Panel Development for Multicolor Flow-Cytometry Testing of Proliferation and Immunophenotype in hMSCs

Methods in Molecular Biology

Volumn 698, Issue , 2011, Pages 367-385

  Bradford, Jolene A ^a   Clarke, Scott T ^b  

^a Life Technologies   (United States)

^b NONE

Author keywords

Click chemistry; EdU; Expansion; Flow cytometry; hMSC; Immunophenotype; ISCT; Mesenchymal stem cells; Multicolor; Proliferation

Indexed keywords

URIDINE;

ANIMAL; ARTICLE; CELL PROLIFERATION; CHEMISTRY; COLOR; CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECT; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; HUMAN; IMMUNOLOGY; IMMUNOPHENOTYPING; INSTRUMENTATION; MESENCHYMAL STEM CELL; METHODOLOGY;

ANIMALS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; COLOR; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; IMMUNOPHENOTYPING; MESENCHYMAL STEM CELLS; URIDINE;

EID: 79959783742     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-999-4_27     Document Type: Chapter

References (32)

1. Chamberlain, G., Fox, J., Ashton, B., and Middleton, J. (2007) Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing, Stem Cells 25, 2739–2749.
2. Dominici, M., Le Blanc, K., Mueller, I., Slaper-Cortenbach, I., Marini, F., Krause, D., Deans, R., Keating, A., Prockop, D., and Horwitz, E. (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement, Cytotherapy 8, 315–317.
3. Roche, S., Delorme, B., Oostendorp, R. A., Barbet, R., Caton, D., Noel, D., Boumediene, K., Papadaki, H. A., Cousin, B., Crozet, C., Milhavet, O., Casteilla, L., Hatzfeld, J., Jorgensen, C., Charbord, P., and Lehmann, S. (2009) Comparative proteomic analysis of human mesenchymal and embryonic stem cells: towards the definition of a mesenchymal stem cell proteomic signature, Proteomics 9, 223–232.
4. Lin, G., Garcia, M., Ning, H., Banie, L., Guo, Y. L., Lue, T. F., and Lin, C. S. (2008) Defining stem and progenitor cells within adipose tissue, Stem Cells Dev 17, 1053–1063.
5. Gimble, J. M., Katz, A. J., and Bunnell, B. A. (2007) Adipose-derived stem cells for regenerative medicine, Circ Res 100, 1249–1260.
6. Tung, J. W., Parks, D. R., Moore, W. A., and Herzenberg, L. A. (2004) Identification of B-cell subsets: an exposition of 11-color (Hi-D) FACS methods, Methods Mol Biol 271, 37–58.
7. Perfetto, S. P., Chattopadhyay, P. K., and Roederer, M. (2004) Seventeen-colour flow cytometry: unravelling the immune system, Nat Rev Immunol 4, 648–655.
8. Wood, B. (2006) 9-color and 10-color flow cytometry in the clinical laboratory, Arch Pathol Lab Med 130, 680–690.
9. Buck, S. B., Bradford, J., Gee, K. R., Agnew, B. J., Clarke, S. T., and Salic, A. (2008) Detection of S-phase cell cycle progression using 5-ethynyl-2¢-deoxyuridine incorpora- tion with click chemistry, an alternative to using 5-bromo-2¢-deoxyuridine antibodies, Biotechniques 44, 927–929.
10. Salic, A., and Mitchison, T. J. (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo, Proc Natl Acad Sci U S A 105, 2415–2420.
11. Meldal, M., and Tornoe, C. W. (2008) Cu-catalyzed azide-alkyne cycloaddition, Chem Rev 108, 2952–3015.
12. Tornoe, C. W., Christensen, C., and Meldal, M. (2002) Peptidotriazoles on solid phase: [1,2,3]-triazoles by regiospecific copper(i)-catalyzed 1,3-dipolar cycloadditions of terminal alkynes to azides, J Org Chem 67, 3057–3064.
