A filter microplate assay for quantitative analysis of DNA binding proteins using fluorescent DNA

Analytical Biochemistry

Volumn 415, Issue 2, 2011, Pages 168-174

  Yang, William C ^a   Swartz, James R ^a  

^a Stanford University   (United States)

Author keywords

Cell free protein synthesis; DNA binding; Transcription factor

Indexed keywords

BINDING ENERGY; BIOCHEMISTRY; BIOSYNTHESIS; DNA; FLUORESCENCE; PROTEINS; RADIATION; RADIOACTIVITY; TRANSCRIPTION; TRANSCRIPTION FACTORS;

APPROXIMATE AGREEMENT; CELL FREE PROTEIN SYNTHESIS; COMPETITIVE BINDING; DNA BINDING; DNA BINDING AFFINITY; DNA-BINDING PROTEIN; FLUORESCENCE SIGNALS; NITRILOTRIACETIC ACID;

DNA SEQUENCES;

AGAROSE; DNA BINDING PROTEIN; FLUORESCENT DYE; IMMOBILIZED PROTEIN; NITRILOTRIACETATE NICKEL; POLYHISTIDINE TAG; RECOMBINANT PROTEIN; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR SOX2;

ARTICLE; BINDING AFFINITY; BINDING ASSAY; BINDING COMPETITION; CONSENSUS SEQUENCE; CONTROLLED STUDY; DNA PROBE; HUMAN; ISOTOPE LABELING; MICROTITER PLATE ASSAY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DNA BINDING; QUANTITATIVE ANALYSIS;

EID: 79958286932     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.03.027     Document Type: Article

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