Laser scanning cytometry of mitosis: State and stage analysis

Methods in Cell Biology

Volumn 102, Issue , 2011, Pages 341-372

  Stefan, Tammy ^a   Jacobberger, James W ^a  

^a CASE WESTERN RESERVE UNIVERSITY   (United States)

Author keywords

Cell cycle analysis; Marker; Permeabilization; Probe; Stage; State

Indexed keywords

BIOLOGICAL MARKER; CCNA2 PROTEIN, HUMAN; CCNB1 PROTEIN, HUMAN; CYCLIN A2; CYCLIN B1; DNA;

BOOK; CELL LINE; CYTOLOGY; ENDOTHELIUM CELL; HUMAN; LASER SCANNING CYTOMETRY; METABOLISM; METHODOLOGY; MITOSIS; STAINING; VASCULAR ENDOTHELIUM;

BIOLOGICAL MARKERS; CELL LINE, TRANSFORMED; CYCLIN A2; CYCLIN B1; DNA; ENDOTHELIAL CELLS; ENDOTHELIUM, VASCULAR; HUMANS; LASER SCANNING CYTOMETRY; MITOSIS; STAINING AND LABELING;

EID: 79959579470     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-374912-3.00014-6     Document Type: Book

References (74)

1. Akimitsu N., Adachi N., Hirai H., Hossain M.S., Hamamoto H., Kobayashi M., Aratani Y., Koyama H., Sekimizu K. Enforced cytokinesis without complete nuclear division in embryonic cells depleting the activity of DNA topoisomerase IIalpha. Genes Cells 2003, 8:393-402.
2. Bauer K.D., Jacobberger J.W. Analysis of Intracellular Proteins. Methods Cell Biol. 1994, 41:351-376.
3. Baumgarth N., Roederer M. A practical approach to multicolor flow cytometry for immunophenotyping. J. Immunol. Methods 2000, 243:77-97.
4. Bozhkova V.P., Rott N.N., Chailakhian L.M. Relationship between the phases of mitosis in the cells of chimeric loach embryos. Ontogenez. 1978, 9:296-300.
5. Chakrabarti R., Jones J.L., Oelschlager D.K., Tapia T., Tousson A., Grizzle W.E. Phosphorylated LIM kinases colocalize with gamma-tubulin in centrosomes during early stages of mitosis. Cell Cycle 2007, 6:2944-2952.
6. Chen E., Huang X., Zheng Y., Li Y.J., Chesney A., Ben-David Y., Yang E., Hough M.R. Phosphorylation of HOX11/TLX1 on Threonine-247 during mitosis modulates expression of cyclin B1. Mol. Cancer 2010, 9:246.
7. D'Amato F. Considerations on selective inhibition of various stages of mitosis. G Ital Chemioter. 1954, 1:129-134.
8. Darzynkiewicz Z., Crissman H., Jacobberger J.W. Cytometry of the cell cycle: cycling through history. Cytometry A 2004, 58:21-32.
9. Darzynkiewicz Z. There's more than one way to skin a cat: yet another way to assess mitotic index by cytometry. Cytometry A 2008, 73:386-387.
10. del Campo A., Gimenez-Martin G., Lopez-Saez J.F., de la Torre C. Frailty of two cell cycle checkpoints which prevent entry into mitosis and progression through early mitotic stages in higher plant cells. Eur. J. Cell. Biol. 1997, 74:289-293.
11. Delone N.L., Vysotskii V.G. Sensitivity to acceleration of various phases of mitosis in the microspores of Tradescantia paludosa. Izv. Akad. Nauk. SSSR Biol. 1964, 12:900-907.
12. Deptala A., Bedner E., Gorczyca W., Darzynkiewicz Z. Activation of nuclear factor kappa B (NF-kappaB) assayed by laser scanning cytometry (LSC). Cytometry 1998, 33:376-382.
13. Dobrokhotov V.N., Valvas V.S. Dynamics of the phases of mitosis and the duration of cell division. Biull. Eksp. Biol. Med. 1981, 92:76-78.
14. Dodson H., Bourke E., Jeffers L.J., Vagnarelli P., Sonoda E., Takeda S., Earnshaw W.C., Merdes A., Morrison C. Centrosome amplification induced by DNA damage occurs during a prolonged G2 phase and involves ATM. EMBO J. 2004, 23:3864-3873.
15. el-Alfy M., Leblond C.P. Long duration of mitosis and consequences for the cell cycle concept, as seen in the isthmal cells of the mouse pyloric antrum. II. Duration of mitotic phases and cycle stages, and their relation to one another. Cell Tissue Kinet. 1987, 20:215-226.
16. Fortunato E.A., Sanchez V., Yen J.Y., Spector D.H. Infection of cells with human cytomegalovirus during S phase results in a blockade to immediate - early gene expression that can be overcome by inhibition of the proteasome. J. Virol. 2002, 76:5369-5379.
