Immunofluorescence detection of clustered γ-H2AX foci induced by HZE-particle radiation

Radiation Research

Volumn 164, Issue 4 II, 2005, Pages 518-522

  Desai, N ^a   Davis, E ^b   O'Neill, P ^b   Durante, M ^c   Cucinotta, F A ^d   Wu, H ^d  

^a WYLE LABORATORIES   (United States)

^b MEDICAL RESEARCH COUNCIL   (United Kingdom)

^c UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^d NASA JOHNSON SPACE CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; HISTONE H2A; HISTONE H2AX; UNCLASSIFIED DRUG;

CONFERENCE PAPER; CONTROLLED STUDY; DNA DAMAGE; DNA STRAND BREAKAGE; FIBROBLAST; HUMAN; HUMAN CELL; HZE PARTICLE RADIATION; IMMUNOFLUORESCENCE; IRRADIATION; PARTICLE RADIATION; PHOSPHORYLATION; PREPUCE; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; RADIATION INJURY;

NASA CENTER JSC; NASA DISCIPLINE RADIATION HEALTH;

CELLS, CULTURED; COSMIC RADIATION; DNA DAMAGE; DNA REPAIR; FLUORESCENT ANTIBODY TECHNIQUE; HISTONES; HUMANS; PHOSPHORYLATION;

EID: 25444502873     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.1667/RR3431.1     Document Type: Conference Paper

References (17)

1. F. A. Cucinotta, H. Wu, M. R. Shavers and K. George, Radiation dosimetry and biophysical models of space radiation effects. Gravit. Space Biol. Bull. 16, 11-18 (2003).
2. D. T. Goodhead, The initial physical damage produced by ionizing radiations. Int. J. Radiat. Biol. 56, 623-634 (1989).
3. F. A. Cucinotta, H. Nikjoo and D. T. Goodhead, Radial distribution of energy imparted in nanometer volumes from HZE particles. Radiat. Res. 153, 459-468 (2000).
4. T. J. Jenner, C. M. Delara, P. O'Neill and D. L. Stevens, Induction and rejoining of DNA double-strand breaks following γ- and α-irradiation. Int. J. Radiat. Biol. 64, 265-273 (1993).
5. K. M. Prise, M. Pinto, H. C. Newman and B. D. Michael, A review of studies of ionizing radiation-induced double-strand break clustering. Radiat. Res. 156, 572-576 (2001).
6. C. Baumstark-Khan, J. Heilmann and H. Rink, Induction and repair of DNA strand breaks in bovine lens epithelial cells after high LET irradiation. Adv. Space Res. 31, 1583-1591 (2003).
7. H. Hoglund and B. Stenerlow, Induction and rejoining of DNA double-strand breaks in normal human skin fibroblasts alter exposure to radiation of different linear energy transfer: Possible roles of track structure and chromatin organization. Radiat. Res. 155, 818-825 (2001).
8. B. Jakob, M. Scholz and G. Taucher-Scholz, Characterization of CDKN1A (p21) binding to sites of heavy-ion-induced damage: Colocalization with proteins involved in DNA repair. Int. J. Radiat. Biol. 78, 75-88 (2002).
9. B. Jakob, M. Scholz and G. Taucher-Scholz, Biological imaging of heavy charged-particle tracks. Radiat. Res. 159, 676-684 (2003).
10. D. R. Pilch, O. A. Sedelnikova, C. Redon, A. Celeste, A. Nussenzweig and W. M. Bonner, Characteristics of gamma-H2AX foci at DNA double-strand breaks sites. Biochem. Cell Biol. 81, 123-129 (2003).
11. E. P. Rogakou, C. Boon, C. Redon and W. M. Bonner, Megabase chromatin domains involved in DNA double-strand breaks in vivo. J. Cell Biol. 146, 905-916 (1999).
12. E. P. Rogakou, D. R. Pilch, A. H. Orr, V. S. Ivanova and W. M. Bonner, DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J. Biol. Chem. 273, 5858-5868 (1998).
13. K. Rothkamm and M. Lobrich, Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses. Proc. Natl. Acad. Sci. USA 100, 5057-5057 (2003).
14. S. Goto, M. Watanabe and F. Yatagi, Delayed cell cycle progression in human lymphoblastoid cells after exposure to high-LET radiation correlates with extremely localized DNA damage. Radiat. Res. 158, 678-686 (2002).
15. P. L. Olive and J. P. Banath, Phosphorylation of historic H2AX as a measure of radiosensitivity. Int. J. Radiat. Oncol. Biol. Phys. 58, 331-335 (2004).
16. J. A. Aten, J. Stap, P. M. Krawezyk, C. H. van Oven, R. A. Hoebe, J. Essers and R. Kanaar, Dynamics of DNA double-strand breaks revealed by clustering of damaged chromosome domains. Science 303, 92-95 (2004).
17. 1-phase cells. Radiat. Res. 159, 759-767 (2003).