Structure of the Escherichia coli RNA polymerase α subunit amino- terminal domain

Science

Volumn 281, Issue 5374, 1998, Pages 262-266

  Zhang, Gongyi ^a   Darst, Seth A ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CRYSTAL STRUCTURE; ENZYME ANALYSIS; ENZYME STRUCTURE; ESCHERICHIA COLI; PRIORITY JOURNAL; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RNA POLYMERASE II; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ALIGNMENT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 0032504132     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.281.5374.262     Document Type: Article

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39. R45A and for preliminary crystallization trials. We thank R. Ebright for plasmid DNA encoding E. coli RNAP α, T. Muir for assistance with mass spectroscopy, J. Bonanno for assistance with x-ray measurements, and W. A. Hendrickson and C. Ogata for access to and support at beamline X4A, which is funded by the Howard Hughes Medical Institute at the Brookhaven National Laboratory. We thank L. Niu, J. Goldberg, and D. Jeruzalmi for suggestions on refinement and data analysis. G.Z. was supported in part by a National Research Service Award (NIH GM19441-01). S.A.D. is a Pew Scholar in the Biomedical Sciences. This work was supported in part by grants to S.A.D. from the Irma T. Hirschl Trust, the Pew Foundation, and NIH (GM53759).