Comparative gene expression analysis throughout the life cycle of Leishmania braziliensis: Diversity of expression profiles among clinical isolates

PLoS Neglected Tropical Diseases

Volumn 5, Issue 5, 2011, Pages

  Adaui, Vanessa ^a,b   Castillo, Denis ^a,c   Zimic, Mirko ^c   Gutierrez, Andres ^c   Decuypere, Saskia ^b   Vanaerschot, Manu ^b,d   de Doncker, Simonne ^b   Schnorbusch, Kathy ^b,d   Maes, Ilse ^b   van der Auwera, Gert ^b   Maes, Louis ^d   Llanos Cuentas, Alejandro ^a   Arevalo, Jorge ^a,c   Dujardin, Jean Claude ^b,d  

^a INSTITUTO DE MEDICINA TROPICAL ALEXANDER VON HUMBOLDT   (Peru)

^b INSTITUTE OF TROPICAL MEDICINE   (Belgium)

^c UNIVERSIDAD PERUANA CAYETANO HEREDIA   (Peru)

^d UNIVERSITY OF ANTWERP   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

AMASTIGOTE; ANIMAL CELL; ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; CYTOSKELETON; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETIC VARIABILITY; HOUSEKEEPING GENE; LEISHMANIA BRAZILIENSIS; LIFE CYCLE ASSESSMENT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE ISOLATION; PHENOTYPE; PROMASTIGOTE; PROTEIN TRANSPORT; REAL TIME POLYMERASE CHAIN REACTION; RNA METABOLISM; SEQUENCE ALIGNMENT; BIOSYNTHESIS; CHEMISTRY; DNA SEQUENCE; GENETICS; GROWTH, DEVELOPMENT AND AGING; MOLECULAR GENETICS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

PROTOZOAL DNA; PROTOZOAL PROTEIN;

DNA, PROTOZOAN; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; LEISHMANIA BRAZILIENSIS; MOLECULAR SEQUENCE DATA; PROTOZOAN PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

EID: 79958015495     PISSN: 19352727     EISSN: 19352735     Source Type: Journal    
DOI: 10.1371/journal.pntd.0001021     Document Type: Article

References (24)

