Direct effects of iodothyronines on excess fat storage in rat hepatocytes

Journal of Hepatology

Volumn 54, Issue 6, 2011, Pages 1230-1236

  Grasselli, Elena ^a   Voci, Adriana ^a   Canesi, Laura ^a   De Matteis, Rita ^b   Goglia, Fernando ^c   Cioffi, Federica ^d   Fugassa, Emilia ^a   Gallo, Gabriella ^a   Vergani, Laura ^a  

^a UNIVERSITY OF GENOA   (Italy)

^b UNIVERSITY OF URBINO   (Italy)

^c UNIVERSITY OF SANNIO   (Italy)

^d SECOND UNIVERSITY OF NAPLES   (Italy)

Author keywords

3,5 l diiodothyronine (T2); Acyl CoA oxidase (AOX); Adipocyte differentiation related protein (ADRP); Antioxidant enzymes; Fatty hepatocytes

Indexed keywords

ACYL COENZYME A OXIDASE; ADIPOPHILIN; CATALASE; DIIODOTHYRONINE; FAT DROPLET; FATTY ACID; LIOTHYRONINE; MESSENGER RNA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR DELTA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; SUPEROXIDE DISMUTASE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; FATTY ACID OXIDATION; FATTY LIVER; GENE EXPRESSION; LIPID ANALYSIS; LIPID STORAGE; LIVER CELL; MALE; MORPHOMETRICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REDUCTION;

ACYL-COA OXIDASE; ANIMALS; CATALASE; CELLS, CULTURED; DIIODOTHYRONINES; FATTY LIVER; GENE EXPRESSION; HEPATOCYTES; LIPID METABOLISM; OLEIC ACID; PALMITATES; PPAR ALPHA; PPAR DELTA; PPAR GAMMA; RATS; RNA, MESSENGER; SUPEROXIDE DISMUTASE; TRIIODOTHYRONINE;

EID: 79956076145     PISSN: 01688278     EISSN: 01688278     Source Type: Journal    
DOI: 10.1016/j.jhep.2010.09.027     Document Type: Article

References (46)

