Two-year monitoring of Cryptosporidium parvum and Giardia lamblia occurrence in a recreational and drinking water reservoir using standard microscopic and molecular biology techniques

Environmental Monitoring and Assessment

Volumn 179, Issue 1-4, 2011, Pages 163-175

  Helmi, Karim ^a,b   Skraber, Sylvain ^a   Burnet, Jean Baptiste ^a   Leblanc, Laurence ^a   Hoffmann, Lucien ^a   Cauchie, Henry Michel ^a  

^a LUXEMBOURG INSTITUTE OF SCIENCE AND TECHNOLOGY   (Luxembourg)

^b Veolia Environnement Recherche et Innovation   (France)

Author keywords

Cryptosporidium parvum; Faecal indicator bacteria; Giardia lamblia; Microscopy; qPCR; Water

Indexed keywords

CRYPTOSPORIDIUM PARVUM; DISTRIBUTION NETWORK; FAECAL INDICATORS; GIARDIA LAMBLIA; LUXEMBOURG; MAXIMAL RISK; OOCYSTS; PARASITE INFECTION; PARASITE-; PATHOGENIC PROTOZOA; PRESENCE/ABSENCE; QPCR; RAIN EVENTS; WATER SAMPLES; WATER TURBIDITY;

MOLECULAR BIOLOGY; PLANTS (BOTANY); POLYMERASE CHAIN REACTION; POTABLE WATER; PROTOZOA; RISK PERCEPTION; TURBIDITY; WATER ANALYSIS; WATER TREATMENT;

RESERVOIRS (WATER);

DRINKING WATER;

BACTERIUM; BIOLOGY; BIOMONITORING; DRINKING WATER; MICROSCOPY; MOLECULAR ANALYSIS; PARASITE; POLYMERASE CHAIN REACTION; PROTOZOAN; RESERVOIR; TURBIDITY;

ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; ENVIRONMENTAL MONITORING; GIARDIA LAMBLIA; IMMUNOFLUORESCENCE MICROSCOPY; INFECTION RISK; NONHUMAN; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; RECREATION; RISK ASSESSMENT; TURBIDITY; WATER ANALYSIS; WATER CONTAMINATION; WATER SAMPLING; WATER SUPPLY;

CRYPTOSPORIDIUM PARVUM; ENVIRONMENTAL MONITORING; FRESH WATER; GIARDIA LAMBLIA; MICROSCOPY, FLUORESCENCE; OOCYSTS; RISK ASSESSMENT; WATER POLLUTION; WATER SUPPLY;

CRYPTOSPORIDIUM PARVUM; GIARDIA INTESTINALIS; PROTOZOA;

EID: 79960435767     PISSN: 01676369     EISSN: 15732959     Source Type: Journal    
DOI: 10.1007/s10661-010-1726-7     Document Type: Article

References (48)

