An optimized protocol for DNA extraction from wheat seeds and loop-mediated isothermal amplification (LAMP) to detect Fusarium graminearum contamination of wheat grain

International Journal of Molecular Sciences

Volumn 12, Issue 6, 2011, Pages 3459-3472

  Abd Elsalam, Kamel ^a,b,c   Bahkali, Ali ^a,b   Moslem, Mohamed ^a   Amin, Osama E ^a   Niessen, Ludwig ^d  

^a KING SAUD UNIVERSITY   (Saudi Arabia)

^b KING SAUD UNIVERSITY   (Saudi Arabia)

^c Agricultural Research Center   (Egypt)

^d TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

DNA isolation; LAMP; Loop mediated isothermal amplification; PCR; Seed borne fungi

Indexed keywords

GENOMIC DNA; RIBONUCLEASE; FUNGAL DNA; PLANT DNA; PRIMER DNA;

AGAR GEL ELECTROPHORESIS; ARTICLE; DNA EXTRACTION; DNA ISOLATION; DNA PURIFICATION; FUNGAL CONTAMINATION; FUNGUS CULTURE; FUNGUS ISOLATION; FUSARIUM GRAMINEARUM; GENETIC VARIABILITY; GERMINATION; INTER SIMPLE SEQUENCE REPEAT; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; NONHUMAN; PLANT SEED; RELIABILITY; WHEAT; FUSARIUM; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; NUCLEIC ACID AMPLIFICATION; POLYMERASE CHAIN REACTION;

FUNGI; FUSARIA; GIBBERELLA ZEAE; TRITICUM AESTIVUM;

DNA PRIMERS; DNA, FUNGAL; DNA, PLANT; FUSARIUM; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; RIBONUCLEASES; SEEDS; TRITICUM;

EID: 79959708968     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms12063459     Document Type: Article

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