A novel, high stringency selection system allows screening of few clones for high protein expression

Journal of Biotechnology

Volumn 128, Issue 2, 2007, Pages 237-245

  van Blokland, H J M ^a   Kwaks, T H J ^a   Sewalt, R G A B ^a   Verhees, J A ^a   Klaren, V N A ^a   Siersma, T K ^a   Korse, J W M ^a   Teunissen, N C ^a   Botschuijver, S ^a   van Mer, C ^a   Man, S Y ^a   Otte, A P ^a,b,c  

^a ChromaGenics   (Netherlands)

^b CRUCELL HOLLAND BV   (Netherlands)

^c UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Anti repressor elements; Mammalian cell lines; Selection system

Indexed keywords

ATTENUATION; BIOMARKERS; GENES; NATURAL FREQUENCIES; PROTEINS; RNA;

ANTI REPRESSOR ELEMENTS; ATTENUATED TRANSLATION INITIATION FREQUENCY; MAMMALIAN CELL LINES; SELECTION SYSTEMS;

CELL CULTURE;

MESSENGER RNA;

ANIMAL CELL; ARTICLE; CELL CLONE; CELL SELECTION; CONTROLLED STUDY; GENE EXPRESSION SYSTEM; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RNA TRANSCRIPTION; START CODON; TRANSLATION INITIATION;

ANIMALS; CELL LINE; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; GENE DOSAGE; GENE EXPRESSION REGULATION; TRANSFECTION;

MAMMALIA;

EID: 33846095340     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.09.023     Document Type: Article

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