Re-engineering the discrimination between the oxidized coenzymes NAD + and NADP+ in clostridial glutamate dehydrogenase and a thorough reappraisal of the coenzyme specificity of the wild-type enzyme

FEBS Journal

Volumn 278, Issue 14, 2011, Pages 2460-2468

  Capone, Marina ^a,b   Scanlon, David ^a,c   Griffin, Joanna ^a   Engel, Paul C ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b Kuros Biosurgery AG   (Switzerland)

^c HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

burst kinetics; coenzyme purity; coenzyme specificity; glutamate dehydrogenase; site directed mutagenesis

Indexed keywords

BACTERIAL ENZYME; GLUTAMATE DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ANALYTICAL ERROR; ARTICLE; CATALYSIS; CLOSTRIDIUM; COENZYME; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ENGINEERING; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; FLOW KINETICS; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; WILD TYPE;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; CLOSTRIDIUM SYMBIOSUM; DRUG CONTAMINATION; GLUTAMATE DEHYDROGENASE; HYDROGEN-ION CONCENTRATION; INDICATORS AND REAGENTS; KINETICS; MUTANT PROTEINS; NAD; NADP; OSMOLAR CONCENTRATION; OXIDATION-REDUCTION; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 79959923488     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2011.08172.x     Document Type: Article

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