13. Ormerod, M. G. (2008) Flow cytometry – a basic introduction, at http:flowbook.denovo-software.com
14. Baumgarth, N., and Roederer, M. (2000) A practical approach to multicolor flow cytometry for immunophenotyping, J Immunol Methods 243, 77–97.
15. Maecker, H. T., Frey, T., Nomura, L. E., and Trotter, J. (2004) Selecting fluorochrome conjugates for maximum sensitivity, Cytometry A 62, 169–173.
16. Maecker, H. T., and Trotter, J. (2008) Selecting reagents for multicolor flow cytometry, BD Biosciences Application Note.
17. Roederer, M. (1996) Conjugation of monoclonal antibodies, at http://www.drmr.com/abcon/index.html
18. Roederer, M., De Rosa, S., Gerstein, R., Anderson, M., Bigos, M., Stovel, R., Nozaki, T., Parks, D., and Herzenberg, L. (1997) 8 color, 10-parameter flow cytometry to elucidate complex leukocyte heterogeneity, Cytometry 29, 328–339.
19. Diermeier-Daucher, S., Clarke, S. T., Hill, D., Vollmann-Zwerenz, A., Bradford, J. A., and Brockhoff, G. (2009) Cell type specific applicability of 5-ethynyl-2¢-deoxyuridine (EdU) for dynamic proliferation assessment in flow cytometry, Cytometry A 75, 535–546.
20. Maecker, H. T., and Trotter, J. (2006) Flow cytometry controls, instrument setup, and the determination of positivity, Cytometry A 69, 1037–1042.
21. Bayer, J., Grunwald, D., Lambert, C., Mayol, J. F., and Maynadie, M. (2007) Thematic workshop on fluorescence compensation settings in multicolor flow cytometry, Cytometry B Clin Cytom 72, 8–13.
22. Herzenberg, L. A., Tung, J., Moore, W. A., and Parks, D. R. (2006) Interpreting flow cytometry data: a guide for the perplexed, Nat Immunol 7, 681–685.
23. McLaughlin, B. E., Baumgarth, N., Bigos, M., Roederer, M., De Rosa, S. C., Altman, J. D., Nixon, D. F., Ottinger, J., Oxford, C., Evans, T. G., and Asmuth, D. M. (2008) Nine-color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part I: Panel design by an empiric approach, Cytometry A 73, 400–410.
24. Roederer, M. (2001) Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats, Cytometry 45, 194–205.
25. Invitrogen. Spectra Viewer, at http://www. invitrogen.com/site/us/en/home/support/research-tools/fluorescence-SpectraViewer. html
26. Shankey, T. V., Rabinovitch, P. S., Bagwell, B., Bauer, K. D., Duque, R. E., Hedley, D. W., Mayall, B. H., Wheeless, L., and Cox, C. (1993) Guidelines for implementation of clinical DNA cytometry. International Society for Analytical Cytology, Cytometry 14, 472–477.
27. Wersto, R. P., Chrest, F. J., Leary, J. F., Morris, C., Stetler-Stevenson, M. A., and Gabrielson, E. (2001) Doublet discrimination in DNA cell-cycle analysis, Cytometry 46, 296–306.
28. Givan, A. L. (2001) Flow Cytometry: First Principles, Wiley, New York.
29. Bagwell, C. B. (2004) DNA histogram analysis for node-negative breast cancer, Cytometry A 58, 76–78.
30. Telford, W. G. (2004) Analysis of UV-excited fluorochromes by flow cytometry using near-ultraviolet laser diodes, Cytometry A 61, 9–17.
31. Shapiro, H. (2003) Practical Flow Cytometry, Fourth Edition, John, Hobboken.
32. ® nanocrystal primary antibody conjugates in flow cytometry, Invitrogen Application Note, at http://www. probes.i nvi trogen.com/products/qdot/images/O-073210%20QDot%20AppNote_ HRF.pdf