A real-time multiplex SYBR green i polymerase chain reaction assay for rapid screening of salmonella serotypes prevalent in the European Union

Foodborne Pathogens and Disease

Volumn 8, Issue 7, 2011, Pages 769-780

  Rajtak, Ursula ^a   Leonard, Nola ^a   Bolton, Declan ^a   Fanning, Séamus ^b  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b ASHTOWN FOOD RESEARCH CENTRE   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

AMOXICILLIN; AMPICILLIN; BACTERIAL ANTIGEN; CHLORAMPHENICOL; FLORFENICOL; SPECTINOMYCIN; STREPTOMYCIN; SULFONAMIDE; TETRACYCLINE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; EUROPEAN UNION; MULTIDRUG RESISTANCE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PREVALENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA; SEROTYPE;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; DNA PRIMERS; DRUG RESISTANCE, MULTIPLE, BACTERIAL; EUROPEAN UNION; GENES, BACTERIAL; HUMANS; MEAT PRODUCTS; MOLECULAR TYPING; ORGANIC CHEMICALS; PLASMIDS; POLYMERASE CHAIN REACTION; PREVALENCE; SALMONELLA; SALMONELLA INFECTIONS; SALMONELLA TYPHIMURIUM; SEROTYPING; SPECIES SPECIFICITY; VIRULENCE FACTORS;

ANIMALIA; SALMONELLA;

EID: 79959894571     PISSN: 15353141     EISSN: 15567125     Source Type: Journal    
DOI: 10.1089/fpd.2010.0768     Document Type: Article

References (28)

