Live-cell imaging demonstrates rapid cargo exchange between lipid droplets in adipocytes

FEBS Letters

Volumn 585, Issue 12, 2011, Pages 1946-1950

  Somwar, Romel ^a   Roberts Jr , Charles T ^b,c   Varlamov, Oleg ^b  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^c OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

Adipocyte; Fatty acid; Fluorescent microscopy; FRAP; Lipid droplet; Obesity

Indexed keywords

FAT DROPLET; FATTY ACID;

ADIPOCYTE; ANIMAL CELL; ARTICLE; CELL COMMUNICATION; CELL CYCLE; CELL DIFFERENTIATION; CELL LABELING; CELL LEVEL; CELL TRANSPORT; CONTROLLED STUDY; FATTY ACID TRANSPORT; IMAGE ANALYSIS; LIPID ANALYSIS; LIPID COMPOSITION; LIPID METABOLISM; LIPID STORAGE; LIVE CELL MICROSCOPY; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL;

3T3-L1 CELLS; ADIPOCYTES; ANIMALS; BIOLOGICAL TRANSPORT; CYTOPLASM; FATTY ACIDS; FLUORESCENT DYES; LIPID METABOLISM; MICE; MICROSCOPY, FLUORESCENCE;

EID: 79958081139     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1016/j.febslet.2011.05.016     Document Type: Article

References (37)

