In Vitro Model of "Wound Healing" Analyzed by Laser Scanning Cytometry: Accelerated Healing of Epithelial Cell Monolayers in the Presence of Hyaluronate

Cytometry Part A

Volumn 53, Issue 1, 2003, Pages 1-8

  Haider, Asifa S ^a   Grabarek, Jerzy ^a   Eng, Ben ^a   Pedraza, Paulina ^a   Ferreri, Nicholas R ^a   Balazs, Endre A ^b   Darzynkiewicz, Zbigniew ^a  

^a NEW YORK MEDICAL COLLEGE   (United States)

^b Matrix Biology Institute   (United States)

Author keywords

Apoptosis; Bromodeoxyuridine incorporation; Caspase activation; Cell cycle; Cell migration; Epithelial cells; Fluorochrome labeled inhibitor of caspases; Hyaluronate; Hyaluronic acid; Laser scanning cytometry

Indexed keywords

BENZYLOXYCARBONYLVALYLALANYLASPARTYL FLUOROMETHYL KETONE; BROXURIDINE; CASPASE; CASPASE INHIBITOR; HYALURONIC ACID;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CULTURE; CELL CYCLE G1 PHASE; CELL CYCLE S PHASE; CELL MIGRATION; CELL PROLIFERATION; CELL SHAPE; CELL SHRINKAGE; CELLULAR DISTRIBUTION; CHROMATIN CONDENSATION; CINERADIOGRAPHY; CONTROLLED STUDY; CYTOMETRY; ENZYME ACTIVATION; EPITHELIUM CELL; EXTRACELLULAR MATRIX; IN VITRO STUDY; INFLAMMATION; KIDNEY EPITHELIUM; LASER MICROSCOPY; LASER SCANNING CYTOMETRY; MALE; MICROSCOPY; MITOGENESIS; MITOSIS; MULTIPARAMETER ANALYSIS; NONHUMAN; PARAMETER; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RAT; SIGNAL TRANSDUCTION; STATISTICAL ANALYSIS; TIME LAPSE PHOTOGRAPHY; WOUND; WOUND CLOSURE; WOUND HEALING;

EID: 0346124150     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.a.10032     Document Type: Article

References (28)

