Specificity of HCPTP variants toward EphA2 tyrosines by quantitative selected reaction monitoring

Protein Science

Volumn 20, Issue 7, 2011, Pages 1172-1181

  Balasubramaniam, Deepa ^a   Paul, Lake N ^b   Homan, Kristoff T ^a   Hall, Mark C ^b,c   Stauffacher, Cynthia V ^a,b,c  

^a PURDUE UNIVERSITY   (United States)

^b PURDUE UNIVERSITY   (United States)

^c Purdue University   (United States)

Author keywords

EphA2 receptor tyrosine kinase; Human cytoplasmic protein tyrosine phosphatase; Phosphotyrosine signaling; Selected reaction monitoring

Indexed keywords

EPHRIN A1; EPHRIN RECEPTOR A2; LIGAND; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHOTYROSINE; PROTEIN TYROSINE PHOSPHATASE; TYROSINE; TYROSINE KINASE RECEPTOR;

ARTICLE; CONTROLLED STUDY; CYTOPLASM; ENZYME ACTIVITY; ENZYME SPECIFICITY; HUMAN; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN DEPHOSPHORYLATION; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN VARIANT; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; HUMANS; MOLECULAR SEQUENCE DATA; PHOSPHOPEPTIDES; PHOSPHORYLATION; PROTEIN ISOFORMS; PROTEIN STRUCTURE, TERTIARY; PROTEIN TYROSINE PHOSPHATASES; PROTO-ONCOGENE PROTEINS; RECEPTOR, EPHA2; RECOMBINANT PROTEINS;

MAMMALIA;

EID: 79959365279     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.646     Document Type: Article

References (32)

