Glioblastoma-derived spheroid cultures as an experimental model for analysis of EGFR anomalies

Journal of Neuro-Oncology

Volumn 102, Issue 3, 2011, Pages 395-407

  Witusik Perkowska, Monika ^a   Rieske, Piotr ^a   Hułas Bigoszewska, Krystyna ^a   Zakrzewska, Magdalena ^a   Stawski, Robert ^a   Kulczycka Wojdala, Dominika ^a   Bieńkowski, Michał ^a   Stoczyńska Fidelus, Ewelina ^a   Grešner, Sylwia M ^a   Piaskowski, Sylwester ^a   Jaskólski, Dariusz J ^a   Papierz, Wielisław ^a   Zakrzewski, Krzysztof ^b   Kolasa, MacIej ^c   Ironside, James W ^d   Liberski, Paweł P ^a  

^a MEDICAL UNIVERSITY OF LODZ   (Poland)

^b POLISH MOTHER S MEMORIAL HOSPITAL RESEARCH INSTITUTE   (Poland)

^c Memorial Copernicus Hospital in Lodz   (Poland)

^d UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Cell cultures; EGFR amplification; EGFRvIII; Glioblastoma; Spheroids

Indexed keywords

DNA; EPIDERMAL GROWTH FACTOR RECEPTOR 3; MESSENGER RNA;

ARTICLE; CHROMOSOME 7; CONTROLLED STUDY; EPIDERMAL GROWTH FACTOR RECEPTOR 3 GENE; EXPERIMENTAL MODEL; FLUORESCENCE IN SITU HYBRIDIZATION; GENE AMPLIFICATION; GENE DOSAGE; GENE LOCUS; GENE MUTATION; GENE OVEREXPRESSION; GENETIC GAIN; GENETIC VARIABILITY; GLIOBLASTOMA; HUMAN; HUMAN CELL; IN VITRO STUDY; ONCOGENE; QUANTITATIVE ANALYSIS; TUMOR CELL CULTURE; TUMOR SPHEROID;

ANIMALS; BRAIN NEOPLASMS; BROMODEOXYURIDINE; CELL ADHESION; CELL CYCLE; CELL PROLIFERATION; GENE EXPRESSION REGULATION, NEOPLASTIC; GLIOBLASTOMA; HUMANS; MODELS, ANIMAL; RECEPTOR, EPIDERMAL GROWTH FACTOR; RNA, MESSENGER; SOXB1 TRANSCRIPTION FACTORS; SPHEROIDS, CELLULAR; TIME FACTORS; TUMOR CELLS, CULTURED;

EID: 79958198736     PISSN: 0167594X     EISSN: 15737373     Source Type: Journal    
DOI: 10.1007/s11060-010-0352-0     Document Type: Article

References (34)