1. Ronot X, Doisy A, Tracqui P. Quantitative study of dynamic behavior of cell monolayers during in vitro wound healing by optical flow analysis. Cytometry 2000;41:19-30.
2. Gottrup F, Agren MS, Karismark T. Models for use in wound healing research: a survey focusing on in vitro and in vivo adult soft tissue. Wound Repair Regen 2000;8:83-96.
3. Watson AL, Palmer ME, Burton GJ. An in vitro model for the study of wound healing in first trimester human placenta. Cell Tissue Res 1996;286:431-438.
4. Lin CP, Boehnke M. A new model for in vitro corneal epithelial wound healing study. Kaohsiung J Med Sci 1997;13:475-479.
5. Kuhn MA, Smith PD, Hill DP, Ko F, Meltzer DD, Vande Berg JS, Robson MC. In vitro fibroblast populated collagen lattices are not good models of in vivo clinical wound healing. Wound Repair Regen 2000;8:270-276.
6. Zahm JM, Kaplan H, Herard AL, Doriot F, Pierrot D, Somelette P, Puchelle E. Cell migration and proliferation during the in vitro wound repair of the respiratory epithelium. Cell Motil Cytoskeleton 1997;37:33-43.
7. Kamentsky LA. Laser scanning cytometry. Methods Cell Biol 2001;63:51-87.
8. Darzynkiewicz Z, Bedner E, Li X, Gorczyca W, Melamed MR. Laser scanning cytometry. A new instrumentation with many applications. Exp Cell Res 1999;249:1-12.
9. Balazs EA. The viscoelastic intercellular matrix and control of cell function by hyaluronan. In: Laurent TC, editor. The chemistry, biology and medical applications of hyaluronan and its derivatives. London: Portland Press; 1998. p 185-204.
10. Laurent TC, editor. The chemistry, biology and medical applications of hyaluronan and its derivatives. London: Portland Press; 1998.
11. Wang D, An S-J, Wang W-H, McGiff JC, Ferreri NR. CaR-mediated COX-2 expression in primary cultured mTAL cells. Am J Physiol Renal Physiol 2001;281:F658-F664.
12. Smolewski P, Grabarek J, Phelps DJ, Darzynkiewicz Z. Stathmo-apoptosis: arresting apoptosis by fluorochrome-labeled inhibitor of caspases. Int J Oncol 2001;19:657-663.
13. Bedner E, Smolewski P, Amstad P, Darzynkiewicz Z. Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA): correlation with DNA fragmentation. Exp Cell Res 2000;260:308-313.
14. Smolewski P, Bedner E, Du L, Hsieh T-C, Wu J, Phelps DJ, Darzynkiewicz Z. Detection of caspases activation by fluorochrome-labeled inhibitors: multiparameter analysis by laser scanning cytometry. Cytometry 2001;44:73-82.
15. Garcia-Calvo, M, Peterson EP, Leiting B, Ruel R, Nicholson DW, Thornberry NA. Inhibition of human caspases by peptide-based and macromolecular inhibitors. J Biol Chem 1998;273:32608-32613.
16. Thornberry NA, Rano TA, Peterson EP, Rasper DM, Timkey T, Garcia-Calvo M Houtzager VM, Nordstrom PA, Roy S, Valliancourt JP, Chapman KT, Nicholson DW: A combinatorial approach defines specificities of members of the caspase family and granzyme B. J Biol Chem 1997;272:17907-17911.
17. Darzynkiewicz Z, Bruno S, Delbino G, Gorczyca W, Hotz MA, Lassota P, Traganos F. Features of apoptotic cells measured by flow cytometry. Cytometry 1992;13:795-808.
18. Darzynkiewicz Z, Juan G, Li X, Murakami T, Traganos F. Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis). Cytometry 1997;27:1-20.
19. Sugiyama T, Miyauchi S, Machida A, Miyazaki K, Tokuyasu K, Nakazawa K. The effect of sodium hyaluronate on the migration of rabbit corneal epithelium. II. The effect of topical administration. J Ocul Pharmacol Ther 1991;7:53-64.
20. Miyazaki T, Miauchi S, Nakamure T, Takeshita S, Horie K. The effect of sodium hyaluronate on the growth of rabbit corneal epithelial cells in vitro. J Ocul Pharmacol Ther 1996;12:409-415.
21. Takahashi K, Takeuchi J, Takahashi T, Miauchi S, Horie K, Uchiyama Y. Effects of sodium hyaluronate on epithelial healing of vesical mucosa and vesical fibrosis in rabbits with acetic acid induced cystitis. J Urol 2001;166:710-713.
22. Longaker MT, Chiu ES, Adzick NS, Stern M, Harrison MR, Stern R. Studies in fetal wound healing. V. A prolonged presence of hyaluronic acid characterized fetal wound fluid. Ann Surg 1991;213:292-296.
23. Larsen NE, Lombard K, Parent EG, Balazs EA. Effect of hylan on cartilage and chondrocyte cultures. J Orthop Res 1992;10:23-32.
24. Docherty R, Forrester JV, Lackie JM, Gregory DW. Glycosaminoglycans facilitate the movement of fibroblasts through three-dimensional collagen matrices. J Cell Sci 1989;92:263-270.
25. Johns DB, Rodgers KE, Donahue WD, Kiopers TC, diZerega GS. Reduction of adhesion formation by postoperative administration of ionically cross-linked hyaluronic acid. Fertil Steril 1997;68:37-42.
26. Denlinger JL. Hyaluronan and its derivatives as viscoelastics in medicine. In: Laurent TC, editor. The chemistry, biology and medical applications of hyaluronan and its derivatives. London: Portland Press; 1998. p 235-242.
27. Reijnem MM, Falk P, van Goor H, Holmdahl. The antiadhesive agent sodium hyaluronate increases the proliferation rate of human peritoneal mesothelial cells. Fertil Steril 2000;74:146-151.
28. Hanada K, Shizamaki J, Shimmura S, Tsubota K. Multilayered anmiotic membrane transplantation for severe ulceration of the cornea and sclera. Am J Ophthalmol 2001;131:324-331.