A method for rapid genetic integration into Plasmodium falciparum utilizing mycobacteriophage Bxb1 integrase

Methods in Molecular Biology

Volumn 634, Issue , 2010, Pages 87-100

  Adjalley, Sophie H ^a   Lee, Marcus C S ^a   Fidock, David A ^a  

^a NONE

Author keywords

Attb and attP sites; Bxb1 integrase; Integrative recombination; Malaria; Plasmodium falciparum; Transfection; Transgene expression

Indexed keywords

MYCOBACTERIUM PHAGE BXB1; PLASMODIUM FALCIPARUM;

INTEGRASE; PRIMER DNA;

ANIMAL; ARTICLE; ENZYMOLOGY; GENE; GENETIC RECOMBINATION; GENETICS; METABOLISM; MYCOBACTERIOPHAGE; NUCLEOTIDE SEQUENCE; PLASMID; PLASMODIUM FALCIPARUM;

ANIMALS; BASE SEQUENCE; DNA PRIMERS; GENES, PROTOZOAN; INTEGRASES; MYCOBACTERIOPHAGES; PLASMIDS; PLASMODIUM FALCIPARUM; RECOMBINATION, GENETIC;

EID: 79960300399     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-652-8_6     Document Type: Article

References (18)

1. Snow RW, Guerra CA, Noor AM, Myint HY, Hay SI (2005) The global distribution of clinical episodes of Plasmodium falciparum malaria. Nature 434:214-217
2. Crabb BS, Rug M, Gilberger TW, Thompson JK, Triglia T, Maier AG et al (2004) Transfection of the human malaria parasite Plasmodium falciparum. Methods Mol Biol 270:263-276
3. Groth AC, Calos MP (2004) Phage integrases: biology and applications. J Mol Biol 335:667-678
4. Ghosh P, Wasil LR, Hatfull GF (2006) Control of phage Bxb1 excision by a novel recombination directionality factor. PLoS Biol 4:e186
5. Thorpe HM, Wilson SE, Smith MC (2000) Control of directionality in the site-specific recombination system of the Streptomyces phage phiC31. Mol Microbiol 38:232-241
6. Thomason LC, Calendar R, Ow DW (2001) Gene insertion and replacement in Schizosaccharomyces pombe mediated by the Streptomyces bacteriophage phiC31 sitespecific recombination system. Mol Genet Genomics 265:1031-1038
7. Ghosh P, Kim AI, Hatfull GF (2003) The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB. Mol Cell 12:1101-1111
8. Nkrumah LJ, Muhle RA, Moura PA, Ghosh P, Hatfull GF, Jacobs WR Jr et al (2006) Efficient site-specific integration in Plasmodium falciparum chromosomes mediated by mycobacteriophage Bxb1 integrase. Nat Methods 3:615-621
9. Fidock DA, Wellems TE (1997) Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil. Proc Natl Acad Sci USA 94:10931-10936
10. Fidock DA, Nomura T, Wellems TE (1998) Cycloguanil and its parent compound proguanil demonstrate distinct activities against Plasmodium falciparum malaria parasites transformed with human dihydrofolate reductase. Mol Pharmacol 54:1140-1147
11. Su XZ, Wellems TE (1997) Plasmodium falciparum: a rapid DNA fingerprinting method using microsatellite sequences within var clusters. Exp Parasitol 86:235-236
12. Anderson TJ, Haubold B, Williams JT, Estrada-Franco JG, Richardson L, Mollinedo R et al (2000) Microsatellite markers reveal a spectrum of population structures in the malaria parasite Plasmodium falciparum. Mol Biol Evol 17:1467-1482
13. Trager W, Jensen JB (1976) Human malaria parasites in continuous culture. Science 193:673-675
14. Mamoun CB, Gluzman IY, Goyard S, Beverley SM, Goldberg DE (1999) A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum. Proc Natl Acad Sci USA 96:8716-8720
15. Wang P, Wang Q, Sims PF, Hyde JE (2002) Rapid positive selection of stable integrants following transfection of Plasmodium falciparum. Mol Biochem Parasitol 123:1-10
16. Makler MT, Hinrichs DJ (1993) Measurement of the lactate dehydrogenase activity of Plasmodium falciparum as an assessment of parasitemia. Am J Trop Med Hyg 48:205-210
17. Goodyer ID, Taraschi TF (1997) Plasmodium falciparum: a simple, rapid method for detecting parasite clones in microtiter plates. Exp Parasitol 86:158-160
18. Staalsoe T, Giha HA, Dodoo D, Theander TG, Hviid L (1999) Detection of antibodies to variant antigens on Plasmodium falciparum- infected erythrocytes by flow cytometry. Cytometry 35:329-336