1. Agron PG, Walker RL, Kinde H, Sawyer SJ, Hayes DC, Wollard J, and Andersen GL. Identification by subtractive hybridization of sequences specific for Salmonella enterica serovar Enteritidis. Appl Environ Microbiol 2001;67:4984-4991. (Pubitemid 33701260)
2. Anonymous. Analysis of the baseline survey on the prevalence of Salmonella in holdings with breeding pigs, in the EU, 2008, Part A: Salmonella prevalence estimates. EFSA J 2009;7:1-93.
3. Anonymous. Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Getting Started Guide. Foster City, CA: Applied Biosystems, 2005.
4. Anonymous. Microbiology of Food and Animal Feeding Stuffs-Horizontal Method for the Detection of Salmonella (EN ISO 6579:2002). Geneva, Switzerland: International Organization for Standardization, 2002.
5. Anonymous. The community summary report on trends and sources of zoonoses, zoonotic agents, antimicrobial resistance and foodborne outbreaks in the European Union in 2006. EFSA J 2007;130:1-352.
6. Anonymous. The community summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in the European Union in 2008. EFSA J 2010;8:1-1496.
7. Chiu CH, Lin TY, and Ou JT. Prevalence of the virulence plasmids of nontyphoid Salmonella in the serovars isolated from humans and their association with bacteremia. Microbiol Immunol 1999;43:899-903. (Pubitemid 29459573)
8. Chiu CH, Su LH, Chu CH, Wang MH, Yeh CM, Weill FX, and Chu C. Detection of multidrug-resistant Salmonella enterica serovar Typhimurium phage types DT102, DT104, and U302 by multiplex PCR. J Clin Microbiol 2006;44:2354-2358. (Pubitemid 44092398)
9. Echeita M, Herrera S, Garaizar J, and Usera M. Multiplex PCRbased detection and identification of themost common Salmonella second-phase flagellar antigens. Res Microbiol 2002;153:107-113. (Pubitemid 34164539)
10. Echeita M, Herrera S, and Usera M. Atypical, fljB-negative Salmonella enterica subsp. enterica strain of serovar 4,5,12:i:- appears to be a monophasic variant of serovar Typhimurium. J Clin Microbiol 2001;39:2981-2983. (Pubitemid 32744026)
11. Heid CA, Stevens J, Livak KJ, and Williams PM. Real time quantitative PCR. Genome Res 1996;6:986-994. (Pubitemid 26388091)
12. Hendriksen RS, Mikoleit M, Carlson VP, Karlsmose S, Vieira AR, Jensen AB, Seyfarth AM, DeLong SM, Weill FX, Lo Fo Wong DMA, Angulo FJ, Wegener HC, and Aarestrup FM. WHO Global Salm-Surv External Quality Assurance System (EQAS) for serotyping of Salmonella isolates, 2000-2007. J Clin Microbiol 2009;47:2729-2736.
13. Herrera-León S, Ramiro R, Arroyo M, Díez R, Usera MA, and Echeita MA. Blind comparison of traditional serotyping with three multiplex PCRs for the identification of Salmonella serotypes. Res Microbiol 2007;158:122-127. (Pubitemid 46341716)
14. Hong Y, Liu T, LeeMD,Hofacre CL, MaierM, White DG, Ayers S, Wang L, Berghaus R, and Maurer JJ. Rapid screening of Salmonella enterica serovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting theOandHantigen alleles.BMC Microbiol 2008;8:178.
15. Kim DW. Real time quantitative PCR. Exp Mol Med 2001;33: 101-109.
16. Kim S, Frye JG, Hu J, Fedorka-Cray PJ, Gautom R, and Boyle DS. Multiplex PCR-based method for identification of common clinical serotypes of Salmonella enterica subsp. enterica. J Clin Microbiol 2006;44:3608-3615. (Pubitemid 46768803)
17. Lim BK and Thong KL. Application of PCR-based serogrouping of selected Salmonella serotypes in Malaysia. J Infect Dev Ctries 2009;3:420-428.
18. Macnab RM. Genetics and biogenesis of bacterial flagella. Annu Rev Genet 1992;26:131-158. (Pubitemid 23022606)
19. McQuiston JR, Parrenas R, Ortiz-Rivera M, Gheesling L, Brenner F, and Fields PI. Sequencing and comparative analysis of flagellin genes fliC, fljB, and flpA from Salmonella. J Clin Microbiol 2004;42:1923-1932. (Pubitemid 38637803)
20. Mulvey MR, Boyd DA, Olson AB, Doublet B, and Cloeckaert A. The genetics of Salmonella genomic island 1. Microbes Infect 2006;8:1915-1922. (Pubitemid 44144557)
21. Olsen EV, Gibbins CS, and Grayson JK. Real-time FRET PCR assay for Salmonella enterica serotype detection in food. Mil Med 2009;174:983-990.
22. O'Regan E, Catherine B, Sheila MG, Thomas B, Geraldine D, Paul W, and Séamus F. Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples. BMC Microbiol 2008;8:156.
23. Perugini AG, Carullo MR, Esposito A, Caligiuri V, Capuano F, Galiero G, and Iovane G. Characterization of antimicrobial resistant Salmonella enterica serovars Enteritidis and Typhimurium isolates from animal and food in Southern Italy. Vet Res Commun 2010;34:387-392.
24. Ririe KM, Rasmussen RP, and Wittwer CT. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem 1997;245:154-160. (Pubitemid 27082244)
25. Rychlík I, Gregorová D, and Hradecká H. Distribution and function of plasmids in Salmonella enterica. Vet Microbiol 2006; 112:1-10. (Pubitemid 41814709)
26. Targant H, Ponsin C, Brunet C, Doublet B, Cloeckaert A, Madec JY, and Meunier D. Characterization of resistance genes in multidrug-resistant Salmonella enterica serotype Typhimurium isolated from diseased cattle in France (2002 to 2007). Foodborne Pathog Dis 2010;7:419-425.
27. Woods DF, Reen FJ, Gilroy D, Buckley J, Frye JG, and Boyd EF. Rapid multiplex PCR and real time TaqMan PCR assays for the detection of Salmonella and the highly virulent serovars Choleraesuis and Paratyphi C. J Clin Microbiol 2008;46:4018-4022.
28. Yoshitomi KJ, Jinneman KC, and Weagant SD. Detection of Shiga toxin genes stx1, stx2, and the +93 uidA mutation of E. coli O157: H7/H-using SYBR-Green I in a real-time multiplex PCR. Mol Cell Probes 2006;20:31-41. (Pubitemid 43254874)