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Volumn 415, Issue 2, 2011, Pages 151-157

Pitfalls of reverse transcription quantitative polymerase chain reaction standardization: Volume-related inhibitors of reverse transcription

Author keywords

Inhibitor; MIQE guidelines; mRNA quantification; Reverse transcription; RT qPCR; Standardization

Indexed keywords

RELIABILITY; RNA; STANDARDIZATION;

EID: 79958283994     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.04.008     Document Type: Article
Times cited : (12)

References (24)
  • 1
    • 77649233414 scopus 로고    scopus 로고
    • Why the need for qPCR publication guidelines? the case for MIQE
    • S.A. Bustin Why the need for qPCR publication guidelines? The case for MIQE Methods 50 2010 217 226
    • (2010) Methods , vol.50 , pp. 217-226
    • Bustin, S.A.1
  • 3
    • 22044458051 scopus 로고    scopus 로고
    • Real-time, fluorescence-based quantitative PCR: A snapshot of current procedures and preferences
    • DOI 10.1586/14737159.5.4.493
    • S.A. Bustin Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences Expert Rev. Mol. Diagn. 5 2005 493 498 (Pubitemid 40966418)
    • (2005) Expert Review of Molecular Diagnostics , vol.5 , Issue.4 , pp. 493-498
    • Bustin, S.A.1
  • 4
    • 1542328972 scopus 로고    scopus 로고
    • Properties of the Reverse Transcription Reaction in mRNA Quantification
    • DOI 10.1373/clinchem.2003.026161
    • A. Stahlberg, J. Hakansson, X. Xian, H. Semb, and M. Kubista Properties of the reverse transcription reaction in mRNA quantification Clin. Chem. 50 2004 509 515 (Pubitemid 38297299)
    • (2004) Clinical Chemistry , vol.50 , Issue.3 , pp. 509-515
    • Stahlberg, A.1    Hakansson, J.2    Xian, X.3    Semb, H.4    Kubista, M.5
  • 5
    • 0036222053 scopus 로고    scopus 로고
    • Low efficiency of the Moloney murine leukemia virus reverse transcriptase during reverse transcription of rare t(8;21) fusion gene transcripts
    • J. Curry, C. McHale, and M.T. Smith Low efficiency of the Moloney murine leukemia virus reverse transcriptase during reverse transcription of rare t(8;21) fusion gene transcripts BioTechniques 32 2002 754 772
    • (2002) BioTechniques , vol.32 , pp. 754-772
    • Curry, J.1    McHale, C.2    Smith, M.T.3
  • 7
    • 37549052481 scopus 로고    scopus 로고
    • Detection limits of several commercial reverse transcriptase enzymes: Impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR
    • J.P. Levesque-Sergerie, M. Duquette, C. Thibault, L. Delbecchi, and N. Bissonnette Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR BMC Mol. Biol. 8 2007 93
    • (2007) BMC Mol. Biol. , vol.8 , pp. 93
    • Levesque-Sergerie, J.P.1    Duquette, M.2    Thibault, C.3    Delbecchi, L.4    Bissonnette, N.5
  • 8
    • 0030748867 scopus 로고    scopus 로고
    • Inhibition and facilitation of nucleic acid amplification
    • I.G. Wilson Inhibition and facilitation of nucleic acid amplification Appl. Environ. Microbiol. 63 1997 3741 3751 (Pubitemid 27411322)
    • (1997) Applied and Environmental Microbiology , vol.63 , Issue.10 , pp. 3741-3751
    • Wilson, I.G.1
  • 9
    • 2642663514 scopus 로고    scopus 로고
    • Myoglobin as a polymerase chain reaction (pcr) inhibitor: A limitation for pcr from skeletal muscle tissue avoided by the use of thermus thermophilus polymerase
    • DOI 10.1002/(SICI)1097-4598(1998 08)21:8<1064::AID-MUS11>3.0.CO;2-U
    • L. Belec, J. Authier, M.C. Eliezer-Vanerot, C. Piedouillet, A.S. Mohamed, and R.K. Gherardi Myoglobin as a polymerase chain reaction (PCR) inhibitor: a limitation for PCR from skeletal muscle tissue avoided by the use of Thermus thermophilus polymerase Muscle Nerve 21 1998 1064 1067 (Pubitemid 28287997)
    • (1998) Muscle and Nerve , vol.21 , Issue.8 , pp. 1064-1067
    • Belec, L.1    Authier, J.2    Eliezer-Vanerot, M.-C.3    Piedouillet, C.4    Mohamed, A.S.5    Gherardi, R.K.6
  • 10
    • 33748741378 scopus 로고    scopus 로고
    • Repeat silica extraction: A simple technique for the removal of PCR inhibitors from DNA extracts
    • DOI 10.1016/j.jas.2006.02.015, PII S0305440306000550
    • B.M. Kemp, C. Monroe, and D.G. Smith Repeat silica extraction: a simple technique for the removal of PCR inhibitors from DNA extracts J. Archaeol. Sci. 33 2006 1680 1689 (Pubitemid 44400536)
    • (2006) Journal of Archaeological Science , vol.33 , Issue.12 , pp. 1680-1689