17. Frisa P.S., Jacobberger J.W. Cell cycle-related cyclin b1 quantification. PLoS One. 2009, 4:e7064.
18. Gorczyca W., Smolewski P., Grabarek J., Ardelt B., Ita M., Melamed M.R., Darzynkiewicz Z. Morphometry of nucleoli and expression of nucleolin analyzed by laser scanning cytometry in mitogenically stimulated lymphocytes. Cytometry. 2001, 45:206-213.
19. Gross W.O. Mitosis. demonstration of separate phases. Verh. Anat. Ges. 1975, 69:851-854.
20. Grosset L., Odartchenko N. Duration of mitosis and separate mitotic phases compared to nuclear DNA content in erythroblasts of four vertebrates. Cell. Tissue Kinet. 1975, 8:91-96.
21. Guarguaglini G., Duncan P.I., Stierhof Y.D., Holmstrom T., Duensing S., Nigg E.A. The forkhead-associated domain protein Cep170 interacts with Polo-like kinase 1 and serves as a marker for mature centrioles. Mol. Biol. Cell. 2005, 16:1095-1107.
22. Haider A.S., Grabarek J., Eng B., Pedraza P., Ferreri N.R., Balazs E.A., Darzynkiewicz Z. In vitro model of" wound healing" analyzed by laser scanning cytometry: accelerated healing of epithelial cell monolayers in the presence of hyaluronate. Cytometry A. 2003, 53:1-8.
23. Hara M., Igarashi J., Yamashita K., Iigo M., Yokosuka M., Ohtani-Kaneko R., Hirata K., Herbert D.C. Proteins recognized by antibodies against isolated cytological heterochromatin from rat liver cells change their localization between cell species and between stages of mitosis (interphase vs metaphase). Tissue Cell. 1999, 31:505-513.
24. Hong K.U., Choi Y.B., Lee J.H., Kim H.J., Kwon H.R., Seong Y.S., Kim H.T., Park J., Bae C.D., Hong K.M. Transient phosphorylation of tumor associated microtubule associated protein (TMAP)/cytoskeleton associated protein 2 (CKAP2) at Thr-596 during early phases of mitosis. Exp] Mol. Med. 2008, 40:377-386.
25. Hozak P., Zatsepina O., Vasilyeva I., Chentsov Y. An electron microscopic study of nucleolus - organizing regions at some stages of the cell cycle (G0 period, G2 period, mitosis). Biol. Cell. 1986, 57:197-205.
26. Huang X., Kurose A., Tanaka T., Traganos F., Dai W., Darzynkiewicz Z. Sequential phosphorylation of Ser-10 on histone H3 and ser-139 on histone H2AX and ATM activation during premature chromosome condensation: relationship to cell-cycle phase and apoptosis. Cytometry A. 2006, 69:222-229.
27. Jacobberger J.W. Flow cytometric analysis of intracellular protein epitopes. Immunophenotyping 2000, 361-405. Wiley-Liss, New York. C.A. Stewart, J.K.A. Nicholson (Eds.).
28. Jacobberger J.W. Stoichiometry of immunocytochemical staining reactions. Methods Cell Biol. 2001, 63:271-298.
29. Jacobberger J.W., Frisa P.S., Sramkoski R.M., Stefan T., Shults K.E., Soni D.V. A new biomarker for mitotic cells. Cytometry A. 2008, 73:5-15.
30. Jacobberger J.W., Sramkoski R.M., Stefan T. Multiparameter cell cycle analysis. Flow Cytometry Protocols, Methods in Molecular Biology 2011, Vol. 699. Springer Science, New York, p. 21.
31. Jacobberger J.W., Sramkoski R.M., Zhang D., Zumstein L.A., Doerksen L.D., Merritt J.A., Wright S.A., Shults K.E. Bivariate analysis of the p53 pathway to evaluate Ad-p53 gene therapy efficacy. Cytometry 1999, 38:201-213.
32. Juan, G., Darzynkiewicz, Z., 2004. Detection of mitotic cells. Curr Protoc Cytom. Chapter 7, Unit 7 24.
33. Kamentsky L.A. Laser scanning cytometry. Methods Cell Biol. 2001, 63:51-87.
34. Kamentsky L.A., Kamentsky L.D. Microscope-based multiparameter laser scanning cytometer yielding data comparable to flow cytometry data. Cytometry. 1991, 12:381-387.
35. Kammerer B.D., Sardella B.A., Kultz D. Salinity stress results in rapid cell cycle changes of tilapia (Oreochromis mossambicus) gill epithelial cells. J. Exp. Zool. A Ecol. Genet. Physiol. 2009, 311:80-90.
