaluation of antiviral agents. Buckwold VE, Beer BE, Donis RO, Antiviral Res 2003 60 1 15 10.1016/S0166-3542(03)00174-8 14516916 (Pubitemid 37153391)
8. Diagnostic assays applied in BVDV control in The Netherlands. Mars MH, Van Maanen C, Prev Vet Med 2005 72 43 48 discussion 215-219 10.1016/j.prevetmed. 2005.08.005 16199104 (Pubitemid 41627320)
9. An antigen-capture ELISA detects pestivirus antigens in blood and tissues of immunotolerant carrier cattle. Shannon AD, Richards SG, Kirkland PD, Moyle A, J Virol Methods 1991 34 1 12 1659578
10. Reverse transcription-polymerase chain reaction on pooled samples to detect bovine viral diarrhea virus by using fresh ear-notch-sample supernatants. Kennedy JA, Mortimer RG, Powers B, J Vet Diagn Invest 2006 18 89 93 10.1177/104063870601800113 16566263 (Pubitemid 43188319)
11. Comparison of RT-PCR assay and virus isolation in cell cultures for the detection of bovine viral diarrhoea virus (BVDV) in field samples. Laamanen UI, Neuvonen EP, Yliviuhkola EM, Veijalainen PM, Res Vet Sci 1997 63 199 203 10.1016/S0034-5288(97)90020-5 9491443 (Pubitemid 128520039)
12. Comparison of virus isolation and reverse transcription polymerase chain reaction assay for detection of bovine viral diarrhea virus in bulk milk tank samples. Renshaw RW, Ray R, Dubovi EJ, J Vet Diagn Invest 2000 12 184 186 10.1177/104063870001200219 10730955
13. Typing of bovine viral diarrhea viruses directly from blood of persistently infected cattle by multiplex PCR. Gilbert SA, Burton KM, Prins SE, Deregt D, J Clin Microbiol 1999 37 2020 2023 10325368 (Pubitemid 29235267)
14. Comparison of five diagnostic methods for detecting bovine viral diarrhea virus infection in calves. Hilbe M, Stalder H, Peterhans E, Haessig M, Nussbaumer M, Egli C, Schelp C, Zlinszky K, Ehrensperger F, J Vet Diagn Invest 2007 19 28 34 10.1177/104063870701900105 17459829 (Pubitemid 351201918)
15. Fluorogenic RT-PCR assay (TaqMan) for detection and classification of bovine viral diarrhea virus. Bhudevi B, Weinstock D, Vet Microbiol 2001 83 1 10 10.1016/S0378-1135(01)00390-X 11524161 (Pubitemid 32778456)
16. Detection of bovine viral diarrhea virus in formalin fixed paraffin embedded tissue sections by real time RT-PCR (Taqman). Bhudevi B, Weinstock D, J Virol Methods 2003 109 25 30 10.1016/S0166-0934(03)00040-5 12668264 (Pubitemid 36355885)
17. Detection of bovine viral diarrhea virus by TaqMan reverse transcription polymerase chain reaction. Mahlum CE, Haugerud S, Shivers JL, Rossow KD, Goyal SM, Collins JE, Faaberg KS, J Vet Diagn Invest 2002 14 120 125 10.1177/104063870201400205 11939332 (Pubitemid 135746905)
18. Cross-platform comparison of SYBR Green real-time PCR with TaqMan PCR, microarrays and other gene expression measurement technologies evaluated in the MicroArray Quality Control (MAQC) study. Arikawa E, Sun Y, Wang J, Zhou Q, Ning B, Dial SL, Guo L, Yang J, BMC Genomics 2008 9 328 10.1186/1471-2164-9-328 18620571
19. Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods. Schmittgen TD, Zakrajsek BA, Mills AG, Gorn V, Singer MJ, Reed MW, Anal Biochem 2000 285 194 204 10.1006/abio.2000.4753 11017702
20. Real-time RT-PCR detection of Bovine Viral Diarrhoea virus in whole blood using an external RNA reference. Young NJ, Thomas CJ, Collins ME, Brownlie J, J Virol Methods 2006 138 218 222 10.1016/j.jviromet.2006.08.008 17030066 (Pubitemid 44633462)
21. National standards of the P.R.C., diagnostic techniques for bovine viral diarrhea/mucosal disease. Standardization Administration of the People's Republic of China, First Editing Room of Standards Press of China, Collection of National Animal Health Standardization Standards Press of China, Beijing 2004 127 133
22. A simple method of estimating fifty percent endpoints. Reed LJ, Muench AH, Am J Hyg 1938 27 493 497
23. A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction. Fronhoffs S, Totzke G, Stier S, Wernert N, Rothe M, Brüning T, Koch B, Sachinidis A, Vetter H, Ko Y, Mol Cell Probes 2002 16 99 110 10.1006/mcpr.2002.0405 12030760 (Pubitemid 34712934)
24. Detection of minimal residual disease in hematologic malignancies by real-time quantitative PCR: principles, approaches, and laboratory aspects. van der Velden VH, Hochhaus A, Cazzaniga G, Szczepanski T, Gabert J, van Dongen JJ, Leukemia 2003 17 1013 1034 10.1038/sj.leu.2402922 12764363 (Pubitemid 36722231)
25. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Ririe KM, Rasmussen RP, Wittwer CT, Anal Biochem 1997 245 154 160 10.1006/abio.1996.9916 9056205 (Pubitemid 27082244)
26. A SYBR Green RT-PCR assay in single tube to detect human and bovine noroviruses and control for inhibition. Scipioni A, Mauroy A, Ziant D, Saegerman C, Thiry E, Virol J 2008 5 94 10.1186/1743-422X-5-94 18702817
27. The detection of bovine viral diarrhea virus in bulk milk samples by the use of a single tube RT-PCR. Drew TW, Yapp F, Paton DJ, Vet Microbiol 1999 64 145 154 10.1016/S0378-1135(98)00266-1 10028169 (Pubitemid 29073592)
28. Single-tube single-enzyme reverse transcriptase PCR assay for detection of bovine viral diarrhea virus in pooled bovine serum. Weinstock D, Bhudevi B, Castro AE, J Clin Microbiol 2001 39 343 346 10.1128/JCM.39.1.343-346.2001 11136796 (Pubitemid 32059616)