1. Layfield LJ, Willmore C, Tripp S, Jones C, Jensen RL (2006) Epidermal growth factor receptor gene amplification and protein expression in glioblastoma multiforme: prognostic significance and relationship to other prognostic factors. Appl Immunohistochem Mol Morphol 14(1):91-96
2. Ohgaki H, Kleihues P (2007) Genetic pathways to primary and secondary glioblastoma. Am J Pathol 170(5):1445-1453 (Pubitemid 47339290)
3. Leuraud P, Taillandier L, Aguirre-Cruz L, Medioni J, Crinière E, Marie Y, Dutrillaux AM, Kujas M, Duprez A, Delattre JY, Poupon MF, Sanson M (2003) Correlation between genetic alterations and growth of human malignant glioma xenografted in nude mice. Br J Cancer 89(12):2327-2332 (Pubitemid 38168019)
4. Pandita A, Aldape KD, Zadeh G, Guha A, James CD (2004) Contrasting in vivo and in vitro fates of glioblastoma cell subpopulations with amplified EGFR. Genes Chromosomes Cancer 39:29-36 (Pubitemid 37475250)
5. Giannini C, Sarkaria JN, Saito A, Uhm JH, Galanis E, Carlson BL, Schroeder MA, James CD (2005) Patient tumor EGFR and PDGFRA gene amplifications retained in an invasive intracranial xenograft model of glioblastoma multiforme. Neurooncology 7(2):164-176 (Pubitemid 40590516)
6. Gan HK, Kaye AH, Luwor RB (2009) The EGFRvIII variant in glioblastoma multiforme. J Clin Neurosci 16(6):748-754
7. Nagane M, Lin H, Cavenee WK, Huang HJ (2001) Aberrant receptor signaling in human malignant gliomas: mechanisms and therapeutic implications. Cancer Lett 162(Suppl):S17-S21
8. Mischel PS, Nelson SF, Cloughesy TF (2003) Molecular analysis of glioblastoma: pathway profiling and its implications for patient therapy. Cancer Biol Ther 2:242-247
9. Lee J, Kotliarova S, Kotliarov Y, Li A, Su Q, Donin NM, Pastorino S, Purow BW, Christopher N, Zhang W, Park JK, Fine HA (2006) Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines. Cancer Cell 9:391-403 (Pubitemid 43668737)
10. Inagaki A, Soeda A, Oka N, Kitajima H, Nakagawa J, Motohashi T, Kunisada T, Iwama T (2007) Long-term maintenance of brain tumor stem cell properties under at non-adherent and adherent culture conditions. Biochem Biophys Res Commun 361(3):586-592 (Pubitemid 47243620)
11. De Witt Hamer PC, Van Tilborg AA, Eijk PP, Sminia P, Troost D, Van Noorden CJ, Ylstra B, Leenstra S (2008) The genomic profile of human malignant glioma is altered early in primary cell culture and preserved in spheroids. Oncogene 27:2091-2096 (Pubitemid 351442747)
12. Rappa G, Mercapide J, Anzanello F, Prasmickaite L, Xi Y, Ju J, Fodstad O, Lorico A (2008) Growth of cancer cell lines under stem cell-like conditions has the potential to unveil therapeutic targets. Exp Cell Res 314:2110-2122
13. Fael Al-Mayhani TM, Ball SL, Zhao JW, Fawcett J, Ichimura K, Collins PV, Watts C (2009) An efficient method for derivation and propagation of glioblastoma cell lines that conserves the molecular profile of their original tumours. J Neurosci Methods 176(2):192-199
14. Rieske P, Golanska E, Zakrzewska M, Piaskowski S, Hulas- Bigoszewska K, Wolańczyk M, Szybka M, Witusik-Perkowska M, Jaskolski DJ, Zakrzewski K, Biernat W, Krynska B, Liberski PP (2009) Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors. BMC Cancer 14(9):54
15. Pfaffl MW, Horgan GW, Dempfle L (2002) Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30(9):e36
16. Yoshimoto K, Dang J, Zhu S, Nathanson D, Huang T, Dumont R, Seligson DB, Yong WH, Xiong Z, Rao N, Winther H, Chakravarti A, Bigner DD, Mellinghoff IK, Horvath S, Cavenee WK, Cloughesy TF, Mischel PS (2008) Development of a real-time RT-PCR assay for detecting EGFRvIII in glioblastoma samples. Clin Cancer Res 14(2):488-493 (Pubitemid 351226117)
17. Theys J, Jutten B, Dubois L, Rouschop KM, Chiu RK, Li Y, Paesmans K, Lambin P, Lammering G, Wouters BG (2009) The deletion mutant EGFRvIII significantly contributes to stress resistance typical for the tumour microenvironment. Radiother Oncol 92(3):399-404
18. Bell HS, Whittle IR, Walker M, Leaver HA, Wharton SB (2001) The development of necrosis and apoptosis in glioma: experimental findings using spheroid culture systems. Neuropathol Appl Neurobiol 27(4):291-304 (Pubitemid 32758617)