1. I Bertrand J Schwartzbrod 2007 Detection and genotyping of Giardia duodenalis in wastewater: Relation between assemblages and faecal contamination origin Water Research 41 16 3675 3682 10.1016/j.watres.2007.02.043 1:CAS:528:DC%2BD2sXosFals7o%3D (Pubitemid 47176101)
2. WQ Betancourt JB Rose 2004 Drinking water treatment processes for removal of Cryptosporidium and Giardia Veterinary Parasitology 126 1-2 219 234 10.1016/j.vetpar.2004.09.002 1:CAS:528:DC%2BD2cXhtVakt7bP (Pubitemid 39572944)
3. R Briancesco L Bonadonna 2005 An Italian study on Cryptosporidium and Giardia in wastewater, fresh water and treated water Environmental Monitoring and Assessment 104 1-3 445 457 10.1007/s10661-005-2282-4 1:CAS:528: DC%2BD2MXjt1GqsL4%3D (Pubitemid 40613378)
4. JD Brookes J Antenucci M Hipsey MD Burch NJ Ashbolt C Ferguson 2004 Fate and transport of pathogens in lakes and reservoirs Environment International 30 5 741 759 10.1016/j.envint.2003.11.006 (Pubitemid 39022754)
5. JD Brookes MR Hipsey MD Burch RH Regel LG Linden CM Ferguson, et al. 2005 Relative value of surrogate indicators for detecting pathogens in lakes and reservoirs Environmental Sience and Technology 39 22 8614 8621 10.1021/es050821+ 1:CAS:528:DC%2BD2MXhtFWku7jO (Pubitemid 41636126)
6. Castro-Hermida, J. A.; Garcia-Presedo, I.; Gonzalez-Warleta, M.; Mezo, M. (2010). Cryptosporidium and Giardia detection in water bodies of Galicia, Spain. Water Research (in press).
7. CM Davies JA Long M Donald NJ Ashbolt 1995 Survival of fecal microorganisms in marine and freshwater sediments Applied Environmental Microbiology 61 5 1888 1896 1:CAS:528:DyaK2MXlsFyktLo%3D (Pubitemid 26449459)
8. CM Davies C Kaucner D Deere NJ Ashbolt 2003 Recovery and enumeration of Cryptosporidium parvum from animal fecal matrices Applied Environmental Microbiology 69 5 2842 2847 10.1128/AEM.69.5.2842-2847.2003 1:CAS:528: DC%2BD3sXjslOltr0%3D (Pubitemid 36560364)
9. D Dawson 2005 Foodborne protozoan parasites International Journal of Food Microbiology 103 2 207 227 10.1016/j.ijfoodmicro.2004.12.032 (Pubitemid 41080481)
10. AP Dufour O Evans TD Behymer R Cantu 2006 Water ingestion during swimming activities in a pool: A pilot study Journal of Water Health 4 4 425 430 (Pubitemid 44895939)
11. R Fayer 2004 Cryptosporidium: A water-borne zoonotic parasite Veterinary Parasitology 126 1-2 37 56 10.1016/j.vetpar.2004.09.004 (Pubitemid 39572936)
12. R Fayer U Morgan SJ Upton 2000 Epidemiology of Cryptosporidium: Transmission, detection and identification International Journal for Parasitology 30 12-13 1305 1322 10.1016/S0020-7519(00)00135-1 1:STN:280:DC%2BD3MzgsVSqtQ%3D%3D
13. M Fontaine E Guillot 2003 An immunomagnetic separation-real-time PCR method for quantification of Cryptosporidium parvum in water samples Journal of Microbiological Methods 54 1 29 36 10.1016/S0167-7012(03)00005-8 1:CAS:528:DC%2BD3sXjsVWmt7o%3D (Pubitemid 36555792)
14. A Giangaspero R Cirillo V Lacasella A Lonigro M Marangi P Cavallo, et al. 2009 Giardia and Cryptosporidium in inflowing water and harvested shellfish in a lagoon in Southern Italy Parasitology International 58 1 12 17 10.1016/j.parint.2008.07.003
15. RA Guy P Payment UJ Krull PA Horgen 2003 Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage Applied Environmental Microbiology 69 9 5178 5185 10.1128/AEM.69.9.5178-5185. 2003 1:CAS:528:DC%2BD3sXntlSjtLo%3D (Pubitemid 37150736)
16. S Hallier-Soulier E Guillot 2000 Detection of cryptosporidia and Cryptosporidium parvum oocysts in environmental water samples by immunomagnetic separation-polymerase chain reaction Journal of Applied Microbiology 89 1 5 10 10.1046/j.1365-2672.2000.01029.x 1:CAS:528:DC%2BD3cXlsF2hu70%3D (Pubitemid 30602417)
17. ML Hanninen A Horman R Rimhanen-Finne H Vahtera S Malmberg S Herve, et al. 2005 Monitoring of Cryptosporidium and Giardia in the Vantaa river basin, southern Finland International of Hygiene and Environmental Health 208 3 163 171 10.1016/j.ijheh.2005.01.026 (Pubitemid 40592089)
