Purification of Escherichia coli RNA polymerase using a self-cleaving elastin-like polypeptide tag

Protein Science

Volumn 19, Issue 6, 2010, Pages 1243-1252

  Fong, Baley A ^a   Gillies, Alison R ^a   Ghazi, Iraj ^a   LeRoy, Gary ^b   Lee, Kathleen C ^a   Westblade, Lars F ^c   Wood, David W ^a,d  

^a Princeton University   (United States)

^b PRINCETON UNIVERSITY   (United States)

^c ROCKEFELLER UNIVERSITY   (United States)

^d Ohio State University   (United States)

Author keywords

ELP; Intein; Multisubunit enzyme; Purification; RNA polymerase

Indexed keywords

CASPASE RECRUITMENT DOMAIN PROTEIN 4; ELASTIN; POLYETHYLENE; POLYETHYLENEIMINE; POLYNUCLEOTIDE; POLYPEPTIDE; RECOMBINANT ENZYME; RNA POLYMERASE;

ALPHA CHAIN; ARTICLE; BETA CHAIN; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SUBUNIT; ESCHERICHIA COLI; NONHUMAN; PRECIPITATION; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN EXPRESSION;

CHEMICAL PRECIPITATION; CLONING, MOLECULAR; DNA-DIRECTED RNA POLYMERASES; ELASTIN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; POLYETHYLENE GLYCOLS; POLYETHYLENEIMINE;

ESCHERICHIA COLI;

EID: 77952736311     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.403     Document Type: Article

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