Heterologous overexpression and mutagenesis of the human bile salt export pump (ABCB11) using DREAM (Directed REcombination-Assisted Mutagenesis)

PLoS ONE

Volumn 6, Issue 5, 2011, Pages

  Stindt, Jan ^a   Ellinger, Philipp ^a   Stross, Claudia ^a   Keitel, Verena ^a   Häussinger, Dieter ^a   Smits, Sander H J ^a   Kubitz, Ralf ^a   Schmitt, Lutz ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ABC TRANSPORTER; BILE SALT EXPORT PUMP; COMPLEMENTARY DNA; UNCLASSIFIED DRUG; ABCB11 PROTEIN, HUMAN;

ANALYTIC METHOD; ARTICLE; BSEP GENE; CONTROLLED STUDY; DIRECTED RECOMBINATION ASSISTED MUTAGENESIS; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE FUNCTION; GENE LOCATION; GENE MUTATION; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; CELL STRAIN HEK293; FLOW CYTOMETRY; GENETICS; METABOLISM; PICHIA; POLYMERASE CHAIN REACTION;

MAMMALIA; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

ATP-BINDING CASSETTE TRANSPORTERS; FLOW CYTOMETRY; HEK293 CELLS; HUMANS; MUTAGENESIS, SITE-DIRECTED; PICHIA; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

EID: 79957845495     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0020562     Document Type: Article

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