RNAi-mediated inhibition of the desmosomal cadherin (desmoglein 3) impairs epithelial cell proliferation

Cell Proliferation

Volumn 44, Issue 4, 2011, Pages 301-310

  Mannan, T ^a   Jing, S ^b   Foroushania, S H ^a   Fortune, F ^a   Wan, H ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^b NORFOLK AND NORWICH UNIVERSITY HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BROXURIDINE; DESMOGLEIN 3; SMALL INTERFERING RNA;

ARTICLE; CELL ADHESION; CELL COUNT; CELL PROLIFERATION; COLONY FORMING CELL; CONFOCAL MICROSCOPY; CONTROLLED STUDY; EPITHELIUM CELL; FLUORESCENCE ACTIVATED CELL SORTING; FRAGMENTATION REACTION; GENE SILENCING; HANGING DROP VAPOR DIFFUSION METHOD; HUMAN; HUMAN CELL; INHIBITION KINETICS; NUCLEOTIDE SEQUENCE; RNA INTERFERENCE; WESTERN BLOTTING;

ANIMALS; CELL LINE; CELL PROLIFERATION; DESMOGLEIN 3; DESMOSOMES; DOGS; EPITHELIAL CELLS; HUMANS; RNA INTERFERENCE;

EID: 79959703249     PISSN: 09607722     EISSN: 13652184     Source Type: Journal    
DOI: 10.1111/j.1365-2184.2011.00765.x     Document Type: Article

References (41)