19. Stein AM, Demuth T, Mobley D, Berens M, Sander LM (2007) A mathematical model of glioblastoma tumor spheroid invasion in a three-dimensional in vitro experiment. Biophys J 92(1):356-365 (Pubitemid 46048443)
20. Günther HS, Schmidt NO, Phillips HS, Kemming D, Kharbanda S, Soriano R, Modrusan Z, Meissner H, Westphal M, Lamszus K (2008) Glioblastoma-derived stem cell-enriched cultures form distinct subgroups according to molecular and phenotypic criteria. Oncogene 27:2897-2909 (Pubitemid 351620450)
21. Gangemi RM, Griffero F, Marubbi D, Perera M, Capra MC, Malatesta P, Ravetti GL, Zona GL, Daga A, Corte G (2009) SOX2 silencing in glioblastoma tumor-initiating cells causes stop of proliferation and loss of tumorigenicity. Stem Cells 27(1):40-48
22. Bigner SH, Humphrey PA, Wong AJ, Vogelstein B, Mark J, Friedman HS, Bigner DD (1990) Characterization of the epidermal growth factor receptor in human glioma cell lines and xenografts. Cancer Res 50(24):8017-8022
23. Kunz-Schughart LA, Freyer JP, Hofstaedter F, Ebner R (2004) The use of 3-D cultures for high-throughput screening: the multicellular spheroid model. J Biomol Screen 9(4):273-285
24. Khaitan D, Chandna S, Arya MB, Dwarakanath BS (2006) Establishment and characterization of multicellular spheroids from a human glioma cell line; implications for tumor therapy. J Transl Med 4:12
25. Sampson JH, Archer GE, Mitchell DA, Heimberger AB, Bigner DD (2008) Tumor-specific immunotherapy targeting the EGFRvIII mutation in patients with malignant glioma. Semin Immunol 20(5):267-275
26. Martens T, Laabs Y, Günther HS, Kemming D, Zhu Z, Witte L, Hagel C, Westphal M, Lamszus K (2008) Inhibition of glioblastoma growth in a highly invasive nude mouse model can be achieved by targeting epidermal growth factor receptor but not vascular endothelial growth factor receptor-2. Clin Cancer Res 14(17):5447-5458
27. Huang PH, Mukasa A, Bonavia R, Flynn RA, Brewer ZE, Cavenee WK, Furnari FB, White FM (2007) Quantitative analysis of EGFRvIII cellular signaling networks reveals a combinatorial therapeutic strategy for glioblastoma. Proc Natl Acad Sci USA 104(31):12867-12872 (Pubitemid 47255246)
28. Kwanghee K, Brush JM, Watson PA, Cacalano NA, Iwamoto KS, McBride WH (2008) Epidermal growth factor receptor vIII expression in U87 glioblastoma cells alters their proteasome composition, function, and response to irradiation. Mol Cancer Res 6(3):426-434 (Pubitemid 351416533)
29. Lassman AB, Holland EC (2007) Incorporating molecular tools into clinical trials and treatment for gliomas? Curr Opin Neurol 20(6):708-711
30. Mladkova N, Chakravarti A (2009) Molecular profiling in glioblastoma: prelude to personalized treatment. Curr Oncol Rep 11(1):53-61
31. Kang CS, Pu PY, Li YH, Zhang ZY, Qiu MZ, Huang Q, Wang GX (2005) An in vitro study on the suppressive effect of glioma cell growth induced by plasmid-based small interference RNA (siRNA) targeting human epidermal growth factor receptor. J Neurooncol 74(3):267-273 (Pubitemid 41444492)
32. Han L, Zhang AL, Xu P, Yue X, Yang Y, Wang GX, Jia ZF, Pu PY, Kang CS (2009) Combination gene therapy with PTEN and EGFR siRNA suppresses U251 malignant glioma cell growth in vitro and in vivo. Med Oncol doi:10.1007/s12032-009-9295-8
33. Nagane M, Coufal F, Lin H, Bögler O, Cavenee WK, Huang HJ (1996) A common mutant epidermal growth factor receptor confers enhanced tumorigenicity on human glioblastoma cells by increasing proliferation and reducing apoptosis. Cancer Res 56:5079-5086 (Pubitemid 26374452)
34. Murat A, Migliavacca E, Gorlia T, Lambiv WL, Shay T, Hamou MF, de Tribolet N, Regli L, Wick W, Kouwenhoven MC, Hainfellner JA, Heppner FL, Dietrich PY, Zimmer Y, Cairncross JG, Janzer RC, Domany E, Delorenzi M, Stupp R, Hegi ME (2008) Stem cell-related "self-renewal" signature and high epidermal growth factor receptor expression associated with resistance to concomitant chemoradiotherapy in glioblastoma. J Clin Oncol 26(18):3015-3024