1. S. Martin, and R.G. Parton Lipid droplets: a unified view of a dynamic organelle Nat. Rev. Mol. Cell Biol. 7 2006 373 378
2. H. Doege, and A. Stahl Protein-mediated fatty acid uptake: novel insights from in vivo models Physiology (Bethesda) 21 2006 259 268 (Pubitemid 44156858)
3. A. Stahl, J.G. Evans, S. Pattel, D. Hirsch, and H.F. Lodish Insulin causes fatty acid transport protein translocation and enhanced fatty acid uptake in adipocytes Dev. Cell 2 2002 477 488 (Pubitemid 34436575)
4. K.K. Buhman, H.C. Chen, and R.V. Farese Jr. The enzymes of neutral lipid synthesis J. Biol. Chem. 276 2001 40369 40372
5. G. Haemmerle Hormone-sensitive lipase deficiency in mice causes diglyceride accumulation in adipose tissue, muscle, and testis J. Biol. Chem. 277 2002 4806 4815 (Pubitemid 34968514)
6. R. Zimmermann Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase Science 306 2004 1383 1386 (Pubitemid 39532517)
7. M. Schweiger Adipose triglyceride lipase and hormone-sensitive lipase are the major enzymes in adipose tissue triacylglycerol catabolism J. Biol. Chem. 281 2006 40236 40241 (Pubitemid 46041828)
8. N.E. Wolins, D.L. Brasaemle, and P.E. Bickel A proposed model of fat packaging by exchangeable lipid droplet proteins FEBS Lett. 580 2006 5484 5491 (Pubitemid 44465908)
9. M. Digel, R. Ehehalt, and J. Fullekrug Lipid droplets lighting up: insights from live microscopy FEBS Lett. 584 2010 2168 2175
10. S. Martin, K. Driessen, S.J. Nixon, M. Zerial, and R.G. Parton Regulated localization of Rab18 to lipid droplets: effects of lipolytic stimulation and inhibition of lipid droplet catabolism J. Biol. Chem. 280 2005 42325 42335 (Pubitemid 43023204)
11. M. Beller, D. Riedel, L. Jansch, G. Dieterich, J. Wehland, H. Jackle, and R.P. Kuhnlein Characterization of the drosophila lipid droplet subproteome Mol. Cell. Proteomics 5 2006 1082 1094 (Pubitemid 43985932)
12. N.E. Wolins, B.K. Quaynor, J.R. Skinner, M.J. Schoenfish, A. Tzekov, and P.E. Bickel S3-12, Adipophilin, and TIP47 package lipid in adipocytes J. Biol. Chem. 280 2005 19146 19155 (Pubitemid 41379621)
13. N.E. Wolins, J.R. Skinner, M.J. Schoenfish, A. Tzekov, K.G. Bensch, and P.E. Bickel Adipocyte protein S3-12 coats nascent lipid droplets J. Biol. Chem. 278 2003 37713 37721 (Pubitemid 37175296)
14. P. Bostrom SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity Nat. Cell Biol. 9 2007 1286 1293 (Pubitemid 350042360)
15. L. Kuerschner, C. Moessinger, and C. Thiele Imaging of lipid biosynthesis: how a neutral lipid enters lipid droplets Traffic 9 2008 338 352 (Pubitemid 351188994)
16. H.A. Rinia, K.N. Burger, M. Bonn, and M. Muller Quantitative label-free imaging of lipid composition and packing of individual cellular lipid droplets using multiplex CARS microscopy Biophys. J. 95 2008 4908 4914
17. J.R. Skinner, T.M. Shew, D.M. Schwartz, A. Tzekov, C.M. Lepus, N.A. Abumrad, and N.E. Wolins Diacylglycerol enrichment of endoplasmic reticulum or lipid droplets recruits perilipin 3/TIP47 during lipid storage and mobilization J. Biol. Chem. 284 2009 30941 30948
18. J. Spandl, D.J. White, J. Peychl, and C. Thiele Live cell multicolor imaging of lipid droplets with a new dye, LD540 Traffic 10 2009 1579 1584
19. O. Varlamov, R. Somwar, A. Cornea, P. Kievit, K.L. Grove, and C.T. Roberts Jr. Single-cell analysis of insulin-regulated fatty acid uptake in adipocytes Am. J. Physiol. Endocrinol. Metab. 299 2010 E486 E496
20. D. Steinberg Fatty acid mobilization - mechanisms of regulation and metabolic consequences Biochem. Soc. Symp. 24 1963 111 143
21. E.A. Newsholme, and W. Gevers Control of glycolysis and gluconeogenesis in liver and kidney cortex Vitam. Horm. 25 1967 1 87
22. B. Brooks, J.R. Arch, and E.A. Newsholme Effects of hormones on the rate of the triacylglycerol/fatty acid substrate cycle in adipocytes and epididymal fat pads FEBS Lett. 146 1982 327 330 (Pubitemid 13252918)
23. J. Liao, R. Sportsman, J. Harris, and A. Stahl Real-time quantification of fatty acid uptake using a novel fluorescence assay J. Lipid Res. 46 2005 597 602 (Pubitemid 43109796)
24. P.E. Scherer, P.E. Bickel, M. Kotler, and H.F. Lodish Cloning of cell-specific secreted and surface proteins by subtractive antibody screening Nat. Biotechnol. 16 1998 581 586 (Pubitemid 28269819)
25. D.L. Brasaemle, T. Barber, N.E. Wolins, G. Serrero, E.J. Blanchette-Mackie, and C. Londos Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein J. Lipid Res. 38 1997 2249 2263 (Pubitemid 27495384)
26. H.W. Heid, R. Moll, I. Schwetlick, H.R. Rackwitz, and T.W. Keenan Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases Cell Tissue Res. 294 1998 309 321 (Pubitemid 28486407)
27. N.E. Wolins, B. Rubin, and D.L. Brasaemle TIP47 associates with lipid droplets J. Biol. Chem. 276 2001 5101 5108
28. C. Thiele, and J. Spandl Cell biology of lipid droplets Curr. Opin. Cell Biol. 20 2008 378 385
29. L. Andersson PLD1 and ERK2 regulate cytosolic lipid droplet formation J. Cell Sci. 119 2006 2246 2257 (Pubitemid 43960314)
30. A. Marcinkiewicz, D. Gauthier, A. Garcia, and D.L. Brasaemle The phosphorylation of serine 492 of perilipin a directs lipid droplet fragmentation and dispersion J. Biol. Chem. 281 2006 11901 11909 (Pubitemid 43855452)
31. V. Puri, S. Konda, S. Ranjit, M. Aouadi, A. Chawla, M. Chouinard, A. Chakladar, and M.P. Czech Fat-specific protein 27, a novel lipid droplet protein that enhances triglyceride storage J. Biol. Chem. 282 2007 34213 34218 (Pubitemid 350159455)
32. H. Miyoshi Control of adipose triglyceride lipase action by serine 517 of perilipin A globally regulates protein kinase A-stimulated lipolysis in adipocytes J. Biol. Chem. 282 2007 996 1002 (Pubitemid 47076543)
33. B. Kadereit Evolutionarily conserved gene family important for fat storage Proc. Natl. Acad. Sci. USA 105 2008 94 99
34. Y. Guo Functional genomic screen reveals genes involved in lipid-droplet formation and utilization Nature 453 2008 657 661 (Pubitemid 351769298)
35. J. Lippincott-Schwartz, N. Altan-Bonnet, and G.H. Patterson Photobleaching and photoactivation: following protein dynamics in living cells Nat. Cell Biol. Suppl 2003 S7 S14
36. J.E. Rothman The golgi apparatus: two organelles in tandem Science 213 1981 1212 1219 (Pubitemid 11019607)
37. G. Sweeney, R. Somwar, T. Ramlal, A. Volchuk, A. Ueyama, and A. Klip An inhibitor of p38 mitogen-activated protein kinase prevents insulin-stimulated glucose transport but not glucose transporter translocation in 3T3-L1 adipocytes and L6 myotubes J. Biol. Chem. 274 1999 10071 10078