Deep sequencing of the human TCRγ and TCRβ repertoires suggests that TCRβ rearranges after αβ and γδ T cell commitment

Science Translational Medicine

Volumn 3, Issue 90, 2011, Pages

  Sherwood, Anna M ^a   Desmarais, Cindy ^a   Livingston, Robert J ^a   Andriesen, Jessica ^a   Haussler, Maximilian ^b   Carlson, Christopher S ^c   Robins, Harlan ^c  

^a Adaptive TCR   (United States)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c Fred Hutchinson Cancer Research Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

T LYMPHOCYTE RECEPTOR BETA CHAIN; T LYMPHOCYTE RECEPTOR GAMMA CHAIN; LYMPHOCYTE ANTIGEN RECEPTOR;

ADULT; ALPHA BETA T LYMPHOCYTE; ARTICLE; CELL LINEAGE; CELL SELECTION; CONTROLLED STUDY; FEMALE; GAMMA DELTA T LYMPHOCYTE; GENE LOCUS; GENE REARRANGEMENT; HIGH THROUGHPUT SEQUENCING; HUMAN; HUMAN CELL; LYMPHOCYTE DIFFERENTIATION; MALE; NORMAL HUMAN; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL; SEQUENCE ALIGNMENT; T LYMPHOCYTE; T LYMPHOCYTE SUBPOPULATION; GENE DOSAGE; GENETICS; IMMUNOLOGY; METHODOLOGY; PHYSIOLOGY;

CELL LINEAGE; FEMALE; GENE DOSAGE; GENE REARRANGEMENT, BETA-CHAIN T-CELL ANTIGEN RECEPTOR; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MALE; RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA; RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA; T-LYMPHOCYTE SUBSETS; T-LYMPHOCYTES;

EID: 79960100655     PISSN: 19466234     EISSN: 19466242     Source Type: Journal    
DOI: 10.1126/scitranslmed.3002536     Document Type: Article

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46. Acknowledgments: H.R. thanks Y.-H. Chien for useful comments and suggestions. Funding: This study was supported by a grant from the NIH (AI081860). Author contributions: A.M.S., C.S.C., and H.R. designed the study. J.A. recruited subjects, acquired written informed consent, and arranged collection of blood samples. R.J.L. and A.M.S. sorted T cells, extracted DNA, and sequenced TCR and TCRβ chains. M.H. applied a computational tool of his own design to search the literature for TCR sequences matching those observed in this study. A.M.S., C.D., and H.R. analyzed the results, created the figures, and wrote the manuscript. All authors discussed the results and commented on the paper. Competing interests: A.M.S., C.D., R.J.L., and J.A. are employed by Adaptive TCR Corp., which commercialized, for research purposes, the TCRβ and TCRγ sequencing assays used in this manuscript under the brand name ImmunoSeq. C.S.C. and H.R. are co-founders of Adaptive TCR Corp. and own stock. H.R. and C.S.C. are inventors on the filed patent no. 003.001P2 titled "A method to measure immune diversity." The other authors declare they have no competing interests.