1. Bass-Zubek AE, Godsel LM, Delmar M, Green KJ (2009) Plakophilins: multifunctional scaffolds for adhesion and signaling. Curr. Opin. Cell Biol. 21, 708-716.
2. Garrod D, Kimura TE (2008) Hyper-adhesion: a new concept in cell-cell adhesion. Biochem. Soc. Trans. 36, 195-201.
3. Sharma P, Mao X, Payne AS (2007) Beyond steric hindrance: the role of adhesion signaling pathways in the pathogenesis of pemphigus. J. Dermatol. Sci. 48, 1-14.
4. Garrod D, Chidgey M (2008) Desmosome structure, composition and function. Biochim. Biophys. Acta 1778, 572-587.
5. Allen E, Yu QC, Fuchs E (1996) Mice expressing a mutant desmosomal cadherin exhibit abnormalities in desmosomes, proliferation, and epidermal differentiation. J. Cell Biol. 133, 1367-1382.
6. Merritt AJ, Berika MY, Zhai W, Kirk SE, Ji B, Hardman MJ et al. (2002) Suprabasal desmoglein 3 expression in the epidermis of transgenic mice results in hyperproliferation and abnormal differentiation. Mol. Cell. Biol. 22, 5846-5858.
7. Muller EJ, Williamson L, Kolly C, Suter MM (2008) Outside-in signaling through integrins and cadherins: a central mechanism to control epidermal growth and differentiation? J. Invest. Dermatol. 128, 501-516.
8. Williamson L, Raess NA, Caldelari R, Zakher A, de Bruin A, Posthaus H et al. (2006) Pemphigus vulgaris identifies plakoglobin as key suppressor of c-Myc in the skin. EMBO J. 25, 3298-3309.
9. Williamson L, Suter MM, Olivry T, Wyder M, Muller EJ (2007) Upregulation of c-Myc may contribute to the pathogenesis of canine pemphigus vulgaris. Vet. Dermatol. 18, 12-17.
10. Teh MT, Parkinson EK, Thurlow JK, Liu F, Fortune F, Wan H (2011) A molecular study of desmosomes identifies a desmoglein isoform switch in head and neck squamous cell carcinoma. J. Oral Pathol. Med. 40, 67-76.
11. Yashiro M, Nishioka N, Hirakawa K (2006) Decreased expression of the adhesion molecule desmoglein-2 is associated with diffuse-type gastric carcinoma. Eur. J. Cancer 42, 2397-2403.
12. Brennan D, Mahoney MG (2009) Increased expression of Dsg2 in malignant skin carcinomas: a tissue-microarray based study. Cell Adh. Migr. 3, 148-154.
13. Chen YJ, Chang JT, Lee L, Wang HM, Liao CT, Chiu CC et al. (2007) DSG3 is overexpressed in head neck cancer and is a potential molecular target for inhibition of oncogenesis. Oncogene 26, 467-476.
14. Savci-Heijink CD, Kosari F, Aubry MC, Caron BL, Sun Z, Yang P et al. (2009) The role of desmoglein-3 in the diagnosis of squamous cell carcinoma of the lung. Am. J. Pathol. 174, 1629-1637.
15. Armstrong DK, McKenna KE, Purkis PE, Green KJ, Eady RA, Leigh IM et al. (1999) Haploinsufficiency of desmoplakin causes a striate subtype of palmoplantar keratoderma [published erratum appears in Hum Mol Genet 1999 May;8(5):943]. Hum. Mol. Genet. 8, 143-148.
16. Barber AG, Wajid M, Columbo M, Lubetkin J, Christiano AM (2007) Striate palmoplantar keratoderma resulting from a frameshift mutation in the desmoglein 1 gene. J. Dermatol. Sci. 45, 161-166.
17. Cabral RM, Liu L, Hogan C, Dopping-Hepenstal PJ, Winik BC, Asial RA et al. (2010) Homozygous mutations in the 5' region of the JUP gene result in cutaneous disease but normal heart development in children. J. Invest. Dermatol. 130, 1543-1550.
18. Hunt DM, Rickman L, Whittock NV, Eady RA, Simrak D, Dopping-Hepenstal PJ et al. (2001) Spectrum of dominant mutations in the desmosomal cadherin desmoglein 1, causing the skin disease striate palmoplantar keratoderma. Eur. J. Hum. Genet. 9, 197-203.
19. McGrath JA, McMillan JR, Shemanko CS, Runswick SK, Leigh IM, Lane EB et al. (1997) Mutations in the plakophilin 1 gene result in ectodermal dysplasia/skin fragility syndrome. Nat. Genet. 17, 240-244.
20. Wan H, South AP, Hart IR (2007) Increased keratinocyte proliferation initiated through downregulation of desmoplakin by RNA interference. Exp. Cell Res. 313, 2336-2344.
21. Wan H, Stone MG, Simpson C, Reynolds LE, Marshall JF, Hart IR et al. (2003) Desmosomal proteins, including desmoglein 3, serve as novel negative markers for epidermal stem cell-containing population of keratinocytes. J. Cell Sci. 116, 4239-4248.
22. Wan H, Yuan M, Simpson C, Allen K, Gavins FN, Ikram MS et al. (2007) Stem/progenitor cell-like properties of desmoglein 3dim cells in primary and immortalized keratinocyte lines. Stem Cells 25, 1286-1297.
23. Boukamp P, Petrussevska RT, Breitkreutz D, Hornung J, Markham A, Fusenig NE (1988) Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J. Cell Biol. 106, 761-771.
24. Denning MF, Guy SG, Ellerbroek SM, Norvell SM, Kowalczyk AP, Green KJ (1998) The expression of desmoglein isoforms in cultured human keratinocytes is regulated by calcium, serum, and protein kinase C. Exp. Cell Res. 239, 50-59.
25. Karst W, Merker HJ (1988) The differentiation behaviour of MDCK cells grown on matrix components and in collagen gels. Cell Differ. 22, 211-224.
26. Schoop VM, Mirancea N, Fusenig NE (1999) Epidermal organization and differentiation of HaCaT keratinocytes in organotypic coculture with human dermal fibroblasts. J. Invest. Dermatol. 112, 343-353.
27. Yamada S, Nelson WJ (2007) Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell-cell adhesion. J. Cell Biol. 178, 517-527.
28. Wan H, Dopping-Hepenstal PJ, Gratian MJ, Stone MG, McGrath JA, Eady RA (2003) Desmosomes exhibit site-specific features in human palm skin. Exp. Dermatol. 12, 378-388.
29. Aijaz S, Sanchez-Heras E, Balda MS, Matter K (2007) Regulation of tight junction assembly and epithelial morphogenesis by the heat shock protein Apg-2. BMC. Cell Biol. 8, 49.
30. Tsang SM, Liu L, Teh MT, Wheeler A, Grose R, Hart IR et al. (2010) Desmoglein 3, via an interaction with E-cadherin, is associated with activation of Src. PLoS One 5, e14211.
31. Kimura TE, Merritt AJ, Garrod DR (2007) Calcium-independent desmosomes of keratinocytes are hyper-adhesive. J. Invest. Dermatol. 127, 775-781.
32. Getsios S, Amargo EV, Dusek RL, Ishii K, Sheu L, Godsel LM et al. (2004) Coordinated expression of desmoglein 1 and desmocollin 1 regulates intercellular adhesion. Differentiation 72, 419-433.
33. Getsios S, Huen AC, Green KJ (2004) Working out the strength and flexibility of desmosomes. Nat. Rev. Mol. Cell Biol. 5, 271-281.
34. Jamora C, Fuchs E (2002) Intercellular adhesion, signalling and the cytoskeleton. Nat. Cell Biol. 4, E101-E108.
35. Yin T, Green KJ (2004) Regulation of desmosome assembly and adhesion. Semin. Cell Dev. Biol. 15, 665-677.
36. Garrod DR, Merritt AJ, Nie Z (2002) Desmosomal adhesion: structural basis, molecular mechanism and regulation (Review). Mol. Membr. Biol. 19, 81-94.
37. Garrod DR, Merritt AJ, Nie Z (2002) Desmosomal cadherins. Curr. Opin. Cell Biol. 14, 537-545.
38. Brennan D, Hu Y, Joubeh S, Choi YW, Whitaker-Menezes D, O'Brien T et al. (2007) Suprabasal Dsg2 expression in transgenic mouse skin confers a hyperproliferative and apoptosis-resistant phenotype to keratinocytes. J. Cell Sci. 120, 758-771.
39. Hardman MJ, Liu K, Avilion AA, Merritt A, Brennan K, Garrod DR et al. (2005) Desmosomal cadherin misexpression alters {beta}-catenin stability and epidermal differentiation. Mol. Cell. Biol. 25, 969-978.
40. Runswick SK, O'Hare MJ, Jones L, Streuli CH, Garrod DR (2001) Desmosomal adhesion regulates epithelial morphogenesis and cell positioning. Nat. Cell Biol. 3, 823-830.
41. Getsios S, Simpson CL, Kojima S, Harmon R, Sheu LJ, Dusek RL et al. (2009) Desmoglein 1-dependent suppression of EGFR signaling promotes epidermal differentiation and morphogenesis. J. Cell Biol. 185, 1243-1258.