18. K Helmi S Skraber C Gantzer R Willame L Hoffmann HM Cauchie 2008 Interactions of Cryptosporidium parvum, Giardia lamblia, vaccinal poliovirus type 1, and bacteriophages phiX174 and MS2 with a drinking water biofilm and a wastewater biofilm Applied Environmental Microbiology 74 7 2079 2088 10.1128/AEM.02495-07 1:CAS:528:DC%2BD1cXks1anurk%3D (Pubitemid 351522061)
19. A Horman R Rimhanen-Finne L Maunula CH von Bonsdorff N Torvela A Heikinheimo, et al. 2004 Campylobacter spp.; Giardia spp.; Cryptosporidium spp.; noroviruses, and indicator organisms in surface water in southwestern Finland, 2000-2001 Applied Environmental Microbiology 70 1 87 95 10.1128/AEM.70.1.87-95. 2004 (Pubitemid 38090191)
20. KL Jellison HF Hemond DB Schauer 2002 Sources and species of cryptosporidium oocysts in the Wachusett Reservoir watershed Applied Environmental Microbiology 68 2 569 575 10.1128/AEM.68.2.569-575.2002 1:CAS:528:DC%2BD38XhtV2gu70%3D (Pubitemid 34113042)
21. J Jiang KA Alderisio A Singh L Xiao 2005 Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors Applied Environmental Microbiology 71 3 1135 1141 10.1128/AEM.71.3.1135-1141.2005 1:CAS:528:DC%2BD2MXisVOjurc%3D (Pubitemid 40396647)
22. DW Johnson NJ Pieniazek DW Griffin L Misener JB Rose 1995 Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples Applied Environmental Microbiology 61 11 3849 3855 1:CAS:528: DyaK2MXptFGgtr0%3D
23. MW LeChevallier WD Norton RG Lee 1991 Giardia and Cryptosporidium spp. in filtered drinking water supplies Applied Environmental Microbiology 57 9 2617 2621 1:STN:280:DyaK387hslKjtw%3D%3D
24. WA Miller DJ Lewis MD Pereira M Lennox PA Conrad KW Tate, et al. 2008 Farm factors associated with reducing Cryptosporidium loading in storm runoff from dairies Journal of Environmental Quality 37 5 1875 1882 10.2134/jeq2007.0413 1:CAS:528:DC%2BD1cXhtFagu73O
25. C Mons A Dumetre S Gosselin C Galliot L Moulin 2009 Monitoring of Cryptosporidium and Giardia river contamination in Paris area Water Research 43 1 211 217 10.1016/j.watres.2008.10.024 1:CAS:528:DC%2BD1cXhsFCjtrvP
26. D Mueller-Doblies M Giles K Elwin RP Smith FA Clifton-Hadley RM Chalmers 2008 Distribution of Cryptosporidium species in sheep in the UK Veterinary Parasitology 154 3-4 214 219 10.1016/j.vetpar.2008.03.021
27. K Obiri-Danso K Jones 1999 Distribution and seasonality of microbial indicators and thermophilic campylobacters in two freshwater bathing sites on the River Lune in northwest England Journal of Applied Microbiology 87 6 822 832 10.1046/j.1365-2672.1999.00924.x 1:STN:280:DC%2BD3c7ivVaqsA%3D%3D (Pubitemid 30055695)
28. ME Olson J Goh M Phillips N Guselle TA McAllister 1999 Giardia Cyst and Cryptosporidium Oocyst Survival in Water, Soil, and Cattle Feces Journal of Environmental Quality 28 6 1991 1996 10.2134/jeq1999.00472425002800060040x 1:CAS:528:DC%2BD3cXhtFWlug%3D%3D (Pubitemid 30012634)
29. P Payment E Franco 1993 Clostridium perfringens and somatic coliphages as indicators of the efficiency of drinking water treatment for viruses and protozoan cysts Applied Environmental Microbiology 59 8 2418 2424 1:STN:280:DyaK3sznvVChtg%3D%3D (Pubitemid 23222976)
30. SJ Pereira NE Ramirez L Xiao LA Ward 2002 Pathogenesis of human and bovine Cryptosporidium parvum in gnotobiotic pigs Journal of Infectious Disease 186 5 715 718 10.1086/342296 (Pubitemid 34925547)
31. J Quilez E Torres RM Chalmers SJ Hadfield CE Del C Sanchez-Acedo 2008 Cryptosporidium genotypes and subtypes in lambs and goat kids in Spain Applied Environmental Microbiology 74 19 6026 6031 10.1128/AEM.00606-08 1:CAS:528:DC%2BD1cXht1ektb3M
32. NE Ramirez S Sreevatsan 2006 Development of a sensitive detection system for Cryptosporidium in environmental samples Veterinary Parasitology 136 3-4 201 213 10.1016/j.vetpar.2005.11.023
