Optimized generation of vectors for the construction of Haloferax volcanii deletion mutants

Journal of Microbiological Methods

Volumn 75, Issue 2, 2008, Pages 201-204

  Hammelmann, M ^a   Soppa, Jörg ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

Deletion mutant; Fusion PCR; Haloferax volcanii; Restriction selection cloning; sRNA

Indexed keywords

PLASMID VECTOR; RESTRICTION ENDONUCLEASE; TRANSFER RNA;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; CLONING; DELETION MUTANT; GENE DELETION; GENE FUSION; HALOFERAX VOLCANII; NONHUMAN; PLASMID; PLASMID MH101; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION SELECTION CLONING;

DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; GENE DELETION; GENETIC TECHNIQUES; GENETIC VECTORS; HALOFERAX VOLCANII; MUTATION; PLASMIDS; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; RNA, ARCHAEAL;

ARCHAEA; HALOFERAX VOLCANII;

EID: 50549098705     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2008.05.029     Document Type: Article

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