A genetic screen identifies BRCA2 and PALB2 as key regulators of G2 checkpoint maintenance

EMBO Reports

Volumn 12, Issue 7, 2011, Pages 705-712

  Menzel, Tobias ^a   Näsignhse Kumpf, Viola ^b   Kousholt, Arne Nedergaard ^a   Klein, Ditte Kjãorsgaard ^a   Lund Andersen, Christin ^b   Lees, Michael ^a   Johansen, Jens Vilstrup ^a   Syljuåsen, Randi G ^b   Sørensen, Claus Storgaard ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

AURORA A BORA PLK1; BRCA2 PALB2; DNA damage; G2 checkpoint; siRNA screens

Indexed keywords

AURORA A KINASE; BRCA2 PROTEIN; DNA; POLO LIKE KINASE 1; PROTEIN PLAB2; SMALL INTERFERING RNA; TUMOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL CYCLE G2 PHASE; CONTROLLED STUDY; DNA REPAIR; GENETIC SCREENING; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IONIZING RADIATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEPLETION; PROTEIN PROTEIN INTERACTION; RNA INTERFERENCE;

BRCA2 PROTEIN; CELL LINE; DNA DAMAGE; G2 PHASE; GENE LIBRARY; HCT116 CELLS; HELA CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; NUCLEAR PROTEINS; RECOMBINATION, GENETIC; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION; TUMOR SUPPRESSOR PROTEINS;

EID: 79959983476     PISSN: 1469221X     EISSN: 14693178     Source Type: Journal    
DOI: 10.1038/embor.2011.99     Document Type: Article

References (25)

1. Ayoub N, Rajendra E, Su X, Jeyasekharan AD, Mahen R, Venkitaraman AR (2009) The carboxyl terminus of Brca2 links the disassembly of Rad51 complexes to mitotic entry. Curr Biol 19: 1075-1085
2. Bassermann F, Frescas D, Guardavaccaro D, Busino L, Peschiaroli A, Pagano M (2008) The Cdc14B-Cdh1-Plk1 axis controls the G2 DNA-damage-response checkpoint. Cell 134: 256-267 (Pubitemid 352010329)
3. Birmingham A et al (2009) Statistical methods for analysis of high-throughput RNA interference screens. Nat Methods 6: 569-575
4. Bonner WM, Redon CE, Dickey JS, Nakamura AJ, Sedelnikova OA, Solier S, Pommier Y (2008) gH2AX and cancer. Nat Rev Cancer 8: 957-967
5. Boutros R, Dozier C, Ducommun B (2006) The when and wheres of CDC25 phosphatases. Curr Opin Cell Biol 18: 185-191 (Pubitemid 43375887)
6. Buisson R, Dion-Cote AM, Coulombe Y, Launay H, Cai H, Stasiak AZ, Stasiak A, Xia B, Masson JY (2010) Cooperation of breast cancer proteins PALB2 and piccolo BRCA2 in stimulating homologous recombination. Nat Struct Mol Biol 17: 1247-1254
7. Ciccia A, Elledge SJ (2010) The DNA damage response: making it safe to play with knives. Mol Cell 40: 179-204
8. Deckbar D, Birraux J, Krempler A, Tchouandong L, Beucher A, Walker S, Stiff T, Jeggo P, Lobrich M (2007) Chromosome breakage after G2 checkpoint release. J Cell Biol 176: 749-755 (Pubitemid 46425534)
9. Dray E et al (2010) Enhancement of RAD51 recombinase activity by the tumor suppressor PALB2. Nat Struct Mol Biol 17: 1255-1259
10. Hendzel MJ, Wei Y, Mancini MA, Van Hooser A, Ranalli T, Brinkley BR, Bazett-Jones DP, Allis CD (1997) Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation. Chromosoma 106: 348-360 (Pubitemid 27510848)
11. Hu B, Wang H, Wang X, Lu HR, Huang C, Powell SN, Huebner K, Wang Y (2005) Fhit and CHK1 have opposing effects on homologous recombination repair. Cancer Res 65: 8613-8616 (Pubitemid 41377345)
12. Iliakis G, Wang Y, Guan J, Wang H (2003) DNA damage checkpoint control in cells exposed to ionizing radiation. Oncogene 22: 5834-5847 (Pubitemid 37176704)
13. Jrgensen S, Elvers I, Trelle MB, Menzel T, Eskildsen M, Jensen ON, Helleday T, Helin K, Srensen CS (2007) The histone methyltransferase SET8 is required for S-phase progression. J Cell Biol 179: 1337-1345
14. König R et al (2007) A probability-based approach for the analysis of large-scale RNAi screens. Nat Methods 4: 847-849 (Pubitemid 350055583)
15. Lin HR, Ting NS, Qin J, Lee WH (2003) M phase-specific phosphorylation of BRCA2 by Polo-like kinase 1 correlates with the dissociation of the BRCA2-P/CAF complex. J Biol Chem 278: 35979-35987 (Pubitemid 37139916)
16. Macurek L, Lindqvist A, Lim D, Lampson MA, Klompmaker R, Freire R, Clouin C, Taylor SS, Yaffe MB, Medema RH (2008) Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Nature 455: 119-123
17. Seki A, Coppinger JA, Jang CY, Yates JR, Fang G (2008) Bora and the kinase Aurora a cooperatively activate the kinase Plk1 and control mitotic entry. Science 320: 1655-1658 (Pubitemid 351931258)
18. Syljua°sen RG, Jensen S, Bartek J, Lukas J (2006) Adaptation to the ionizing radiation-induced G2 checkpoint occurs in human cells and depends on checkpoint kinase 1 and Polo-like kinase 1 kinases. Cancer Res 66: 10253-10257 (Pubitemid 44799740)
19. Srensen CS, Hansen LT, Dziegielewski J, Syljua°sen RG, Lundin C, Bartek J, Helleday T (2005) The cell-cycle checkpoint kinase Chk1 is required for mammalian homologous recombination repair. Nat Cell Biol 7: 195-201
20. Srensen CS, Melixetian M, Klein DK, Helin K (2010) NEK11: linking CHK1 and CDC25A in DNA damage checkpoint signaling. Cell Cycle 9: 450-455
21. Tsvetkov L, Stern DF (2005) Phosphorylation of Plk1 at S137 and T210 is inhibited in response to DNA damage. Cell Cycle 4: 166-171 (Pubitemid 41038946)
22. Van Vugt MA, Bras A, Medema RH (2004) Polo-like kinase-1 controls recovery from a G2 DNA damage-induced arrest in mammalian cells. Mol Cell 15: 799-811 (Pubitemid 39194909)
23. Van Vugt MA, Bras A, Medema RH (2005) Restarting the cell cycle when the checkpoint comes to a halt. Cancer Res 65: 7037-7040 (Pubitemid 41161228)
24. Venkitaraman AR (2009) Linking the cellular functions of BRCA genes to cancer pathogenesis and treatment. Annu Rev Pathol 4: 461-487
25. Walter AO, Seghezzi W, Korver W, Sheung J, Lees E (2000) The mitotic serine/threonine kinase Aurora2/AIK is regulated by phosphorylation and degradation. Oncogene 19: 4906-4916