Expression of bcr-abl mRNA in individual chronic myelogenous leukaemia cells as determined by in situ amplification

British Journal of Haematology

Volumn 112, Issue 3, 2001, Pages 749-759

  Pachmann, Katharina ^a   Zhao, Shourong ^a   Schenk, Thomas ^a   Kantarjian, Hagop ^a   El Naggar, Adel K ^a   Siciliano, Michael J ^a   Guo, Jie Qiang ^a   Arlinghaus, Ralph B ^a   Andreeff, Michael ^a  

^a UNIVERSITY OF TEXAS   (United States)

Author keywords

bcr abl mRNA; CML; In situ amplification

Indexed keywords

ALPHA INTERFERON; ANTINEOPLASTIC AGENT; BCR ABL PROTEIN; CYTARABINE; FLUOROCHROME; GRANULOCYTE COLONY STIMULATING FACTOR; HYDROXYUREA; MESSENGER RNA;

ARTICLE; AUTOMATION; BLAST CELL CRISIS; CHRONIC MYELOID LEUKEMIA; CLINICAL ARTICLE; CORRELATION FUNCTION; CYTOGENETICS; CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; GENE AMPLIFICATION; GENE EXPRESSION; HUMAN; HUMAN CELL; IMAGE ANALYSIS; INTERPHASE; MOLECULAR GENETICS; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ANALYSIS OF VARIANCE; BLOTTING, WESTERN; FUSION PROTEINS, BCR-ABL; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IN SITU HYBRIDIZATION, FLUORESCENCE; LEUKEMIA, MYELOID, CHRONIC; METAPHASE; REMISSION INDUCTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TUMOR CELLS, CULTURED;

EID: 0035107533     PISSN: 00071048     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2141.2001.02510.x     Document Type: Article

References (51)

1. A correlation between the expression of the bcr-abl chimeric gene and severity of the clinical state of CML patients with time
2. Inhibition of apoptosis by BCR-ABL in chronic myeloid leukemia
3. Detection of chimeric BCR-ABL gene on bone marrow samples and blood smears in chronic myeloid and acute lymphoblastic leukemia by in situ hybridizytion
4. T-cell immunity to oncogenic proteins including mutated ras and chimeric bcr/abl
5. Use of leukemic dendritic cells for the generation of antileukemic cellular cytotoxicity against Philadelphia chromosome positive chronic myelogenous leukemia
6. Structural and signalling requirements for BCR-ABL mediated transformation and inhibition of apoptosis
7. Minimal residual disease after bone marrow transplant for chronic myeloid leukemia detected by the polymerase chain reaction
8. Animal models of BCR/ABL-induced leukemia
9. IL-2 inhibits proliferation of K562 cells and reduces accumulation of bcr/abl mRNA and oncoprotein
10. In-cell PCR from mRNA: Amplifying and linking the rearranged immunoglobulin heavy and light V-genes within single cells
11. Polymerase chain reaction: A method for monitoring tumor cell purge by long-term culture in BCR/ABL positive acute lymphoblastic leukemia
12. Cytotoxic T cells overcome BCR-ABL mediated resistance to apoptosis
13. Increase in bcr/abl chimeric mKNa expression in tumor cells of patients with chronic myeloid leukemia precedes disease progression
14. Optimizing tratment for chronic myeloid leukemia
15. Philadephia chromosome breakpoints are clustered within a limited region, bcr, on chromosome 22
16. BCR-ABL protein expression in peripheral blood cells of chronic myelogenous leukemia patient undergoing therapy
17. Structural organization of the bcr gene and its role in the Ph1 translocation
18. Variable numbers of transcripts persist in CML patients who achieve complete cytogenetic remission with interferon-alpha
19. DNA methylation changes in hematologic malignancies: Biologic and clinical implications
20. A temperature sensitive p210 BCR-ABL mutant defines the primary consequences of BCR-ABL tyrosine kinase expression in growth factor dependent cells
21. Investigation of bcr/abl transcription by Ph-positive chronic myeloid leukemia progenitors
22. Variable transcription of BCR/ABL by Ph+ cells arising from hematopoietic progenitors in chronic myeloid leukemia
23. The human cellular abl gene product in the chronic myelogenous leukemia
24. Lymphokine activated killer cells can discriminate CD34+ leukemai cells from normal hematopoietic progenitor cells
25. Kinetics of increasing BCR-ABL transcript numbers in chronic myeloid leukemia patients who relapse after bone marrow transplantation
26. BCR-ABL tyrosin kinase is autophosphorylated or transphosphorylates P160 BCR on tyrosine predominantly within the first BCR exon
27. Inhibition of BCR serine kinase by tyrosine phosphorylation
28. BCR-ABL maintains resistance in chronic myelogenous leukemia cells to apoptotic cell death
29. A coiled-coil domain of Bcr is essential for the transforming function of Bcr-Abl oncoproteins
30. Minimal residual disease after bone marrow transplantation for chronic myelogenous leukemia and implications for graft-versus leukemia effect: A review of recent results
31. Karyotypic and clinical progression in chronic myelogenous leukemia after 30 years
32. Modified histogram subtraction technique for analysis of flow cytometry data
33. In situ amplification of rearranged gene sequences in individual cells for tracing clonal cells in smears from blood and bone marrow
34. In situ amplification of BCR/ABL sequences for analysis of mRNA expression at the single cell level in chronic myeloid leukaemia (CML)
35. Interferon-alpha 2a therapy in CML disappearance of BCR/ABL transcript in a case of longlasting continuous cytogenetic conversion
36. The Philadelphia chromosome and chronic myelogenous leukemia
37. BCR-ABL induced oncogenesis is mediated by direct interaction with the SH2 domain of the GRB-2 adaptor protein
38. Bcr/abl oncoproteins bind directly to activators of the Ras signalling pathway
39. A new consistent chromosomal abnormality in chronic myelogenous leukemia identified by quinacrine fluorescence and Giemsa staining
40. The proto-oncogene product p120abl and the adaptor proteins CRKL and c-CRK link cABL, p190bcr-abl and p210bcr-abl to the phosphatiylinositol-3′ kinase pathway
41. Multilineage involvement of Philadelphia chromosome positive acute lymphoblastic leukemia
42. Detection of bcr/abl mRNA in single progenitor colonies from patients with chronic myeloid leukemia by PCR: Comparison with cytogenetics and PCR from uncultured cells
43. Hypermetaphase FISH for quantitative monitoring of Ph-positive cells in patients with CML during treatment
44. Improved sensitivity of BCR-ABL detection: A triple probe three color fluorescence in situ hybridization system
45. Long term culture and molecular biology studies highlight differences in relative BCR-ABL expression levels in the peripheral blood and bone marrow of a patient with chronic granulocytic leukemia
46. In situ amplification of single copy gene segments in individual cells by the polymerase chain reaction
47. Tyrosine phosphorylation of CRKL in Philadelphia + leukemia
48. A new method of 'in-cell reverse transcriptase polymerase chain reaction' for the detection of BCR/ABL transcript in chronic myeloid leukemia
49. Molecular analysis of hematopoietic colonies derived from chronic myeloid leukemia patients: Interphase fluorescence in situ hybridization compared with RT-PCR
50. Detection of bcr-abl fusion in chronic myelogenous leukemia by in situ hybridization
51. Detection of tumor specific antigens in Philadelphia chromosome positive leukemias