36. Kawamura K., Fujikawa-Yamamoto K., Ozaki M., Iwabuchi K., Nakashima H., Domiki C., Morita N., Inoue M., Tokunaga K., Shiba N., Ikeda R., Suzuki K. Centrosome hyperamplification and chromosomal damage after exposure to radiation. Oncology. 2004, 67:460-470.
37. Kawamura K., Morita N., Domiki C., Fujikawa-Yamamoto K., Hashimoto M., Iwabuchi K., Suzuki K. Induction of centrosome amplification in p53 siRNA-treated human fibroblast cells by radiation exposure. Cancer Sci. 2006, 97:252-258.
38. Kurose A., Yoshida W., Yoshida M., Sawai T. Effects of paclitaxel on cultured synovial cells from patients with rheumatoid arthritis. Cytometry. 2001, 44:349-354.
39. Kuz'mina S.V. The ratio of the phases of mitosis in decontaminated cell lines and in cell lines contaminated with mycoplasma. Biull. Eksp. Biol. Med. 1972, 13:100-102.
40. Langendorff H., Hagen U., Ernst H. Studies on a biological radiation protection. XXVIII. Effect of cysteamine on various stages of the mitosis cycle. Strahlentherapie 1958, 107:574-579.
41. Leblond C.P., El-Alfy M. The eleven stages of the cell cycle, with emphasis on the changes in chromosomes and nucleoli during interphase and mitosis. Anat. Rec. 1998, 252:426-443.
42. Lopes C.S., Sampaio P., Williams B., Goldberg M., Sunkel C.E. The Drosophila Bub3 protein is required for the mitotic checkpoint and for normal accumulation of cyclins during G2 and early stages of mitosis. J. Cell. Sci. 2005, 118:187-198.
43. Luther E., Kamentsky L.A. Resolution of mitotic cells using laser scanning cytometry. Cytometry. 1996, 23:272-278.
44. Mak S.K., Kultz D. Gadd45 proteins induce G2/M arrest and modulate apoptosis in kidney cells exposed to hyperosmotic stress. J. Biol. Chem. 2004, 279:39075-39084.
45. Manton I., Kowallik K., von Stosch H.A. Observations on the fine structure and development of the spindle at mitosis and meiosis in a marine centric diatom (Lithodesmium undulatum). II. The early meiotic stages in male gametogenesis. J. Cell. Sci. 1969, 5:271-298.
46. Manton I., Kowallik K., von Stosch H.A. Observations on the fine structure and development of the spindle at mitosis and meiosis in a marine centric diatom (Lithodesmium undulatum). 3. The later stages of meiosis I in male gametogenesis. J. Cell Sci. 1970, 6:131-157.
47. Maruvada P., Dmitrieva N.I., East-Palmer J., Yen P.M. Cell cycle-dependent expression of thyroid hormone receptor-beta is a mechanism for variable hormone sensitivity. Mol. Biol. Cell. 2004, 15:1895-1903.
48. Mercie P., Belloc F., Bihlou-Nabera C., Barthe C., Pruvost A., Renard M., Seigneur M., Bernard P., Marit G., Boisseau M.R. Comparative methodologic study of NFkappaB activation in cultured endothelial cells. J. Lab. Clin. Med. 2000, 136:402-411.
49. Ohshima S. Abnormal mitosis in hypertetraploid cells causes aberrant nuclear morphology in association with H2O2-induced premature senescence. Cytometry A. 2008, 73:808-815.
50. Ohshima S., Seyama A. Cellular aging and centrosome aberrations. Ann. N.Y. Acad. Sci. 2010, 1197:108-117.
51. Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci. Signal. 2010, 3:ra3.
52. Pachmann K., Zhao S., Schenk T., Kantarjian H., El-Naggar A.K., Siciliano M.J., Guo J.Q., Arlinghaus R.B., Andreeff M. Expression of bcr-abl mRNA in individual chronic myelogenous leukaemia cells as determined by in situ amplification. Br. J. Haematol. 2001, 112:749-759.
53. Perez-Cadahia B., Drobic B., Davie J.R. H3 phosphorylation: dual role in mitosis and interphase. Biochem. Cell. Biol. 2009, 87:695-709.
54. Peters J.M. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol. 2006, 7:644-656.
55. Pham N.A., Jacobberger J.W., Schimmer A.D., Cao P., Gronda M., Hedley D.W. The dietary isothiocyanate sulforaphane targets pathways of apoptosis, cell cycle arrest, and oxidative stress in human pancreatic cancer cells and inhibits tumor growth in severe combined immunodeficient mice. Mol. Cancer Ther. 2004, 3:1239-1248.
