BacMam system for high-level expression of recombinant soluble and membrane glycoproteins for structural studies

Protein Expression and Purification

Volumn 62, Issue 2, 2008, Pages 160-170

  Dukkipati, Abhiram ^a   Park, Hyun Ho ^a   Waghray, Deepa ^a   Fischer, Suzanne ^a   Garcia, K Christopher ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

293 cells; BacMam; Chemokine; Crystallization; Fractalkine; Frizzled; GPCR; Parathyroid hormone receptor; Purification; US28

Indexed keywords

CHEMOKINE RECEPTOR; FRACTALKINE; FRIZZLED PROTEIN; FZD4 PROTEIN, MOUSE; G PROTEIN COUPLED RECEPTOR; MEMBRANE PROTEIN; PARATHYROID HORMONE RECEPTOR 1; RECOMBINANT PROTEIN; US28 RECEPTOR, CYTOMEGALOVIRUS; VIRUS PROTEIN;

ANIMAL; ARTICLE; BACULOVIRUS; CELL LINE; CHEMISTRY; GENE VECTOR; GENETIC TRANSDUCTION; GENETIC TRANSFECTION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; PROTEIN TERTIARY STRUCTURE; SOLUBILITY; X RAY CRYSTALLOGRAPHY;

ANIMALS; BACULOVIRIDAE; BASE SEQUENCE; CELL LINE; CHEMOKINE CX3CL1; CRYSTALLOGRAPHY, X-RAY; FRIZZLED RECEPTORS; GENETIC VECTORS; HUMANS; MEMBRANE GLYCOPROTEINS; MICE; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; RECEPTOR, PARATHYROID HORMONE, TYPE 1; RECEPTORS, CHEMOKINE; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT PROTEINS; SOLUBILITY; TRANSDUCTION, GENETIC; TRANSFECTION; VIRAL PROTEINS;

MAMMALIA;

EID: 54049097435     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.08.004     Document Type: Article

References (30)

