Redesign of the coenzyme specificity in L-Lactate dehydrogenase from Bacillus stearothermophilus using site-directed mutagenesis and media engineering

Protein Engineering

Volumn 12, Issue 10, 1999, Pages 851-856

  Holmberg, Niklas ^a   Ryde, Ulf ^a   Bülow, Leif ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Coenzyme specificity; Lactate dehydrogenase; Methanol; NADPH; Site directed mutagenesis

Indexed keywords

ASPARTIC ACID; LACTATE DEHYDROGENASE; METHANOL; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; COENZYME; CONTROLLED STUDY; ENZYME BINDING; ENZYME SPECIFICITY; GEOBACILLUS STEAROTHERMOPHILUS; MOLECULAR DYNAMICS; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN DOMAIN; SIMULATION; SITE DIRECTED MUTAGENESIS;

BACILLUS STEAROTHERMOPHILUS; BACTERIA (MICROORGANISMS); GEOBACILLUS STEAROTHERMOPHILUS; POSIBACTERIA;

EID: 0032744506     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/12.10.851     Document Type: Article

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