56. Pines J. Mitosis: a matter of getting rid of the right protein at the right time. Trends Cell. Biol. 2006, 16:55-63.
57. Pines J., Rieder C.L. Re-staging mitosis: a contemporary view of mitotic progression. Nat. Cell. Biol. 2001, 3:E3-6.
58. Rosdahl I.K., Lindstrom S. Morphology of epidermal melanocytes in different stages of mitosis. Acta Derm. Venereol. 1980, 60:209-215.
59. Santamaria A., Neef R., Eberspacher U., Eis K., Husemann M., Mumberg D., Prechtl S., Schulze V., Siemeister G., Wortmann L., Barr F.A., Nigg E.A. Use of the novel Plk1 inhibitor ZK-thiazolidinone to elucidate functions of Plk1 in early and late stages of mitosis. Mol. Biol. Cell. 2007, 18:4024-4036.
60. Schwartz J.L., Muscat J.E., Baker V., Larios E., Stephenson G.D., Guo W., Xie T., Gu X., Chung F.L. Oral cytology assessment by flow cytometry of DNA adducts, aneuploidy, proliferation and apoptosis shows differences between smokers and non-smokers. Oral. Oncol. 2003, 39:842-854.
61. Schwock J., Ho J.C., Luther E., Hedley D.W., Geddie W.R. Measurement of signaling pathway activities in solid tumor fine-needle biopsies by slide-based cytometry. Diagn. Mol. Pathol. 2007, 16:130-140.
62. Siracky J., Sparrow S., Jones M. Changes in distribution of the phases of mitosis in human tumor xenografts following drug treatment. Neoplasma. 1984, 31:539-544.
63. Soerenby L., Lindahl P.E. On the concentrating of ascites tumour cells in stages of pre-mitosis and mitosis by counter-streaming contrifugation. Exp. Cell Res. 1964, 35:214-217.
64. Soni D.V., Jacobberger J.W. Inhibition of cdk1 by alsterpaullone and thioflavopiridol correlates with increased transit time from mid G2 through prophase. Cell Cycle. 2004, 3:349-357.
65. Soni D.V., Sramkoski R.M., Lam M., Stefan T., Jacobberger J.W. Cyclin B1 is rate limiting but not essential for mitotic entry and progression in mammalian somatic cells. Cell. Cycle. 2008, 7:1285-1300.
66. Sramkoski R.M., Wormsley S.W., Bolton W.E., Crumpler D.C., Jacobberger J.W. Simultaneous detection of cyclin B1, p105, and DNA content provides complete cell cycle phase fraction analysis of cells that endoreduplicate. Cytometry. 1999, 35:274-283.
67. Starosvetskaia N.A. Ultrastructural characteristics of the final stages of mitosis in tissue culture cells. Vestn. Akad. Med. Nauk. SSSR. 1970, 25:51-56.
68. Walter J., Schermelleh L., Cremer M., Tashiro S., Cremer T. Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages. J. Cell. Biol. 2003, 160:685-697.
69. Weicker H. Erythroblastic mitosis; duration of mitosis and course of phases of the hemi-homoplastic karyokinesis of the proerythroblasts and of the three erythroblast maturation divisions. Z. Klin. Med. 1954, 151:407-428.
70. Yamamoto Y., Matsuyama H., Kawauchi S., Matsumoto H., Nagao K., Ohmi C., Sakano S., Furuya T., Oga A., Naito K., Sasaki K. Overexpression of polo-like kinase 1 (PLK1) and chromosomal instability in bladder cancer. Oncology. 2006, 70:231-237.
71. Zak M. Changes i the mitotic activity and the portion of single mitosis-phases of the bone marrow cells after x-ray irradiation and administration of chloroquine. Strahlentherapie 1970, 139:587-592.
72. Zak M., Cerny M. A method for the evaluation of the mitotic index and the individual phases of mitosis in bone marrow cells using hypotonized cells. Cas Lek Cesk. 1966, 105:1178-1179.
73. Zatsepina O., Hozak P., Babadjanyan D., Chentsov Y. Quantitative ultrastructural study of nucleolus-organizing regions at some stages of the cell cycle (G0 period, G2 period, mitosis). Biol. Cell. 1988, 62:211-218.
74. Zosimovskaia A.I., Liapunova N.A. The duration of mitosis and stages of the mitotic cycle in primary culture of human embryonic fibroblasts. Tsitologiia. 1966, 8:208-215.