A novel method for the production of in vivo-assembled, recombinant Escherichia coli RNA polymerase lacking the α C-terminal domain

Protein Science

Volumn 20, Issue 6, 2011, Pages 986-995

  Twist, Kelly Anne ^a   Husnain, Seyyed I ^b   Franke, Josef D ^a   Jain, Deepti ^a   Campbell, Elizabeth A ^a   Nickels, Bryce E ^c   Thomas, Mark S ^b   Darst, Seth A ^a   Westblade, Lars F ^a,d  

^a ROCKEFELLER UNIVERSITY   (United States)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

^c RUTGERS UNIVERSITY   (United States)

^d WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

subunit; Macromolecular complex; Purification; RNA polymerase

Indexed keywords

ENZYME VARIANT; RECOMBINANT ENZYME; RNA POLYMERASE;

ARTICLE; BIOCHEMISTRY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENETIC TRANSCRIPTION; IN VIVO STUDY; MUTANT; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; SEQUENCE DELETION; UP-REGULATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 79956226261     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.622     Document Type: Article

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