Cultivation and characterization of stable Leishmania guyanensis complex axenic amastigotes derived from infected U937 cells

American Journal of Tropical Medicine and Hygiene

Volumn 63, Issue 1-2, 2000, Pages 102-110

  Puentes, F ^a   Diaz, D ^a   Hoya, R D ^a   Gutierrez J A ^a   Lozano, J M ^a   Patarroyo, M E ^a   Moreno, A ^a  

^a Hospital San Juan de Dios   (Colombia)

Author keywords

[No Author keywords available]

Indexed keywords

AMASTIGOTE; ANIMAL CELL; ARTICLE; CELL CULTURE; CELL LINE; GENE CONTROL; IN VITRO STUDY; LEISHMANIA; LIFE CYCLE; MACROPHAGE; NONHUMAN; PARASITE VIRULENCE; PH; PROMASTIGOTE; TEMPERATURE;

ANIMALIA; LEISHMANIA GUYANENSIS; LEISHMANIA PANAMENSIS; PROTOZOA;

EID: 0034441836     PISSN: 00029637     EISSN: None     Source Type: Journal    
DOI: 10.4269/ajtmh.2000.63.102     Document Type: Article

References (45)

1. Leishmania major Hsp100 is required chiefly in the mammalian stage of the parasite
2. Loss of virulence in Leishmania donovani deficient in an amastigote-specific protein, A2
3. Identification and overexpression of the A2 amastigote-specific protein in Leishmania donovani
4. 2+ pool content and signaling and expression of a calcium pump are linked to virulence in Leishmania mexicana amazonesis amastigotes
5. Evidence from disruption of the lmcpb gene array of Leishmania mexicana that cysteine proteinases are virulence factors
6. Lipophosphoglycan blocks attachment of Leishmania major amastigotes to macrophages
7. Kinetics of entry of virulent and avirulent strains of Leishmania donovani into macrophages: A possible role of virulence molecules (gp63 and LPG)
8. Leishmania mexicana: Purine metabolism in promastigotes, axenic amastigotes, and amastigotes derived from Vero cells
9. Proteophosphoglycan secreted by Leishmania mexicana amastigotes causes vacuole formation in macrophages
10. Glucose transport in amastigotes and promastigotes of Leishmania mexicana mexicana
11. Characterization of developmentally-regulated activities in axenic amastigotes of Leishmania donovani
12. Genomic organization and expression of a differentially-regulated gene family from Leishmania major
13. Cloning of genes and expression and antigenicity analysis of the Leishmania infantum KMP-11 protein
14. The Leishmania promastigote surface antigen 2 complex is differentially expressed during the parasite life cycle
15. Expression of heat shock protein 83 in Leishmania is regulated post-transcriptionally
16. Leishmania donovani heat shock protein 100. Characterization and function in amastigote stage differentiation
17. Histone H1 expression varies during the Leishmania major life cycle
18. Developmental gene expression in Leishmania donovani: Differential cloning and analysis of an amastigote-stage-specific gene
19. Characterization of a second protein encoded by the differentially regulated LmcDNA 16 gene family of Leishmania major
20. Developmental regulation of spliced leader RNA gene in Leishmania donovani amastigotes is mediated by specific polyadenylation
21. Identification of two distinct cysteine proteinase genes of Leishmania pifanoi axenic amastigotes using the polymerase chain reaction
22. Molecular cloning, characterization and overexpression of two distinct cysteine protease cDNAs from Leishmania donovani chagasi
23. In vitro maintenance of Leishmania amastigotes directly from lesions: Advantages and limitations
24. Leishmania donovani: Long-term culture of axenic amastigotes at 37°C
25. Axenic cultivation and partial characterization of Leishmania braziliensis amastigote-like stages
26. Extracellular cultivation and morphological characterization of amastigote-like forms of Leishmania panamensis and L. braziliensis
27. Extracellular amastigote-like forms of Leishmania panamensis and L. braziliensis. II. Stage- and species-specific monoclonal antibodies
28. Leishmania mexicana: Serial cultivation of intracellular stages in a cell-free medium
29. Leishmania amazonensis: Cultivation and characterization of axenic amastigote-like organisms
30. Leishmania pifanoi amastigote antigen P-4: Epitopes involved in T-cell responsiveness in human cutaneous leishmaniasis
31. Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms
32. Leishmania: Fine mapping of the Leishmanolysin molecule's conserved core domains involved in binding and internalization
33. Continuous cultures of fused cells secreting antibody of predefined specificity
34. Temperature-induced in vitro transformation of Leishmania mexicana. I. Ultrastructural comparison of culture-transformed and intracellular amastigotes
35. Developmental life cycle of Leishmania: Cultivation and characterization of cultured extracellular amastigotes
36. Diagnosis of New World leishmaniasis: Specific detection of species of the Leishmania braziliensis complex by amplification of kinetoplast DNA
37. Human monocyte infection by Leishmania (Viannia) panamensis. Role of complement receptors and correlation of susceptibility in vitro with clinical phenotype
38. Cloning and characterization of differentially expressed genes from in vitro-grown 'amastigotes' of Leishmania donovani
39. Infectivity of the subspecies of the Leishmania braziliensis complex in vivo and in vitro
40. Developmental changes in the expression of Leishmania chagasi gp63 and heat shock protein in a human macrophage cell line
41. Growth of Leishmania donovani amastigotes in the continuous human macrophage cell line U937: Studies of drug efficacy and metabolism
42. Mixed infection of human U-937 cells by two different species of Leishmania
43. Characterization of an antigen from Leishmania amazonensis amastigotes able to elicit protective responses in a murine model
44. Leishmania pifanoi amastigote antigens protect mice against cutaneous leishmaniasis