Expression of functional multidrug-resistance protein 1 in Saccharomyces cerevisiae: Effects of N- and C-terminal affinity tags

Biochemical and Biophysical Research Communications

Volumn 306, Issue 3, 2003, Pages 644-649

  Lee, Sung Haeng ^a   Altenberg, Guillermo A ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

ABC proteins; Affinity tag; EGFP; FLAG; Green fluorescent protein; Membrane protein expression; Multidrug resistance; Protein purification

Indexed keywords

CHAPERONE; EPITOPE; GLYCEROL; HISTIDINE; HYBRID PROTEIN; MULTIDRUG RESISTANCE PROTEIN 1; PROTON PUMP; GLYCOPROTEIN P;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; GENE EXPRESSION SYSTEM; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FUNCTION; SACCHAROMYCES CEREVISIAE; YEAST; YEAST EXPRESSION SYSTEM; AFFINITY LABELING; ANIMAL; EVALUATION; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; GENETICS; HUMAN; METABOLISM; PLASMID;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

AFFINITY LABELS; ANIMALS; GENE EXPRESSION REGULATION, FUNGAL; HUMANS; P-GLYCOPROTEIN; PLASMIDS; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; TRANSFECTION;

EID: 0042131621     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(03)01029-5     Document Type: Article

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