1. Barak E, Amin-Spector S, Gerliak E, Goyard S, Holland N, et al. (2005) Differentiation of Leishmania donovani in host-free system: analysis of signal perception and response. Mol Biochem Parasitol 141: 99-108.
2. Haile S, Papadopoulou B, (2007) Developmental regulation of gene expression in trypanosomatid parasitic protozoa. Curr Opin Microbiol 10: 569-577.
3. Saxena A, Lahav T, Holland N, Aggarwal G, Anupama A, et al. (2007) Analysis of the Leishmania donovani transcriptome reveals an ordered progression of transient and permanent changes in gene expression during differentiation. Mol Biochem Parasitol 152: 53-65.
4. Alcolea PJ, Alonso A, Gómez MJ, Moreno I, Domínguez M, et al. (2010) Transcriptomics throughout the life cycle of Leishmania infantum: high down-regulation rate in the amastigote stage. Int J Parasitol 40: 1497-1516.
5. Depledge DP, Evans KJ, Ivens AC, Aziz N, Maroof A, et al. (2009) Comparative expression profiling of Leishmania: modulation in gene expression between species and in different host genetic backgrounds. PLoS Negl Trop Dis 3: e476.
6. Adaui V, Schnorbusch K, Zimic M, Gutiérrez A, Decuypere S, et al. (2011) Comparison of gene expression patterns among Leishmania braziliensis clinical isolates showing a different in vitro susceptibility to pentavalent antimony. Parasitology 138: 183-193.
7. Decuypere S, Vanaerschot M, Rijal S, Yardley V, Maes L, et al. (2008) Gene expression profiling of Leishmania (Leishmania) donovani: overcoming technical variation and exploiting biological variation. Parasitology 135: 183-194.
8. Yardley V, Ortuño N, Llanos-Cuentas A, Chappuis F, De Doncker S, et al. (2006) American tegumentary leishmaniasis: Is antimonial treatment outcome related to parasite drug susceptibility? J Infect Dis 194: 1168-1175.
9. Fraga J, Montalvo AM, De Doncker S, Dujardin JC, Van der Auwera G, (2010) Phylogeny of Leishmania species based on the heat-shock protein 70 gene. Infect Genet Evol 10: 238-245.
10. Garcia L, Kindt A, Bermudez H, Llanos-Cuentas A, De Doncker S, et al. (2004) Culture-independent species typing of neotropical Leishmania for clinical validation of a PCR-based assay targeting heat shock protein 70 genes. J Clin Microbiol 42: 2294-2297.
11. da Silva LA, de Sousa Cdos S, da Graça GC, Porrozzi R, Cupolillo E, (2010) Sequence analysis and PCR-RFLP profiling of the hsp70 gene as a valuable tool for identifying Leishmania species associated with human leishmaniasis in Brazil. Infect Genet Evol 10: 77-83.
12. Montalvo AM, Fraga J, Monzote L, Montano I, De Doncker S, et al. (2010) Heat-shock protein 70 PCR-RFLP: a universal simple tool for Leishmania species discrimination in the New and Old World. Parasitology 137: 1159-1168.
13. Puentes F, Diaz D, Hoya RD, Gutíerrez JA, Lozano JM, et al. (2000) Cultivation and characterization of stable Leishmania guyanensis complex axenic amastigotes derived from infected U937 cells. Am J Trop Med Hyg 63: 102-110.
14. Teixeira MC, de Jesus Santos R, Sampaio RB, Pontes-de-Carvalho L, dos-Santos WL, (2002) A simple and reproducible method to obtain large numbers of axenic amastigotes of different Leishmania species. Parasitol Res 88: 963-968.
15. Gamboa D, Torres K, De Doncker S, Zimic M, Arevalo J, et al. (2008) Evaluation of an in vitro and in vivo model for experimental infection with Leishmania (Viannia) braziliensis and L. (V.) peruviana. Parasitology 135: 319-326.
16. Decuypere S, Rijal S, Yardley V, De Doncker S, Laurent T, et al. (2005) Gene expression analysis of the mechanism of natural Sb(V) resistance in Leishmania donovani isolates from Nepal. Antimicrob Agents Chemother 49: 4616-4621.
17. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, et al. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: RESEARCH0034.
18. Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J, (2007) qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol 8: R19.
19. McCarthy DJ, Smyth GK, (2009) Testing significance relative to a fold-change threshold is a TREAT. Bioinformatics 25: 765-771.
20. Holzer TR, McMaster WR, Forney JD, (2006) Expression profiling by whole-genome interspecies microarray hybridization reveals differential gene expression in procyclic promastigotes, lesion-derived amastigotes, and axenic amastigotes in Leishmania mexicana. Mol Biochem Parasitol 146: 198-218.
21. Rochette A, Raymond F, Corbeil J, Ouellette M, Papadopoulou B, (2009) Whole-genome comparative RNA expression profiling of axenic and intracellular amastigote forms of Leishmania infantum. Mol Biochem Parasitol 165: 32-47.
22. Ashutosh, Sundar S, Goyal N, (2007) Molecular mechanisms of antimony resistance in Leishmania. J Med Microbiol 56: 143-153.
23. Rochette A, Raymond F, Ubeda JM, Smith M, Messier N, et al. (2008) Genome-wide gene expression profiling analysis of Leishmania major and Leishmania infantum developmental stages reveals substantial differences between the two species. BMC Genomics 9: 255.
24. t'Kindt R, Scheltema RA, Jankevics A, Brunker K, Rijal S, et al. (2010) Metabolomics to unveil and understand phenotypic diversity between pathogen populations. PLoS Negl Trop Dis 4: e904 doi:10.1371/journal.pntd.0000904.