1. Walker-Daniels J, Hess A, Hendrix M, Kinch M (2003) Differential regulation of EphA2 in normal and malignant cells. Am J Pathol 162:1037-1042. (Pubitemid 36350648)
2. Ireton RC, Chen J (2005) EphA2 receptor tyrosine kinase as a promising target for cancer therapeutics. Curr Cancer Drug Targets 5:149-157. (Pubitemid 40694857)
3. Kinch MS, Carles-Kinch K (2003) Overexpression and functional alterations of the EphA2 tyrosine kinase in cancer. Clin Exp Metastasis 20:59-68. (Pubitemid 36308137)
4. Zantek ND, Azimi M, Fedor-Chaiken M, Wang B, Brackenbury R, Kinch MS (1999) E-Cadherin regulates the function of the EphA2 receptor tyrosine kinase 1. Cell Growth Differ 10:629-638. (Pubitemid 29455727)
5. Zelinski DP, Zantek ND, Stewart JC, Irizarry AR, Kinch MS (2001) EphA2 overexpression causes tumorigenesis of mammary epithelial cells. Cancer Res 61:2301-2306. (Pubitemid 32692063)
6. Carles-Kinch K, Kilpatrick KE, Stewart JC, Kinch MS (2002) Antibody targeting of the EphA2 tyrosine kinase inhibits malignant cell behavior. Cancer Res 62:2840-2847. (Pubitemid 34525769)
7. Zhou R (1998) The Eph family receptors and ligands. Pharmacol Ther 77:151-181.
8. Himanen JP, Nikolov DB (2003) Eph signaling: a structural view. Trends Neurosci 26:46-51.
9. Kikawa KD, Vidale DR, Van Etten RL, Kinch MS (2002) Regulation of the EphA2 kinase by the low molecular weight tyrosine phosphatase induces transformation. J Biol Chem 277:39274-39279. (Pubitemid 35190898)
10. Chiarugi P, Taddei ML, Schiavone N, Papucci L, Giannoni E, Fiaschi T, Capaccioli S, Raugei G, Ramponi G (2004) LMW-PTP is a positive regulator of tumor onset and growth. Oncogene 23:3905-3914. (Pubitemid 38747919)
11. Parri M, Buricchi F, Taddei ML, Giannoni E, Raugei G, Ramponi G, Chiarugi P (2005) EphrinA1 repulsive response is regulated by an EphA2 tyrosine phosphatase. J Biol Chem 280:34008-34018. (Pubitemid 41443122)
12. Fang WB, Ireton RC, Zhuang G, Takahashi T, Reynolds A, Chen J (2008) Overexpression of EPHA2 receptor destabilizes adherens junctions via a RhoA-dependent mechanism. J Cell Sci 121:358-368. (Pubitemid 351356707)
13. Fang WB, Brantley-Sieders DM, Hwang Y, Ham A-JL, Chen J (2008) Identification and functional analysis of phosphorylated tyrosine residues within EphA2 receptor tyrosine kinase. J Biol Chem 283:16017-16026.
14. Oppermann FS, Gnad F, Olsen JV, Hornberger R, Greff Z, Kéri G, Mann M, Daub H (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8:1751-1764.
15. Binns KL, Taylor PP, Sicheri F, Pawson T, Holland SJ (2000) Phosphorylation of tyrosine residues in the kinase domain and juxtamembrane region regulates the biological and catalytic activities of Eph receptors. Mol Cell Biol 20:4791-4805. (Pubitemid 30396126)
16. Leone M, Cellitti J, Pellecchia M (2008) NMR studies of a heterotypic Sam-Sam domain association: the interaction between the lipid phosphatase Ship2 and the EphA2 receptor. Biochemistry 47:12721-12728.
17. Wo YY, McCormack AL, Shabanowitz J, Hunt DF, Davis JP, Mitchell GL, Van Etten RL (1992) Sequencing, cloning, and expression of human red cell-type acid phosphatase, a cytoplasmic phosphotyrosyl protein phosphatase. J Biol Chem 267:10856-10865.
18. Zabell APR, Schroff AD, Jr, Bain BE, Van Etten RL, Wiest O, Stauffacher CV (2006) Crystal structure of the human B-form low molecular weight phosphotyrosyl phosphatase at 1.6 Å resolution. J Biol Chem 281:6520-6527. (Pubitemid 43847585)
19. Cirri P, Fiaschi T, Chiarugi P, Camici G, Manao G, Raugei G, Ramponi G (1996) The molecular basis of the differing kinetic behavior of the two low molecular mass phosphotyrosine protein phosphatase isoforms. J Biol Chem 271:2604-2607. (Pubitemid 26047870)
20. Homan K, Balasubramaniam D, Zabell A, Wiest O, Helquist P, Stauffacher C (2010) Identification of novel inhibitors for a low molecular weight protein tyrosine phosphatase via virtual screening. Bioorg Med Chem 18:5449-5456.
21. Zhang ZY, Dixon JE (1994) Protein tyrosine phosphatases: mechanism of catalysis and substrate specificity. Adv Enzymol Relat Areas Mol Biol 68:1-36.
22. Zhang M, Stauffacher CV, Lin D, Van Etten RL (1998) Crystal structure of a human low molecular weight phosphotyrosyl phosphatase. Implications for substrate specificity. J Biol Chem 273:21714-21720. (Pubitemid 28405346)
23. Zabell KM, Laurence JS, Kinch MS, Knapp DW, Stauffacher CV (2005) Expression and purification of the intact cytoplasmic domain of the human ephrin receptor A2 tyrosine kinase in Escherichia coli. Protein Expr Purif 47:210-216.
24. Rikova K, Guo A, Zeng Q, Possemato A, Yu J, Haack H, Nardone J, Lee K, Reeves C, Li Y, Hu Y, Tan Z, Stokes M, Sullivan L, Mitchell J, Wetzel R, MacNeill J, Ren JM, Yuan J, Bakalarski CE, Villen J, Kornhauser JM, Smith B, Li D, Zhou X, Gygi SP, Gu T-L, Polakiewicz RD, Rush J, Comb MJ (2007) Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. Cell 131:1190-1203. (Pubitemid 350235027)
25. Wegener AD, Jones LR (1984) Phosphorylation-induced mobility shift in phospholamban in sodium dodecyl sulfate-polyacrylamide gels. Evidence for a protein structure consisting of multiple identical phosphorylatable subunits. J Biol Chem 259:1834-1841. (Pubitemid 14146574)
26. Balasubramaniam D, Eissler C, Stauffacher C, Hall M (2010) Use of selected reaction monitoring data for label free quantification of protein modification stoichiometry. Proteomics 10:4301-4305.
27. Steen H, Jebanathirajah JA, Springer M, Kirschner MW (2005) Stable isotope-free relative and absolute quantitation of protein phosphorylation stoichiometry by MS. Proc Natl Acad Sci USA 102:3948-3953.
28. Koolpe M, Dail M, Pasquale EB (2002) An ephrin mimetic peptide that selectively targets the EphA2 receptor. J Biol Chem 277:46974-46979. (Pubitemid 36159202)
29. Kalo MS, Yu H-H, Pasquale EB (2001) In vivo tyrosine phosphorylation sites of activated Ephrin-B1 and EphB2 from neural tissue. J Biol Chem 276:38940-38948.
30. Lawrenson ID, Wimmer-Kleikamp SH, Lock P, Schoenwaelder SM, Down M, Boyd AW, Alewood PF, Lackmann M (2002) Ephrin-A5 induces rounding, blebbing and de-adhesion of EphA3-expressing 293T and melanoma cells by CrkII and Rho-mediated signalling. J Cell Sci 115:1059-1072. (Pubitemid 34257389)
31. Zhuang G, Hunter S, Hwang Y, Chen J (2007) Regulation of EphA2 receptor endocytosis by SHIP2 lipid phosphatase via phosphatidylinositol 3-kinase-dependent Rac1 activation. J Biol Chem 282:2683-2694. (Pubitemid 47076792)
32. Hunter SG, Zhuang G, Brantley-Sieders D, Swat W, Cowan CW, Chen J (2006) Essential role of Vav family guanine nucleotide exchange factors in EphA receptor-mediated angiogenesis. Mol Cell Biol 26:4830-4842. (Pubitemid 43955801)