Quantitative, noninvasive imaging of radiation-induced DNA double-strand breaks in vivo

Cancer Research

Volumn 71, Issue 12, 2011, Pages 4130-4137

  Li, Wenrong ^a,c   Li, Fang ^a   Huang, Qian ^a,d   Shen, Jingping ^a   Wolf, Frank ^a   He, Yujun ^a   Liu, Xinjian ^a   Hu, Y Angela ^a   Bedford, Joel S ^b   Li, Chuan Yuan ^a  

^a UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

^b Department of Environmental and Radiological Health Sciences   (United States)

^c Xinjiang Academy of Animal Science   (China)

^d SHANGHAI FIRST PEOPLE S HOSPITAL   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; HISTONE H2AX; IRON; LUCIFERASE; MEDIATOR OF DNA DAMAGE CHECKPOINT 1 PROTEIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DOUBLE STRANDED DNA BREAK; IN VITRO STUDY; IN VIVO STUDY; IONIZING RADIATION; MAMMAL CELL; MOLECULAR IMAGING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; QUANTITATIVE STUDY; RADIATION INJURY REPAIR; REPORTER GENE; TUMOR XENOGRAFT; X RAY;

CELL LINE, TUMOR; DNA BREAKS, DOUBLE-STRANDED; HEAVY IONS; HISTONES; HUMANS; LUCIFERASES; NEOPLASMS, EXPERIMENTAL; NUCLEAR PROTEINS; PHOSPHORYLATION; TRANS-ACTIVATORS; X-RAYS;

EID: 79958819362     PISSN: 00085472     EISSN: 15387445     Source Type: Journal    
DOI: 10.1158/0008-5472.CAN-10-2540     Document Type: Article

References (36)