1. Boyce F.M., and Bucher N.L. Baculovirus-mediated gene transfer into mammalian cells. Proc. Natl. Acad. Sci. USA 93 (1996) 2348-2352
2. Condreay J.P., Witherspoon S.M., Clay W.C., and Kost T.A. Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. Proc. Natl. Acad. Sci. USA 96 (1999) 127-132
3. Hofmann C., Sandig V., Jennings G., Rudolph M., Schlag P., and Strauss M. Efficient gene transfer into human hepatocytes by baculovirus vectors. Proc. Natl. Acad. Sci. USA 92 (1995) 10099-10103
4. Shoji I., Aizaki H., Tani H., Ishii K., Chiba T., Saito I., Miyamura T., and Matsuura Y. Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors. J. Gen. Virol. 78 Pt 10 (1997) 2657-2664
5. Kost T.A., Condreay J.P., Ames R.S., Rees S., and Romanos M.A. Implementation of BacMam virus gene delivery technology in a drug discovery setting. Drug Discov. Today 12 (2007) 396-403
6. Scott M.J., Modha S.S., Rhodes A.D., Broadway N.M., Hardwicke P.I., Zhao H.J., Kennedy-Wilson K.M., Sweitzer S.M., and Martin S.L. Efficient expression of secreted proteases via recombinant BacMam virus. Protein Expr. Purif. 52 (2007) 104-116
7. Reeves P.J., Callewaert N., Contreras R., and Khorana H.G. Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line. Proc Natl. Acad. Sci. USA 99 (2002) 13419-13424
8. Muller N., Girard P., Hacker D.L., Jordan M., and Wurm F.M. Orbital shaker technology for the cultivation of mammalian cells in suspension. Biotechnol. Bioeng. 89 (2005) 400-406
9. Arakawa T., Philo J.S., Tsumoto K., Yumioka R., and Ejima D. Elution of antibodies from a Protein-A column by aqueous arginine solutions. Protein Expr. Purif. 36 (2004) 244-248
10. Buchman A.R., and Berg P. Comparison of intron-dependent and intron-independent gene expression. Mol. Cell. Biol. 8 (1988) 4395-4405
11. Huang M.T., and Gorman C.M. Intervening sequences increase efficiency of RNA 3′ processing and accumulation of cytoplasmic RNA. Nucleic Acids Res. 18 (1990) 937-947
12. Zufferey R., Donello J.E., Trono D., and Hope T.J. Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. J. Virol. 73 (1999) 2886-2892
13. Potts J.T. Parathyroid hormone: past and present. J. Endocrinol. 187 (2005) 311-325
14. Bisello A., Greenberg Z., Behar V., Rosenblatt M., Suva L.J., and Chorev M. Role of glycosylation in expression and function of the human parathyroid hormone/parathyroid hormone-related protein receptor. Biochemistry 35 (1996) 15890-15895
15. Grauschopf U., Lilie H., Honold K., Wozny M., Reusch D., Esswein A., Schafer W., Rucknagel K.P., and Rudolph R. The N-terminal fragment of human parathyroid hormone receptor 1 constitutes a hormone binding domain and reveals a distinct disulfide pattern. Biochemistry 39 (2000) 8878-8887
16. Monaghan P., Woznica I., Moza B., Sundberg E.J., and Rosenblatt M. Recombinant expression and purification of the N-terminal extracellular domain of the parathyroid hormone receptor. Protein Expr. Purif. 54 (2007) 87-93
17. Huang H.C., and Klein P.S. The frizzled family: receptors for multiple signal transduction pathways. Genome Biol. 5 (2004) 234
18. Robitaille J., MacDonald M.L., Kaykas A., Sheldahl L.C., Zeisler J., Dube M.P., Zhang L.H., Singaraja R.R., Guernsey D.L., Zheng B., Siebert L.F., Hoskin-Mott A., Trese M.T., Pimstone S.N., Shastry B.S., Moon R.T., Hayden M.R., Goldberg Y.P., and Samuels M.E. Mutant frizzled-4 disrupts retinal angiogenesis in familial exudative vitreoretinopathy. Nat. Genet. 32 (2002) 326-330
19. Gao J.L., and Murphy P.M. Human cytomegalovirus open reading frame US28 encodes a functional beta chemokine receptor. J. Biol. Chem. 269 (1994) 28539-28542
20. Kuhn D.E., Beall C.J., and Kolattukudy P.E. The cytomegalovirus US28 protein binds multiple CC chemokines with high affinity. Biochem. Biophys. Res. Commun. 211 (1995) 325-330
21. Kledal T.N., Rosenkilde M.M., and Schwartz T.W. Selective recognition of the membrane-bound CX3C chemokine, fractalkine, by the human cytomegalovirus-encoded broad-spectrum receptor US28. FEBS Lett. 441 (1998) 209-214
22. Bazan J.F., Bacon K.B., Hardiman G., Wang W., Soo K., Rossi D., Greaves D.R., Zlotnik A., and Schall T.J. A new class of membrane-bound chemokine with a CX3C motif. Nature 385 (1997) 640-644
23. Mizoue L.S., Sullivan S.K., King D.S., Kledal T.N., Schwartz T.W., Bacon K.B., and Handel T.M. Molecular determinants of receptor binding and signaling by the CX3C chemokine fractalkine. J. Biol. Chem. 276 (2001) 33906-33914
24. Pleskoff O., Treboute C., Brelot A., Heveker N., Seman M., and Alizon M. Identification of a chemokine receptor encoded by human cytomegalovirus as a cofactor for HIV-1 entry. Science 276 (1997) 1874-1878
25. Blackburn P.E., Simpson C.V., Nibbs R.J., O'Hara M., Booth R., Poulos J., Isaacs N.W., and Graham G.J. Purification and biochemical characterization of the D6 chemokine receptor. Biochem. J. 379 (2004) 263-272
26. Dukkipati A., Vaclavikova J., Waghray D., and Garcia K.C. In vitro reconstitution and preparative purification of complexes between the chemokine receptor CXCR4 and its ligands SDF-1alpha, gp120-CD4 and AMD3100. Protein Expr. Purif. 50 (2006) 203-214
27. 2-terminal domain of CC chemokine receptor 5 contribute to high affinity binding of chemokines. J. Exp. Med. 194 (2001) 1661-1673
28. Farzan M., Babcock G.J., Vasilieva N., Wright P.L., Kiprilov E., Mirzabekov T., and Choe H. The role of post-translational modifications of the CXCR4 amino terminus in stromal-derived factor 1 alpha association and HIV-1 entry. J. Biol. Chem. 277 (2002) 29484-29489
29. Mokros T., Rehm A., Droese J., Oppermann M., Lipp M., and Hopken U.E. Surface expression and endocytosis of the human cytomegalovirus-encoded chemokine receptor US28 is regulated by agonist-independent phosphorylation. J. Biol. Chem. 277 (2002) 45122-45128
30. Geisse S., Jordan M., and Wurm F.M. Large-scale transient expression of therapeutic proteins in mammalian cells. Methods Mol. Biol. 308 (2005) 87-98