Knockdown of α-synuclein enhances susceptibility to staurosporine-induced apoptosis in human melanoma SK-MEL28 cells

Journal of Biological Sciences

Volumn 11, Issue 2, 2011, Pages 135-145

  Choong, Chi Jing ^a   Say, Yee How ^a  

^a UNIVERSITI TUNKU ABDUL RAHMAN   (Malaysia)

Author keywords

Synuclein; Apoptosis; Melanoma; shRNA knockdown; Staurosporine

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ALPHA SYNUCLEIN; CASPASE 9; CELL MARKER; PROTEIN BAX; PROTEIN BCL 2; PROTEIN BCL X; PUROMYCIN; SHORT HAIRPIN RNA; STAUROSPORINE;

APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CELL CYCLE; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MELANOMA CELL; MORPHOLOGY; NUCLEOTIDE SEQUENCE; PROTEIN BLOOD LEVEL; PROTEIN DEPLETION; PROTEIN EXPRESSION; PROTEIN TARGETING; WESTERN BLOTTING;

EID: 79959859370     PISSN: 17273048     EISSN: 18125719     Source Type: Journal    
DOI: 10.3923/jbs.2011.135.145     Document Type: Article

References (37)

1. Ahmad, M., S. Attoub, M.N. Singh, F.L. Martin and O.M.A. El-Agnaf, 2007. γ-Synuclein and the progression of cancer. FASEB J., 21: 3419-3430.
2. Bruening, W., B.I. Giasson, A.J. Klein-Szanto, V.M. Lee, J.Q. Trojanowski and A.K. Godwin, 2000. Synucleins are expressed in the majority of breast and ovarian carcinomas and in preneoplastic lesions of the ovary. Cancer, 88: 2154-2163.
3. Chang, J., Y. Hsu, P. Kuo, Y. Kuo, L. Chiang and C. Lin, 2005. Increase of Bax/ Bcl-XL ratio and arrest of cell cycle by luteolin in immortalized human hepatoma cell line. Life Sci., 76: 1883-1893.
4. Czekierdowski, A. and S. Czekierdowska, 2007. SNCG (synuclein, gamma (breast cancer-specific protein 1). Atlas Genet Cytogenet Oncol Haematol. June 2007. http://AtlasGeneticsOncology.org/Genes/SNCGID42343chl0q23.html.
5. Da Costa, A.C., E. Paitel, B. Vincent and F. Checler, 2002. Alpha-synuclein lowers p53-dependent apoptotic response of neuronal cells. Abolishment by 6-hydroxydopamine and implication for Parkinson's disease. J. Biol. Chem., 277: 50980-50984.
6. Da Costa, C.A., K. Ancolio and F. Checler, 2000. Wild-type but not parkinson's disease-related Ala-53→Thr mutant a-Synuclein protects neuronal cells from apoptotic stimuli. J. Biol. Chem., 275: 24065-24069.
7. Duan, H., K. Orth, A.M. Chinnaiyan, G.G. Poirier, C.J. Froelich, W.W. He and V.M. Dixit, 1996. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J. Biol. Chem., 271: 16720-16724.
8. El-Agnaf, O.M. and G.B. Irvine, 2000. Review: Formation and properties of amyloid-like fibrils derived from α-Synuclein and related proteins. J. Struct. Biol., 130: 300-309.
9. George, J.M., H. Jin, W.S. Woods and D.F. Clayton, 1995. Characterization of a novel protein regulated during the critical period for song learning in the zebra finch. Neuron, 15: 361-372.
10. + increases a-Synuclein expression and ERK/MAP-kinase phosphorylation in human neuroblastoma SH-SY5Y cells. Brain Res., 935: 32-39.
11. Hashimoto, M., L.J. Hsu, E. Rockenstein, T. Takenouchi, M. Mallory and E. Masliah, 2002. α-Synuclein protects against oxidative stress via inactivation of the c-Jun N-terminal kinase stress-signaling pathway in neuronal cells. J. Biol. Chem., 277: 11465-11472.
12. Hua, H., L. Xu, J. Wang, J. Jing, T. Luo and Y. Jiang, 2009. Up-regulation of gamma-synuclein contributes to cancer cell survival under endoplasmic reticulum stress. J. Pathol, 217: 507-515.
13. Lavedan, C, 1998. The synuclein family. Genome Res., 8: 871-880.
14. Lee, S.S., Y.M. Kim, E. Junn, G. Lee and K.H. Park, et el., 2003. Cell cycle aberrations by alpha-synuclein over-expression and cyclin B immunoreactivity in Lewy bodies. Neurobiol. Aging, 24: 687-696.
15. Li, P., D. Nijhawan, I. Budihardjo, S.M. Srinivasula, M. Ahmad, E.S Alnemn and X. Wang, 1997. Cytochrome c and dATP-dependent formation of Apaf-l/caspase-9 complex initiates an apoptotic protease cascade. Cell, 91: 479-489.