1. Obe G, Johannes C, Schulte-Frohlinde D. DNA double-strand breaks induced by sparsely ionizing radiation and endonucleases as critical lesions for cell death, chromosomal aberrations, mutations and oncogenic transformation. Mutagenesis 1992;7:3-12.
2. Natarajan AT, Obe G. Molecular mechanisms involved in the production of chromosomal aberrations. I. Utilization of Neurospora endonuclease for the study of aberration production in G2 stage of the cell cycle. Mutat Res 1978;52:137-49.
3. Natarajan AT, Obe G, van Zeeland AA, Palitti F, Meijers M, Verdegaal-Immerzeel EA. Molecular mechanisms involved in the production of chromosomal aberrations. II. Utilization of Neurospora endonuclease for the study of aberration production by X-rays in G1 and G2 stages of the cell cycle. Mutat Res 1980;69:293-305.
4. O'Driscoll M, Jeggo PA. The role of double-strand break repair-insights from human genetics. Nat Rev Genet 2006;7:45-54. (Pubitemid 41828947)
5. Negrini S, Gorgoulis VG, Halazonetis TD. Genomic instability-an evolving hallmark of cancer. Nat Rev Mol Cell Biol 2010;11:220-8.
6. Banin S, Moyal L, Shieh S, Taya Y, Anderson CW, Chessa L, et al. Enhanced phosphorylation of p53 by ATM in response to DNA damage. Science 1998;281:1674-7.
7. Canman CE, Lim DS, Cimprich KA, Taya Y, Tamai K, Sakaguchi K, et al. Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Science 1998;281:1677-9. (Pubitemid 28435584)
8. Cucinotta FA, Pluth JM, Anderson JA, Harper JV, O'Neill P. Biochemical kinetics model of DSB repair and induction of gamma-H2AX foci by non-homologous end joining. Radiat Res 2008;169:214-22. (Pubitemid 351295079)
9. Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM. DNA doublestranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 1998;273:5858-68. (Pubitemid 28124064)
10. Rogakou EP, Boon C, Redon C, Bonner WM. Megabase chromatin domains involved in DNA double-strand breaks in vivo. J Cell Biol 1999;146:905-16.
11. Nakada S, Chen GI, Gingras AC, Durocher D. PP4 is a gamma H2AX phosphatase required for recovery from the DNA damage checkpoint. EMBO Rep 2008;9:1019-26.
12. Siino JS, Nazarov IB, Svetlova MP, Solovjeva LV, Adamson RH, Zalenskaya IA, et al. Photobleaching of GFP-labeled H2AX in chromatin: H2AX has low diffusional mobility in the nucleus. Biochem Biophys Res Commun 2002;297:1318-23. (Pubitemid 35216391)
13. Kruhlak MJ, Celeste A, Dellaire G, Fernandez-Capetillo O, Muller WG, McNally JG, et al. Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks. J Cell Biol 2006;172:823-34.
14. Stewart GS, Wang B, Bignell CR, Taylor AM, Elledge SJ. MDC1 is a mediator of the mammalian DNA damage checkpoint. Nature 2003;421:961-6. (Pubitemid 36288029)
15. Goldberg M, Stucki M, Falck J, D'Amours D, Rahman D, Pappin D, et al. MDC1 is required for the intra-S-phase DNA damage checkpoint. Nature 2003;421:952-6. (Pubitemid 36288027)
16. Li W, Li F, Huang Q, Frederick B, Bao S, Li CY. Noninvasive imaging and quantification of epidermal growth factor receptor kinase activation in vivo. Cancer Res 2008;68:4990-7.
17. Stucki M, Clapperton JA, Mohammad D, Yaffe MB, Smerdon SJ, Jackson SP. MDC1 directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks. Cell 2005;123:1213-26. (Pubitemid 41821780)
18. Chang WP, Little JB. Persistently elevated frequency of spontaneous mutations in progeny of CHO clones surviving X-irradiation: association with delayed reproductive death phenotype. Mutat Res 1992;270:191-9.
19. Ponnaiya B, Cornforth MN, Ullrich RL. Induction of chromosomal instability in human mammary cells by neutrons and gamma rays. Radiat Res 1997;147:288-94. (Pubitemid 27114384)
20. Little JB. Induction of genetic instability by ionizing radiation. C R Acad Sci III 1999;322:127-34.
21. Li C-Y, Little J, Hu K, Zhang W, Zhang L, Dewhirst M, et al. Persistent genetic instability in cancer cells induced by non-DNA damaging stress exposures. Cancer Research 2001;61:428-32. (Pubitemid 32128591)
22. Williams ES, Klingler R, Ponnaiya B, Hardt T, Schrock E, Lees-Miller SP, et al. Telomere dysfunction and DNA-PKcs deficiency: characterization and consequence. Cancer Res 2009;69:2100-7.
23. Klages N, Zufferey R, Trono D. A stable system for the high-titer production of multiply attenuated lentiviral vectors. Mol Ther 2000;2:170-6.
24. Ausubel F, Brent R, Kingston P, Moore D, Seidman J, Smith J, et al., editors. Current protocols in molecular biology. 2nd ed. New York: John Wiley & Sons; 1994.
25. Nicholson DW, Ali A, Thornberry NA, Vaillancourt JP, Ding CK, Gallant M, et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 1995;376:37-43.
26. Lazebnik YA, Kaufmann SH, Desnoyers S, Poirier GG, Earnshaw WC. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 1994;371:346-7. (Pubitemid 24300618)
27. Di Leonardo A, Linke SP, Clarkin K, Wahl GM. DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts. Genes Dev 1994;8:2540-51. (Pubitemid 24347245)
28. Mukherjee B, Camacho CV, Tomimatsu N, Miller J, Burma S. Modulation of the DNA-damage response to HZE particles by shielding. DNA Repair (Amst) 2008;7:1717-30.
29. Asaithamby A, Uematsu N, Chatterjee A, Story MD, Burma S, Chen DJ. Repair of HZE-particle-induced DNA double-strand breaks in normal human fibroblasts. Radiat Res 2008;169:437-46. (Pubitemid 351620042)
30. Desai N, Davis E, O'Neill P, Durante M, Cucinotta FA, Wu H. Immunofluorescence detection of clustered gamma-H2AX foci induced by HZE-particle radiation. Radiat Res 2005;164:518-22. (Pubitemid 41368929)
31. Roig AI, Hight SK, Minna JD, Shay JW, Rusek A, Story MD. DNA damage intensity in fibroblasts in a 3-dimensional collagen matrix correlates with the Bragg curve energy distribution of a high LET particle. Int J Radiat Biol 2010;86:194-204.
32. Anderson JA, Harper JV, Cucinotta FA, O'Neill P. Participation of DNA-PKcs in DSB repair after exposure to high- and low-LET radiation. Radiat Res 2010;174:195-205.
33. Bernier J, Hall EJ, Giaccia A. Radiation oncology: a century of achievements. Nat Rev Cancer 2004;4:737-47.
34. Hall EJ, Giaccia AJ. Radiobiology for the radiologist. 6th ed. Philadelphia: Lippincott Williams & Wilkins; 2006.
35. Li F, Huang Q, Chen J, Peng Y, Roop DR, Bedford JS, et al. Apoptotic cells activate the "phoenix rising" pathway to promote wound healing and tissue regeneration. Sci Signal 2010;3:ra13.
36. Li F, He Z, Shen J, Huang Q, Li W, Liu X, et al. Apoptotic caspases regulate induction of iPSCs from human fibroblasts. Cell Stem Cell 2010;7:508-20.