1. L.A. Adams, and P. Angulo Recent concepts in non-alcoholic fatty liver disease Diabet Med 22 2005 1129 1133 (Pubitemid 41224168)
2. L.A. Adams, and P. Angulo Treatment of non-alcoholic fatty liver disease Postgrad Med J 82 2006 315 322 (Pubitemid 43780662)
3. K.L. Donnelly, C.I. Smith, S.J. Schwarzenberg, J. Jessurun, M.D. Boldt, and E.J. Parks Sources of fatty acids stored in liver and secreted via lipoproteins in patients with nonalcoholic fatty liver disease J Clin Invest 115 2005 1343 1351 (Pubitemid 40629054)
4. M.W. Bradbury Lipid metabolism and liver inflammation. I. Hepatic fatty acid uptake: possible role in steatosis Am J Physiol Gastrointest Liver Physiol 290 2006 G194 198
5. E. Grasselli, A. Voci, C. Pesce, L. Canesi, E. Fugassa, and G. Gallo PAT protein mRNA expression in primary rat hepatocytes: effects of exposure to fatty acids Int J Mol Med 25 2010 505 512
6. W. Motomura, M. Inoue, T. Ohtake, N. Takahashi, M. Nagamine, and S. Tanno Up-regulation of ADRP in fatty liver in human and liver steatosis in mice fed with high fat diet Biochem Biophys Res Commun 340 2006 1111 1118 (Pubitemid 43083393)
7. P. Targett-Adams, M.J. McElwee, E. Ehrenborg, M.C. Gustafsson, C.N. Palmer, and J. McLauchlan A PPAR response element regulates transcription of the gene for human adipose differentiation-related protein Biochim Biophys Acta 1728 2005 95 104 (Pubitemid 40409959)
8. A. Lanni, M. Moreno, A. Lombardi, and F. Goglia Thyroid hormone and uncoupling proteins FEBS Lett 543 2003 5 10 (Pubitemid 36577217)
9. J.E. Silva The thermogenic effect of thyroid hormone and its clinical implications Ann Intern Med 139 2003 205 213 (Pubitemid 38124603)
10. M. Cimmino, F. Mion, F. Goglia, Y. Minaire, and A. Geloen Demonstration of in vivo metabolic effects of 3, 5-di-iodothyronine J Endocrinol 149 1996 319 325 (Pubitemid 26173542)
11. M. Moreno, A. Lombardi, P. Lombardi, F. Goglia, and A. Lanni Effect of 3, 5-diiodo-L-thyronine on thyroid stimulating hormone and growth hormone serum levels in hypothyroid rats Life Sci 62 1998 2369 2377 (Pubitemid 28273143)
12. A. Lanni, M. Moreno, A. Lombardi, P. de Lange, E. Silvestri, and M. Ragni 3, 5-diiodo-L-thyronine powerfully reduces adiposity in rats by increasing the burning of fats Faseb J 19 2005 1552 1554 (Pubitemid 41279028)
13. E. Grasselli, L. Canesi, A. Voci, R. De Matteis, I. Demori, and E. Fugassa Effects of 3, 5-diiodo-L-thyronine administration on the liver of high fat diet-fed rats Exp Biol Med (Maywood) 233 2008 549 557 (Pubitemid 351657356)
14. M.P. Mollica, L. Lionetti, M. Moreno, A. Lombardi, P. De Lange, and A. Antonelli 3, 5-diiodo-l-thyronine, by modulating mitochondrial functions, reverses hepatic fat accumulation in rats fed a high-fat diet J Hepatol 51 2009 363 370
15. A.M. Giudetti, M. Leo, M.J. Geelen, and G.V. Gnoni Short-term stimulation of lipogenesis by 3, 5-L-diiodothyronine in cultured rat hepatocytes Endocrinology 146 2005 3959 3966 (Pubitemid 41175767)
16. H.N. Suh, H.T. Huong, C.H. Song, J.H. Lee, and H.J. Han Linoleic acid stimulates gluconeogenesis via Ca2+/PLC, cPLA2, and PPAR pathways through GPR40 in primary cultured chicken hepatocytes Am J Physiol Cell Physiol 295 2008 C1518 1527
17. M. Vinciguerra, A. Sgroi, C. Veyrat-Durebex, L. Rubbia-Brandt, L.H. Buhler, and M. Foti Unsaturated fatty acids inhibit the expression of tumor suppressor phosphatase and tensin homolog (PTEN) via microRNA-21 up-regulation in hepatocytes Hepatology 49 2009 1176 1184
18. A.E. Feldstein, N.W. Werneburg, A. Canbay, M.E. Guicciardi, S.F. Bronk, and R. Rydzewski Free fatty acids promote hepatic lipotoxicity by stimulating TNF-alpha expression via a lysosomal pathway Hepatology 40 2004 185 194 (Pubitemid 38868937)
19. M.J. Gomez-Lechon, M.T. Donato, A. Martinez-Romero, N. Jimenez, J.V. Castell, and J.E. O'Connor A human hepatocellular in vitro model to investigate steatosis Chem Biol Interact 165 2007 106 116 (Pubitemid 46072796)
20. J. Araya, R. Rodrigo, L.A. Videla, L. Thielemann, M. Orellana, and P. Pettinelli Increase in long-chain polyunsaturated fatty acid n - 6/n - 3 ratio in relation to hepatic steatosis in patients with non-alcoholic fatty liver disease Clin Sci (Lond) 106 2004 635 643 (Pubitemid 38797577)
21. E. Fugassa, G. Gallo, A. Voci, and A. Cordone RNA synthesis in primary cultures of adult rat hepatocytes In Vitro 19 1983 299 306 (Pubitemid 15216022)
22. R. Koopman, G. Schaart, and M.K. Hesselink Optimisation of oil red O staining permits combination with immunofluorescence and automated quantification of lipids Histochem Cell Biol 116 2001 63 68 (Pubitemid 32695819)