33. RC Rendtorff CJ Holt 1954 The experimental transmission of human intestinal protozoan parasites. IV. Attempts to transmit Endamoeba coli and Giardia lamblia cysts by water American Journal of Hygien 60 3 327 338 1:STN:280:DyaG2M%2FhvVOnsg%3D%3D
34. LJ Robertson B Gjerde 2001 Occurrence of Cryptosporidium oocysts and Giardia cysts in raw waters in Norway Scandinavian Journal of Public Health 29 3 200 207 10.1177/14034948010290030901 1:STN:280:DC%2BD3Mrnt12rsQ%3D%3D (Pubitemid 33731053)
35. LJ Robertson AT Campbell HV Smith 1992 Survival of Cryptosporidium parvum oocysts under various environmental pressures Applied Environmental Microbiology 58 11 3494 3500 1:CAS:528:DyaK3sXjsFOjtA%3D%3D (Pubitemid 24406987)
36. LJ Robertson T Forberg L Hermansen BK Gjerde JO Alvsvag N Langeland 2006 Cryptosporidium parvum infections in Bergen, Norway, during an extensive outbreak of waterborne giardiasis in autumn and winter 2004 Applied Environmental Microbiology 72 3 2218 2220 10.1128/AEM.72.3.2218-2220.2006 1:CAS:528:DC%2BD28XjsFCmtL0%3D
37. M Santin JM Trout L Xiao L Zhou E Greiner R Fayer 2004 Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves Veterinary Parasitology 122 2 103 117 10.1016/j.vetpar.2004.03.020 (Pubitemid 38737304)
38. M Santin JM Trout R Fayer 2008 A longitudinal study of cryptosporidiosis in dairy cattle from birth to 2 years of age Veterinary Parasitology 155 1-2 15 23 10.1016/j.vetpar.2008.04.018
39. FM Schets JH van Wijnen JF Schijven H Schoon AM Roda Husman 2008 Monitoring of waterborne pathogens in surface waters in Amsterdam, The Netherlands, and the potential health risk associated with exposure to Cryptosporidium and Giardia in these waters. Applied Environmental Microbiology 74 7 2069 2078 10.1128/AEM.01609-07 1:CAS:528:DC%2BD1cXks1anurg%3D (Pubitemid 351522060)
40. HV Smith SM Caccio A Tait J McLauchlin RC Thompson 2006 Tools for investigating the environmental transmission of Cryptosporidium and Giardia infections in humans Trends in Parasitology 22 4 160 167 10.1016/j.pt.2006.02. 009
41. P Tallon B Magajna C Lofranco KT Leung 2006 Microbial Indicators Of Faecal Contamination In Water: A Current Perspective Water, Air and Soil Pollution 166 139 166 10.1007/s11270-005-7905-4
42. Teunis, P. F. M.; van der Heijen, O. G.; van der Giessen, J. W. B.; Havelaar, A. H. (1996). Dose response relation in human volunteers for gastro-intestinal pathogens. RIVM report nr.284550002, 1-97.
43. A Touron T Berthe G Gargala M Fournier M Ratajczak P Servais, et al. 2007 Assessment of faecal contamination and the relationship between pathogens and faecal bacterial indicators in an estuarine environment (Seine, France) Marine Pollution Bulletin 54 9 1441 1450 10.1016/j.marpolbul.2007.05.009 1:CAS:528:DC%2BD2sXhtVCmurjN
44. S Tzipori G Widmer 2008 A hundred-year retrospective on cryptosporidiosis Trends in Parasitology 24 4 184 189 10.1016/j.pt.2008.01.002
45. USEPA 1623 (2001). Cryptosporidium and Giardia in Water Filtration/IMS/FA. Protocol, EPA 821-R-01-025. Office of Water, U.S. Environmental Protection Agency, Washington, D.C.
46. JJ Verweij RA Blange K Templeton J Schinkel EA Brienen MA van Rooyen, et al. 2004 Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR Journal of Clinical Microbiology 42 3 1220 1223 10.1128/JCM.42.3.1220-1223.2004 1:CAS:528:DC%2BD2cXivF2ktrs%3D (Pubitemid 38332851)
47. R Willame I Thys V Jacquet M Berbanck HM Cauchie L Hoffmann 2008 Environmental variables involved in the development of bloom-forming cyanobacteria in a meso-eutrophic reservoir Algological Studies 126 227 247 10.1127/1864-1318/2008/0126-0229
48. L Xiao R Fayer 2008 Molecular characterisation of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission International Journal for Parasitology 38 11 1239 1255 10.1016/j.ijpara.2008.03. 006 1:CAS:528:DC%2BD1cXps12ju7Y%3D