Retroviral vector production under serum deprivation: The role of lipids

Biotechnology and Bioengineering

Volumn 104, Issue 6, 2009, Pages 1171-1181

  Rodrigues, A F ^a   Carmo, M ^a   Alves, P M ^a   Coroadinha, A S ^a  

^a INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

Author keywords

Cholesterol; Gene therapy; Lipids; Retroviral vectors; Serum deprivation; Virus production

Indexed keywords

CELL LINES; CLINICAL EFFICACY; CULTURE MEDIUM; GENE THERAPY; IN-CELL; QUALITY STANDARD; RETROVIRAL VECTORS; SERUM CONCENTRATION; SERUM DEPRIVATION; SERUM LIPID; SERUM-FREE CONDITIONS; TOTAL RECOVERY; VIRAL PRODUCTION; VIRAL RNA;

CELL CULTURE; CELLS; CHOLESTEROL; GALLIUM; GENETIC ENGINEERING; GROWTH KINETICS; LIPIDS; NUCLEIC ACIDS; PRODUCTIVITY; RNA; TELLURIUM COMPOUNDS; VECTORS;

BODY FLUIDS;

CHOLESTEROL; LIPID; RETROVIRUS VECTOR; VIRUS RNA;

ARTICLE; CELL CULTURE; CONTROLLED STUDY; CULTURE MEDIUM; HUMAN; HUMAN CELL; KINETICS; LIPID ANALYSIS; LIPID BLOOD LEVEL; NONHUMAN;

BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; CELL LINE; CULTURE MEDIA; GENETIC VECTORS; HUMANS; LIPIDS; RETROVIRIDAE; SERUM;

EID: 70449119110     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22499     Document Type: Article

References (33)