23. K.J. Wiechelman, R.D. Braun, and J.D. Fitzpatrick Investigation of the bicinchoninic acid protein assay: identification of the groups responsible for color formation Anal Biochem 175 1988 231 237
24. S.M. Hsu, L. Raine, and H. Fanger Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures J Histochem Cytochem 29 1981 577 580 (Pubitemid 11147380)
25. K.M. Mak, C. Ren, A. Ponomarenko, Q. Cao, and C.S. Lieber Adipose differentiation-related protein is a reliable lipid droplet marker in alcoholic fatty liver of rats Alcohol Clin Exp Res 32 2008 683 689 (Pubitemid 351430433)
26. P. Chomczynski, and N. Sacchi Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction Anal Biochem 162 1987 156 159 (Pubitemid 17064313)
27. L. Vergani, C. Lanza, C. Borghi, L. Scarabelli, I. Panfoli, and B. Burlando Effects of growth hormone and cadmium on the transcription regulation of two metallothionein isoforms Mol Cell Endocrinol 263 2007 29 37 (Pubitemid 44828228)
28. M.W. Pfaffl A new mathematical model for relative quantification in real-time RT-PCR Nucleic Acids Res 29 2001 e45
29. H. Aebi Catalase in vitro Methods Enzymol 105 1984 121 126
30. J.M. McCord, and I. Fridovich Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein) J Biol Chem 244 1969 6049 6055
31. G.M. Small, K. Burdett, and M.J. Connock A sensitive spectrophotometric assay for peroxisomal acyl-CoA oxidase Biochem J 227 1985 205 210 (Pubitemid 15086962)
32. S.G. Ball, J. Sokolov, and W.W. Chin 3, 5-Diiodo-L-thyronine (T2) has selective thyromimetic effects in vivo and in vitro J Mol Endocrinol 19 1997 137 147 (Pubitemid 27431842)
33. E. Ferrannini, E.J. Barrett, S. Bevilacqua, and R.A. DeFronzo Effect of fatty acids on glucose production and utilization in man J Clin Invest 72 1983 1737 1747 (Pubitemid 14229053)
34. L. Everett, A. Galli, and D. Crabb The role of hepatic peroxisome proliferator-activated receptors (PPARs) in health and disease Liver 20 2000 191 199 (Pubitemid 30342716)
35. M. Yoon The role of PPARalpha in lipid metabolism and obesity: focusing on the effects of estrogen on PPARalpha actions Pharmacol Res 60 2009 151 159
36. S.E. Schadinger, N.L. Bucher, B.M. Schreiber, and S.R. Farmer PPARgamma2 regulates lipogenesis and lipid accumulation in steatotic hepatocytes Am J Physiol Endocrinol Metab 288 2005 E1195 205
37. O. Gavrilova, M. Haluzik, K. Matsusue, J.J. Cutson, L. Johnson, and K.R. Dietz Liver peroxisome proliferator-activated receptor gamma contributes to hepatic steatosis, triglyceride clearance, and regulation of body fat mass J Biol Chem 278 2003 34268 34276 (Pubitemid 37553270)
38. P. de Lange, A. Lombardi, E. Silvestri, F. Goglia, A. Lanni, and M. Moreno Peroxisome proliferator-activated receptor delta: a conserved director of lipid homeostasis through regulation of the oxidative capacity of muscle PPAR Res. 2008 2008 172676 172683
39. U. Edvardsson, M. Bergstrom, M. Alexandersson, K. Bamberg, B. Ljung, and B. Dahllof Rosiglitazone (BRL49653), a PPARgamma-selective agonist, causes peroxisome proliferator-like liver effects in obese mice J Lipid Res 40 1999 1177 1184 (Pubitemid 29328520)
40. M. Inoue, T. Ohtake, W. Motomura, N. Takahashi, Y. Hosoki, and S. Miyoshi Increased expression of PPARgamma in high fat diet-induced liver steatosis in mice Biochem Biophys Res Commun 336 2005 215 222 (Pubitemid 41253423)
41. C. Londos, C. Sztalryd, J.T. Tansey, and A.R. Kimmel Role of PAT proteins in lipid metabolism Biochimie 87 2005 45 49 (Pubitemid 40305232)
42. J.K. Reddy, and G.P. Mannaerts Peroxisomal lipid metabolism Annu Rev Nutr 14 1994 343 370 (Pubitemid 24261842)
43. H. Kasai, Y. Okada, S. Nishimura, M.S. Rao, and J.K. Reddy Formation of 8-hydroxydeoxyguanosine in liver DNA of rats following long-term exposure to a peroxisome proliferator Cancer Res 49 1989 2603 2605 (Pubitemid 19143385)
44. C.N. Mariash, and J.H. Oppenheimer Interrelationship of triiodothyronine concentration, metabolism, protein binding, and nuclear occupancy in the induction of malic enzyme by cultured adult rat hepatocytes Endocrinology 112 1983 80 85
45. E.J. Wilson, and W.C. McMurray Regulation of malic enzyme and mitochondrial alpha-glycerophosphate dehydrogenase by thyroid hormones, insulin, and glucocorticoids in cultured hepatocytes J Biol Chem 256 1981 11657 11662
46. S. Betley, M. Peak, and L. Agius Triiodo-L-thyronine stimulates glycogen synthesis in rat hepatocyte cultures Mol Cell Biochem 120 1993 151 158 (Pubitemid 23111174)