Comparison of different PCR tests for detecting Shiga toxin-producing Escherichia coli O157 and development of an ELISA-PCR assay for specific identification of the bacteria

Journal of Microbiological Methods

Volumn 55, Issue 2, 2003, Pages 383-392

  Fach, Patrick ^a   Perelle, Sylvie ^a   Grout, Joël ^a   Dilasser, Françoise ^a  

^a Healthy Pigs Production and Experimentation Section   (France)

Author keywords

ELISA PCR; Enterohemorrhagic Escherichia coli; O157

Indexed keywords

SHIGA TOXIN;

ACCURACY; AMPLICON; ARTICLE; BACTERIUM IDENTIFICATION; DNA HYBRIDIZATION; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI O157; GENE AMPLIFICATION; GENE FREQUENCY; MOLECULAR PROBE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCREENING;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0141643388     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(03)00172-6     Document Type: Article

References (32)

1. Aleksic S., Karch H., Bockemuhl J. A biotyping scheme for Shiga-like (Vero) toxin-producing Escherichia coli O157 and a list of serological cross-reactions between O157 and other gram-negative bacteria. Zbl. Bakt. 276:1992;221-230.
2. Bettelheim K.A., Evangelidis H., Pearce J.L., Sowers E., Strockbine N.A. Isolation of a Citrobacter freundii strain which carries the Escherichia coli O157 antigen. J. Clin. Microbiol. 31:1993;760-761.
3. Borczyk A.A., Lior H., Ciebin B. False positive identifications of Escherichia coli O157 in foods. Int. J. Food. Microbiol. 4:1987;347-349.
4. Brunder W., Schmidt H., Karch H. KatP, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli O157:H7. Microbiology. 142:1996;3305-3315.
5. Cebula T.A., Payne W.L., Feng P. Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. J. Clin. Microbiol. 33:1995;248-250. (published erratum appears in J. Clin. Microbiol. 33, 1048).
6. Chart H., Okubadejo O.A., Rowe B. The serological relationship between Escherichia coli O157 and Yersinia enterocolitica O9 using sera from patients with brucellosis. Epidemiol. Infect. 108:1992;77-85.
7. Deng M.Y., Fratamico P.M. A multiplex PCR for rapid identification of Shiga-like toxin-producing Escherichia coli O157:H7 isolated from foods. J. Food. Prot. 59:1996;570-576.
8. Desmarchelier P.M., Bilge S.S., Fegan N., Mills L., Vary J.C. Jr., Tarr P.I. A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide. J. Clin. Microbiol. 36:1998;1801-1804.
9. Fach P., Perelle S., Dilasser F., Grout J. Comparison between a PCR-ELISA test and the Vero cell assay for detecting Shiga toxin-producing Escherichia coli in dairy products and characterization of virulence traits of the isolated strains. J. Appl. Microbiol. 90:2001;809-818.
10. Fratamico P.M., Sackitey S.K., Wiedmann M., Deng M.Y. Detection of Escherichia coli O157:H7 by multiplex PCR. J. Clin. Microbiol. 33:1995;2188-2191.
11. Gannon V.P.J., D'Souza S., Graham T., King R.K., Rhan K., Read S. Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains. J. Clin. Microbiol. 35:1997;656-662.
12. Gunzer F., Bohm H., Russmann H., Bitzan M., Aleksic S., Karch H. Molecular detection of sorbitol-fermenting Escherichia coli O157 in patients with hemolytic-uremic syndrome. J. Clin. Microbiol. 30:1992;1807-1810.
13. Konowalchuk J., Speirs J.I., Stavric S. Vero response to a cytotoxin of Escherichia coli. Infect. Immun. 18:1977;775-779.
14. Louie M., De Azavedo J., Clarke R., Borczyk A., Lior H., Richter M., Brunton J. Sequence heterogeneity of the eae gene and detection of verotoxin-producing Escherichia coli using serotype-specific primers. Epidemiol. Infect. 112:1994;449-461.
15. Maurer J.J., Schmidt D., Petrosko P., Sanchez S., Bolton L., Lee M.D. Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR. Appl. Environ. Microbiol. 65:1999;2954-2960.
16. Meng J., Zhao S., Doyle M.P., Mitchell S.E., Kresovich S. Polymerase chain reaction for detecting Escherichia coli O157:H7. Int. J. Food. Microbiol. 32:1996;103-113.
17. Nagano I., Kunishima M., Itoh Y., Wu Z., Takahashi Y. Detection of verotoxin-producing Escherichia coli O157:H7 by multiplex polymerase chain reaction. Microbiol. Immunol. 42:1998;371-376.
18. Nataro J.P., Kaper J.B. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11:1998;142-201. (published erratum appears in Clin. Microbiol. Rev. 11, 403).
19. Oberst R.D., Hays M.P., Bohra L.K., Phebus R.K., Yamashiro C.T., Paszko-Kolva C., Flood S.J., Sargeant J.M., Gillespie J.R. PCR-based DNA amplification and presumptive detection of Escherichia coli O157:H7 with an internal fluorogenic probe and the 5′ nuclease (TaqMan) assay. Appl. Environ. Microbiol. 64:1998;3389-3396.
20. O157. J. Clin. Microbiol. 36:1998;598-602.
21. 157). J. Appl. Microbiol. 93:2002;250-260.
22. Perera L.P., Marques L.R., O'Brien A.D. Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay. J. Clin. Microbiol. 26:1988;2127-2131.
23. Pollard D.R., Johnson W.M., Lior H., Tyler S.D., Rozee K.R. Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction. J. Clin. Microbiol. 28:1990;540-545.
24. Pollard D.R., Johnson W.M., Lior H., Tyler S.D., Rozee K.R. Differentiation of Shiga toxin and Vero cytotoxin type 1 genes by polymerase chain reaction. J. Infect. Dis. 162:1990;1195-1198.
25. Sandhu K.S., Clarke R.C., McFadden K., Brouwer A., Louie M., Wilson J., Lior H., Gyles C.L. Prevalence of the eaeA gene in verotoxigenic Escherichia coli strains from dairy cattle in Southwest Ontario. Epidemiol. Infect. 116:1996;1-7.
26. Schmidt H., Beutin L., Karch H. Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933. Infect. Immun. 63:1995;1055-1061.
27. Sharma V.K. Detection and quantitation of enterohemorrhagic Escherichia coli O157, O111, and O26 in beef and bovine feces by real-time polymerase chain reaction. J. Food Prot. 65:2002;1371-1380.
28. Sharma V.K., Dean-Nystrom E.A. Detection of enterohemorrhagic Escherichia coli O157:H7 by using a multiplex real-time PCR assay for genes encoding intimin and Shiga toxins. Vet. Microbiol. 93:2003;247-260.
29. 1. Curr. Microbiol. 25:1992;215-217.
30. Strockbine N.A., Marques L.R., Holmes R.K., O'Brien A.D. Characterization of monoclonal antibodies against Shiga-like toxin from Escherichia coli. Infect. Immun. 50:1985;695-700.
31. Strockbine N.A., Marques L.R.M., Newland J.W., Smith H.W., Holmes R.K., O'Brien A.D. Two toxin-converting phages from Escherichia coli O157:H7 strain 933 encode antigenically distinct toxins with similar biologic activities. Infect. Immun. 53:1986;135-140.
32. Thompson J.S., Hodge D.S., Borczyk A.A. Rapid biochemical test to identify verocytotoxin-positive strains of Escherichia coli serotype O157. J. Clin. Microbiol. 28:1990;2165-2168.