Use of capillary electrophoresis-sodium dodecyl sulfate to monitor disulfide scrambled forms of an Fc fusion protein during purification process

Analytical Biochemistry

Volumn 414, Issue 2, 2011, Pages 187-195

  Hapuarachchi, Suminda ^a   Fodor, Szilan ^a   Apostol, Izydor ^a   Huang, Gang ^a  

^a AMGEN INC   (United States)

Author keywords

Capillary electrophoresis; CE SDS; Disulfide scrambles; Fc fusion protein; Peptide map; Protein characterization; SDS PAGE

Indexed keywords

CAPILLARY ELECTROPHORESIS; ESCHERICHIA COLI; PURIFICATION; RECOMBINANT PROTEINS; REMOVAL; SULFUR COMPOUNDS;

DISULFIDE SCRAMBLES; FC FUSION PROTEINS; FUNCTIONAL PROTEINS; PROTEIN CHARACTERIZATION; PURIFICATION PROCESS; SDS PAGE; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; THERAPEUTIC PROTEIN;

SODIUM DODECYL SULFATE;

DISULFIDE; DODECYL SULFATE SODIUM; HYBRID PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; TRYPSIN;

ANION EXCHANGE; ARTICLE; CAPILLARY ELECTROPHORESIS; PEPTIDE MAPPING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION;

ESCHERICHIA COLI;

EID: 79956071108     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.03.017     Document Type: Article

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