Development of a sensitive detection system for Cryptosporidium in environmental samples

Veterinary Parasitology

Volumn 136, Issue 3-4, 2006, Pages 201-213

  Ramirez, Norma E ^a   Sreevatsan, Srinand ^a,b  

^a OHIO STATE UNIVERSITY   (United States)

^b UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Cryptosporidium; Detection; Hybridization; Internal positive control; Real time PCR

Indexed keywords

SODIUM CHLORIDE; SUCROSE;

ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIUM; DNA EXTRACTION; HYBRIDIZATION; NONHUMAN; OOCYST; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PURIFICATION; REAL TIME POLYMERASE CHAIN REACTION; SOIL;

ANIMALS; AREA UNDER CURVE; BASE SEQUENCE; CRYPTOSPORIDIUM; DISEASE RESERVOIRS; DNA PRIMERS; DNA, PROTOZOAN; ENVIRONMENT; FECES; MANURE; OOCYSTS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOIL; TEMPERATURE; WATER;

CRYPTOSPORIDIUM;

EID: 33644622779     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetpar.2005.11.023     Document Type: Article

References (31)

1. R.S. Barwick, H.O. Mohammed, A.B. White, and R.T. Bryan Detection of Cryptosporidium parvum and Cryptosporidium muris in soil samples Biol. Fertile Soils 31 2000 385 390
2. F. Betsou, K. Beaumont, J.M. Sueur, and J. Orfila Construction and evaluation of internal control DNA for PCR amplification of Chlamydia trachomatis DNA from urine samples J. Clin. Microbiol. 41 2003 1274 1276
3. S.M. Caccio Molecular techniques to detect and identify protozoan parasites in the environment Acta Microbiol. Pol. 52 Suppl. 2003 23 34
4. T. Chesnot, and J. Schwartzbrod Quantitative and qualitative comparison of density-based purification methods for detection of Cryptosporidium oocysts in turbid environmental matrices J. Microbiol. Meth. 58 2004 375 386
5. W.L. Current Techniques and laboratory maintenance of Cryptosporidium R. Fayer Cryptsoporidiosis of Man and Animals 1990 CRC Press Boca Raton, FL 41 44
6. R. Fayer Cryptosporidium: a water-borne zoonotic parasite Vet. Parasitol. 126 2004 37 56
7. L.J. Hartman, S.R. Coyne, and D.A. Norwood Development of a novel internal positive control for Taqman based assays Mol. Cell Probes 19 2005 51 59
8. J.A. Higgins, R. Fayer, J.M. Trout, L. Xiao, A.A. Lal, S. Kerby, and M.C. Jenkins Real-time PCR for the detection of Cryptosporidium parvum J. Microbiol. Meth. 47 2001 323 337
9. A. Joachim, E. Eckert, F. Petry, R. Bialek, and A. Daugschies Comparison of viability assays for Cryptosporidium parvum oocysts after disinfection Vet. Parasitol. 111 2003 47 57
10. D.W. Johnson, N.J. Pieniazek, D.W. Griffin, L. Misener, and J.B. Rose Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples Appl. Environ. Microbiol. 61 1995 3849 3855
11. S. Kato, and D.D. Bowman Using flow cytometry to determine the viability of Cryptosporidium parvum oocysts extracted from spiked environmental samples in chambers Parasitol. Res. 88 2002 326 331
12. E. Kuczynska, D.G. Boyer, and D.R. Shelton Comparison of immunofluorescence assay and immunomagnetic electrochemiluminescence in detection of Cryptosporidium parvum oocysts in karst water samples J. Microbiol. Meth. 53 2003 17 26
13. E. Kuczynska, and D.R. Shelton Method for detection and enumeration of Cryptosporidium parvum oocysts in feces, manures, and soils Appl. Environ. Microbiol. 65 1999 2820 2826