16. Li, X., M. Marani, R. Mannucci, B. Kinsey and F. Andriani, et al, 2001. Overexpressionof BCL-X(L) underlies the molecular basis for resistance to staurosporine-induced apoptosis in PC-3 cells. Cancer Res.,61: 1699-1706.
17. Liu, H., Y. Zhou, S.E. Boggs, S.A. Belinsky and J. Liu, 2007. Cigarette smoke induces demethylation of prometastatic oncogene synuclein-gamma in lung cancer cells by downregulation of DNMT3B. Oncogene, 26: 5900-5910.
18. Maries, E., B. Dass, T.J. Collier, J.H. Kordower and K. Steece-Collier, 2003. The role of α-Synuclein in Parkinson's disease: Insights from animal models. Nat. Rev. Neurosci., 4: 727-738.
19. Matsuo, Y. and T. Kamitani, 2010. Parkinson's disease-related protein, α-Synuclein, in malignant melanoma. PLoS ONE, 5: el 0481-el 0481.
20. Moffat, J., D.A. Grueneberg, X. Yang, S.Y. Kim and A.M. Kloepfer et al, 2006. A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Cell, 124: 1283-1298.
21. Morgan, J., A.V. Hoekstra, E. Chapman-Davis, J.L. Hardt, J.J. Kim and B.M. Buttin, 2009. Synuclein-γ (SNCG) may be a novel prognostic biomarker in uterine papillary serous carcinoma. Gynecol. Oncol., 114: 293-298.
22. Oltvai, Z.N. and S.J. Korsmeyer, 1994. Checkpoints of dueling dimers foil death wishes. Cell, 79: 189-192.
23. Ostrerova, N., L. Petrucelli, M. Fairer, N. Mehta, P. Choi, J. Hardy and B. Wolozin, 1999. α-Synuclein shares physical and functional homology with 14-3-3 proteins. J. Neurosci., 19: 5782-5791.
24. Pan, Z.Z., W. Bruening, B.I. Giasson, V.M. Lee and A.K. Godwin, 2002. γ-synuclein promotes cancer cell survival and inhibits stress-and chemotherapy drug-induced apoptosis by modulating MAPK pathways. J. Biol. Chem., 277: 35050-35060.
25. Perrin, R. J., W.S. Woods, D.F. Clayton and J.M. George, 2000. Interaction of human α-Synuclein and parkinsons disease variants with phospholipids: Structural Analysis Using Site-Directed Mutagenesis. J. Biol. Chem., 275: 34393-34398.
26. Raisova, M., A.M. Hossini, J. Eberle, C. Riebeling, T. Wieder et al, 2001. The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis. J. Invest. Dermatol., 117: 333-340.
27. Sapru, M.K., J.W. Yates, S. Hogan, L. Jiang, J. Halter and M.C. Bohn, 2006. Silencing of human α-Synuclein in vitro and in rat brain using lentiviral-mediated RNAi. Exp. Neurol., 198: 382-390.
28. Seo, J.H., J.C. Rah, S.H. Choi, J.K. Shin and K. Min, et al, 2002. α-Synuclein regulates neuronal survival via Bcl-2 family expression and PI3/Akt kinase pathway. FASEB J., 16: 1826-1828.
29. Sidhu, A., C. Wersinger, C.E. Moussa and P. Vernier, 2004. The role of α-Synuclein in both neuroprotection and neurodegeneration. Ann. NY. Acad. Sci., 1035: 250-270.
30. Souza, J.M., B.I. Giasson, V.M. Lee and H. Ischiropoulos, 2000. Chaperone-like activity of synucleins. FEBS Lett, 474: 116-119.
31. + induced mitochondrial dysfunction of SH-SY5Y cells. Brain Res., 1292: 173-179.
32. Yanagawa, N., G. Tamura, T. Honda, M. Endoh, S. Nishizuka and T. Motoyama, 2004. Demethylation of the synuclein γ gene CpG island in primary gastric cancers and gastric cancer cell lines. Clin. Cancer Res., 10: 2447-2451.
33. Ye, Q., M.H. Zheng, Q. Cai, B. Feng and X.H. Chen et al., 2008. Aberrant expression and demethylation of γ-synuclein in colorectal cancer, correlated with progression of the disease. Cancer Sci., 99: 1924-1932.
34. Yuan, B., R. Latek, M. Hossbach, T. Tuschl and F. Lewitter, 2004. siRNA Selection Server: An automated siRNA oligonucleotide prediction server. Nucleic Acids Res., 32: W130-W134.
35. Zhang, B.F., F.F. Peng, J.Z. Zhang and D.C. Wu, 2003. Staurosporine induces apoptosis inNG108-15 cells. Acta Pharmacol. Sin., 24: 663-669.
36. Zhang, X.D., S.K. Gillespie and P. Hersey, 2004. Staurosporine induces apoptosis of melanoma by both caspase-dependent and-independent apoptotic pathways. Mol. Cancer Ther., 3: 187-197.
37. Zhao, W., H. Liu, W. Liu, Y. Wu and W. Chen et al, 2006. Abnormal activation of the synuclein-γ gene in hepatocellular carcinomas by epigenetic alteration. Int. J. Oncol, 28: 1081-1088.