1. Alberts AW, Ferguson K, Hennessy S, Vagelos PR. 1974. Regulation of lipid synthesis in cultured animal cells. J Biol Chem 249(16):5241-5249.
2. Amaral AI, Coroadinha AS, Merten OW, Alves PM. 2008. Improving retroviral vectors production: Role of carbon sources in lipid biosynthesis. J Biotechnol 138(3-4):57-66.
3. Blesch A. 2004. Lentiviral and MLV based retroviral vectors for ex vivo and in vivo gene transfer. Methods 33(2):164-172. (Pubitemid 38569661)
4. Bligh EG, Dyer WJ. 1959. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37(8):911-917.
5. Briggs JA, Wilk T, Fuller SD. 2003. Do lipid rafts mediate virus assembly and pseudotyping? J Gen Virol 84(Pt 4):757-768.
6. Carmo M, Peixoto C, Coroadinha AS, Alves PM, Cruz PE, Carrondo MJT. 2004. Quantitation of MLV-based retroviral vectors using real-time RT-PCR. J Virol Methods 119(2):115-119. (Pubitemid 38670402)
7. Carmo M, Faria TQ, Falk H, Coroadinha AS, Teixeira M, Merten OW, Geny-Fiamma C, Alves PM, Danos O, Panet A, Carrondo MJT, Cruz PE. 2006. Relationship between retroviral vector membrane and vector stability. J Gen Virol 87(Pt 5):1349-1356.
8. Cham BE, Knowles BR. 1976. A solvent system for delipidation of plasma or serum without protein precipitation. J Lipid Res 17(2):176-181.
9. Chan LM, Coutelle C, Themis M. 2001. A novel human suspension culture packaging cell line for production of high-titre retroviral vectors. Gene Ther 8(9):697-703. (Pubitemid 32496431)
10. Chase LG, Firpo MT. 2007. Development of serum-free culture systems for human embryonic stem cells. Curr Opin Chem Biol 11(4):367-372. (Pubitemid 47302854)
11. Chen HF, Kuo HC, Chien CL, Shun CT, Yao YL, Ip PL, Chuang CY, Wang CC, Yang YS, Ho HN. 2007. Derivation, characterization and differentiation of human embryonic stem cells: Comparing serum-containing versus serum-free media and evidence of germ cell differentiation. Hum Reprod 22(2):567-577.
12. Coroadinha AS, Alves PM, Santos SS, Cruz PE, Merten OW, Carrondo MJ. 2006a. Retrovirus producer cell line metabolism: Implications on viral productivity. Appl Microbiol Biotechnol 72(6):1125-1135.
13. Coroadinha AS, Schucht R, Gama-Norton L, Wirth D, Hauser H, Carrondo MJ. 2006b. The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: Predictability and efficiency by transgene exchange. J Biotechnol 124(2):457-468. (Pubitemid 43912936)
14. Cosset FL, Takeuchi Y, Battini JL, Weiss RA, Collins MK. 1995. High-titer packaging cells producing recombinant retroviruses resistant to human serum. J Virol 69(12):7430-7436.
15. Edelstein ML, Abedi MR, Wixon J. 2007. Gene therapy clinical trials worldwide to 2007 - An update. J Gene Med 9(10):833-842. (Pubitemid 47543250)
16. Falkner E, Appl H, Eder C, Losert UM, Schoffl H, Pfaller W. 2006. Serum free cell culture: The free access online database. Toxicol In Vitro 20(3):395-400. (Pubitemid 43343771)
17. Gerin PA, Gilligan MG, Searle PF, Al-Rubeai M. 1999a. Improved titers of retroviral vectors from the human FLYRD18 packaging cell line in serum- and protein-free medium. Hum Gene Ther 10(12):1965-1974. (Pubitemid 29387910)
18. Gerin PA, Searle PF, Al-Rubeai M. 1999b. Production of retroviral vectors for gene therapy with the human packaging cell line FLYRD18. Biotechnol Prog 15(5):941-948. (Pubitemid 29475635)
19. Ghani K, Cottin S, Kamen A, Caruso M. 2007. Generation of a high-titer packaging cell line for the production of retroviral vectors in suspension and serum-free media. Gene Ther 14(24):1705-1711. (Pubitemid 350238447)
20. Laliberte JP, McGinnes LW, Peeples ME, Morrison TG. 2006. Integrity of membrane lipid rafts is necessary for the ordered assembly and release of infectious Newcastle disease virus particles. J Virol 80(21):10652-10662.
21. Le Doux JM, Davis HE, Morgan JR, Yarmush ML. 1999. Kinetics of retrovirus production and decay. Biotechnol Bioeng 63(6):654-662.
22. McTaggart S, Al-Rubeai M. 2000. Effects of culture parameters on the production of retroviral vectors by a human packaging cell line. Biotechnol Prog 16(5):859-865. (Pubitemid 30778886)
23. Merten OW. 2004. State-of-the-art of the production of retroviral vectors. J Gene Med 6(Suppl 1):S105-S124.
24. Mountain A. 2000. Gene therapy: The first decade. Trends Biotechnol 18(3):119-128.
25. Pizzato M, Merten OW, Blair ED, Takeuchi Y. 2001. Development of a suspension packaging cell line for production of high titre, serum-resistant murine leukemia virus vectors. Gene Ther 8(10):737-745. (Pubitemid 32509787)
26. Rawat SS, Viard M, Gallo SA, Rein A, Blumenthal R, Puri A. 2003. Modulation of entry of enveloped viruses by cholesterol and sphingolipids (Review). Mol Membr Biol 20(3):243-254. (Pubitemid 37046249)
27. Rodrigues T, Carrondo MJ, Alves PM, Cruz PE. 2007. Purification of retroviral vectors for clinical application: Biological implications and technological challenges. J Biotechnol 127(3):520-541. (Pubitemid 44827399)
28. Schucht R, Coroadinha AS, Zanta-Boussif MA, Verhoeyen E, Carrondo MJ, Hauser H, Wirth D. 2006. A new generation of retroviral producer cells: Predictable and stable virus production by Flp-mediated site-specific integration of retroviral vectors. Mol Ther 14(2):285-292. (Pubitemid 43964494)
29. Seth G, Philp RJ, Denoya CD, McGrath K, Stutzman-Engwall KJ, Yap M, Hu WS. 2005. Large-scale gene expression analysis of cholesterol dependence in NS0 cells. Biotechnol Bioeng 90(5):552-567. (Pubitemid 40733984)
30. Seth G, Ozturk M, Hu WS. 2006. Reverting cholesterol auxotrophy of NS0 cells by altering epigenetic gene silencing. Biotechnol Bioeng 93(4):820-827.
31. Shen BQCM, Clarke MF, Palsson BO. 1996. Kinetics of retroviral production from the amphotropic Psi-CRIP murine producer cell line. Cytotechnology 22(1-3):185-195.
32. Spector AA, Mathur SN, Kaduce TL, Hyman BT. 1980. Lipid nutrition and metabolism of cultured mammalian cells. Prog Lipid Res 19(3-4):155-186.
33. Stewart JC. 1980. Colorimetric determination of phospholipids with ammonium ferrothiocyanate. Anal Biochem 104(1):10-14.