14. P.G. Lantz, M. Matsson, T. Wadstrom, and P. Radstrom Removal of PCR inhibitors from human faecal samples through the use of an aqueous two-phase system for sample preparation prior to PCR J. Microbiol. Meth. 28 1997 159 167
15. M.A. Laxer, B.K. Timblin, and R.J. Patel DNA sequences for the specific detection of Cryptosporidium parvum by the polymerase chain reaction Am. J. Trop. Med. Hyg. 45 1991 688 694
16. J.R. Limor, A.A. Lal, and L. Xiao Detection and differentiation of Cryptosporidium parasites that are pathogenic for humans by real-time PCR J. Clin. Microbiol. 40 2002 2335 2338
17. J.L. Mawdsley, A.E. Brooks, and R.J. Merry Movement of the protozoan pathogen Cryptosporidium parvum through three contrasting soil types Biol. Fertil. Soils 21 1996 30 36
18. L. Monteiro, D. Bonnemaison, A. Vekris, K.G. Petry, J. Bonnet, R. Vidal, J. Cabrita, and F. Megraud Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model J. Clin. Microbiol. 35 1997 995 998
19. K.L. Mumy, and R.H. Findlay Convenient determination of DNA extraction efficiency using an external DNA recovery standard and quantitative-competitive PCR J. Microbiol. Meth. 57 2004 259 268
20. C.K. Nielsen, and L.A. Ward Enhanced detection of Cryptosporidium parvum in the acid-fast stain J. Vet. Diagn. Invest. 11 1999 567 569
21. O. Priha, K. Hallamaa, M. Saarela, and L. Raaska Detection of Bacillus cereus group bacteria from cardboard and paper with real-time PCR J. Ind. Microbiol. Biotechnol. 31 2004 161 169
22. P. Radstrom, R. Knutsson, P. Wolffs, M. Lovenklev, and C. Lofstrom Pre-PCR processing: strategies to generate PCR-compatible samples Mol. Biotechnol. 26 2004 133 146
23. U.M. Ryan, P. Monis, H.L. Enemark, I. Sulaiman, B. Samarasinghe, C. Read, R. Buddle, I. Robertson, L. Zhou, R.C. Thompson, and L. Xiao Cryptosporidium suis n. sp. (Apicomplexa: Cryptosporidiidae) in pigs (Sus scrofa) J. Parasitol. 90 2004 769 773
24. S. Sreevatsan, J.B. Bookout, F. Ringpis, V.S. Perumaalla, T.A. Ficht, L.G. Adams, S.D. Hagius, P.H. Elzer, B.J. Bricker, G.K. Kumar, M. Rajasekhar, S. Isloor, and R.R. Barathur A multiplex approach to molecular detection of Brucella abortus and/or Mycobacterium bovis infection in cattle J. Clin. Microbiol. 38 2000 2602 2610
25. I.M. Sulaiman, U.M. Morgan, R.C. Thompson, A.A. Lal, and L. Xiao Phylogenetic relationships of Cryptosporidium parasites based on the 70-kilodalton heat shock protein (HSP70) gene Appl. Environ. Microbiol. 66 2000 2385 2391
26. Y.L. Tsai, and B.H. Olson Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction Appl. Environ. Microbiol. 58 1992 2292 2295
27. M.J. Walker, C. Montemagno, J.C. Bryant, and W.C. Ghiorse Method detection limits of PCR and immunofluorescence assay for Cryptosporidium parvum in soil Appl. Environ. Microbiol. 64 1998 2281 2283
28. L.A. Ward, and Y. Wang Rapid methods to isolate Cryptosporidium DNA from frozen feces for PCR Diagn. Microbiol. Infect. Dis. 41 2001 37 42
29. I.G. Wilson Inhibition and facilitation of nucleic acid amplification Appl. Environ. Microbiol. 63 1997 3741 3751
30. L. Xiao, L. Escalante, C. Yang, I. Sulaiman, A.A. Escalante, R.J. Montali, R. Fayer, and A.A. Lal Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus Appl. Environ. Microbiol. 65 1999 1578 1583
31. G.P. Yakub, and K.L. Stadterman-Knauer Evaluation of immunomagnetic separation for recovery of Cryptosporidium parvum and Giardia duodenalis from high-iron matrices Appl. Environ. Microbiol. 66 2000 3628 3631