Use of chemical chaperones in the yeast Saccharomyces cerevisiae to enhance heterologous membrane protein expression: High-yield expression and purification of human P-glycoprotein

Archives of Biochemistry and Biophysics

Volumn 376, Issue 1, 2000, Pages 34-46

  Figler, Robert A ^a   Omote, Hiroshi ^a   Nakamoto, Robert K ^a   Al Shawi, Marwan K ^a  

^a UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

Author keywords

ABC transporters; Chemical chaperones; Glycerol; Heterologous expression; Membrane proteins; P glycoprotein; Saccharomyces cerevisiae; Yeast

Indexed keywords

ABC TRANSPORTER; CHAPERONE; GLYCEROL; GLYCOPROTEIN P; MEMBRANE PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; ENZYME ACTIVITY; GLYCOSYLATION; LIPID COMPOSITION; MULTIDRUG RESISTANCE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEOLIPOSOME; SACCHAROMYCES CEREVISIAE; YEAST;

MAMMALIA; SACCHAROMYCES CEREVISIAE;

EID: 0034176255     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1006/abbi.2000.1712     Document Type: Article

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