Production and purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors

Gene Therapy

Volumn 18, Issue 6, 2011, Pages 531-538

  Lesch, H P ^a,b   Laitinen, A ^a   Peixoto, C ^c   Vicente, T ^c,d   Makkonen, K E ^a   Laitinen, L ^b   Pikkarainen, J T ^a,b   Samaranayake, H ^a,b   Alves, P M ^c   Carrondo, M J T ^c   Yla Herttuala S ^a,e   Airenne, K J ^a  

^a UNIVERSITY OF EASTERN FINLAND   (Finland)

^b Finland Ark Therapeutics Oy   (Finland)

^c IBET   (Portugal)

^d INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^e KUOPIO UNIVERSITY HOSPITAL   (Finland)

Author keywords

baculovirus; lentivirus; production; purification; scalability; suspension

Indexed keywords

BACULOVIRUS VECTOR; CALCIUM PHOSPHATE; DIETHYLAMINOETHYL CELLULOSE; GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR; POLYETHYLENEIMINE;

ADHERENT CELL; ARTICLE; CELL DENSITY; CELL GROWTH; CELL SUSPENSION; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DRUG SAFETY; EMBRYO; FEMALE; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; GENE VECTOR; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PURIFICATION; RAT; SCALE UP; SUSPENSION CELL CULTURE; VIRAL GENE DELIVERY SYSTEM; VIRUS PURIFICATION;

ANIMALS; BACULOVIRIDAE; CELL CULTURE TECHNIQUES; CELL LINE; ETHANOLAMINES; GENETIC VECTORS; LENTIVIRUS; ORGANISMS, GENETICALLY MODIFIED; RATS; TRANSDUCTION, GENETIC; TRANSFECTION;

LENTIVIRUS;

EID: 79958259186     PISSN: 09697128     EISSN: 14765462     Source Type: Journal    
DOI: 10.1038/gt.2010.162     Document Type: Article

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