    • Kemp, B.M.1    Monroe, C.2    Smith, D.G.3
  • 12
    • 0026678203 scopus 로고
    • Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions
    • DOI 10.1016/0168-1605(92)90017-W
    • L. Rossen, P. Norskov, K. Holmstrom, and O.F. Rasmussen Inhibition of PCR by components of food samples, microbial diagnostic assays, and DNA-extraction solutions Intl. J. Food Microbiol. 17 1992 37 45 (Pubitemid 23000398)
    • (1992) International Journal of Food Microbiology , vol.17 , Issue.1 , pp. 37-45
    • Rossen, L.1    Norskov, P.2    Holmstrom, K.3    Rasmussen, O.F.4
  • 15
    • 33645293441 scopus 로고    scopus 로고
    • SPUD: A quantitative PCR assay for the detection of inhibitors in nucleic acid preparations
    • T. Nolan, R.E. Hands, W. Ogunkolade, and S.A. Bustin SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations Anal. Biochem. 351 2006 308 310
    • (2006) Anal. Biochem. , vol.351 , pp. 308-310
    • Nolan, T.1    Hands, R.E.2    Ogunkolade, W.3    Bustin, S.A.4
  • 16
    • 33644975004 scopus 로고    scopus 로고
    • RNA integrity and the effect on the real-time qRT-PCR performance
    • S. Fleige, and M.W. Pfaffl RNA integrity and the effect on the real-time qRT-PCR performance Mol. Aspects Med. 27 2006 126 139
    • (2006) Mol. Aspects Med. , vol.27 , pp. 126-139
    • Fleige, S.1    Pfaffl, M.W.2
  • 17
    • 33847643776 scopus 로고    scopus 로고
    • Quantification of mRNA using real-time RT-PCR
    • T. Nolan, R.E. Hands, and S.A. Bustin Quantification of mRNA using real-time RT-PCR Nat. Protoc. 1 2006 1559 1582
    • (2006) Nat. Protoc. , vol.1 , pp. 1559-1582
    • Nolan, T.1    Hands, R.E.2    Bustin, S.A.3
  • 18
    • 0042121256 scopus 로고    scopus 로고
    • Mfold web server for nucleic acid folding and hybridization prediction
    • DOI 10.1093/nar/gkg595
    • M. Zuker Mfold web server for nucleic acid folding and hybridization prediction Nucleic Acids Res. 31 2003 3406 3415 (Pubitemid 37442169)
    • (2003) Nucleic Acids Research , vol.31 , Issue.13 , pp. 3406-3415
    • Zuker, M.1
  • 20
    • 4344564046 scopus 로고    scopus 로고
    • Comparison of reverse transcriptases in gene expression analysis
    • DOI 10.1373/clinchem.2004.035469
    • A. Stahlberg, M. Kubista, and M. Pfaffl Comparison of reverse transcriptases in gene expression analysis Clin. Chem. 50 2004 1678 1680 (Pubitemid 39121243)
    • (2004) Clinical Chemistry , vol.50 , Issue.9 , pp. 1678-1680
    • Stahlberg, A.1    Kubista, M.2    Pfaffl, M.3
  • 21
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • RESEARCH0034
    • J. Vandesompele, K. De Preter, F. Pattyn, B. Poppe, N. Van Roy, A. De Paepe, F. Speleman, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes, Genome Biol. 3 (2002) RESEARCH0034.
    • (2002) Genome Biol. , vol.3
    • Vandesompele, J.1    De Preter, K.2    Pattyn, F.3    Poppe, B.4    Van Roy, N.5    De Paepe, A.6    Speleman, F.7
  • 22
    • 0028828597 scopus 로고
    • Secondary structure in the 3′ UTR of EGF and the choice of reverse transcriptases affect the detection of message diversity by RT-PCR
    • E.M. Brooks, L.G. Sheflin, and S.W. Spaulding Secondary structure in the 3′ UTR of EGF and the choice of reverse transcriptases affect the detection of message diversity by RT-PCR BioTechniques 19 1995 806 812 814-815
    • (1995) BioTechniques , vol.19 , pp. 806-812
    • Brooks, E.M.1    Sheflin, L.G.2    Spaulding, S.W.3
  • 23
    • 0030780417 scopus 로고    scopus 로고
    • Intrinsic secondary structure of human TNFR-I mRNA influences the determination of gene expression by RT-PCR
    • K.W. Kuo, M.F. Leung, and W.C. Leung Intrinsic secondary structure of human TNFR-I mRNA influences the determination of gene expression by RT-PCR Mol. Cell. Biochem. 177 1997 1 6 (Pubitemid 27522351)
    • (1997) Molecular and Cellular Biochemistry , vol.177 , Issue.1-2 , pp. 1-6
    • Kuo, K.-W.1    Leung, M.F.2    Leung, W.-C.3
  • 24
    • 66549129231 scopus 로고    scopus 로고
    • Reliability of real-time reverse-transcription PCR in clinical diagnostics: Gold standard or substandard?
    • J. Murphy, and S.A. Bustin Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard? Expert Rev. Mol. Diagn. 9 2009 187 197
    • (2009) Expert Rev. Mol. Diagn. , vol.9 , pp. 187-197
    • Murphy, J.1    Bustin, S.A.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.